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浙江大学学报(工学版)
J4  2012, Vol. 46 Issue (9): 1692-1696    DOI: 10.3785/j.issn.1008-973X.2012.09.022
化学工程     
大孔树脂对玉米蛋白粉中叶黄素的分离纯化
黄梅,周海胜,蒋凌志
浙江大学 化学工程与生物工程学系,浙江 杭州 310027
Separation and purification of xanthophylls from
corn gluten meal by macroporous adsorption resin
HUANG Mei, ZHOU Hai-sheng, JIANG Ling-zhi
Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China
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摘要：

通过比较H103、D4006、D4020、D14、S8、X5、AB8、HZ801、HZ816、DK110共10种树脂对玉米蛋白粉粗提液中叶黄素的静态吸附和解吸特征,发现 HZ816树脂对叶黄素组分不仅具有463.5 μg/g的高吸附量,而且解吸率达50%.采用Freundlich和Langmuir方程拟合20～40 ℃玉米蛋白粉中叶黄素的吸附等温线,结果表明:HZ816树脂对叶黄素的静态吸附符合Freundlich等温式.选择HZ816树脂用于玉米蛋白粉中叶黄素的固定床分离过程,在上样质量浓度79 μg/mL、上样速度1.1 BV/h、柱床层高度48 cm、乙醇洗脱的工艺条件下,得到叶黄素纯度为5.4%、收率达92.9%的产品.
Abstract:

To purify xanthophylls from corn gluten meal by resins. The static adsorption and desorption performances were investigated among H103, D4006, D4020, D14, S8, X5, AB8, HZ801, HZ816 and DK110 resins. The experimental results indicate that HZ816 resin is the desired adsorbent resin with the highest adsorption rate (4635 μg/g) and desorption capacity(50%).Freundlich and Langmuir equations were used to model the adsorption isotherms at equilibrium in the range of  20-40 ℃.The result shows that the Freundlich model fits the experimental data very adequately.The optimum conditions for the dynamic adsorption and desorption are as follows: concentration of sample solution 79 μg/mL, flow rate 1.1 BV/h, column height 48 cm, ethanol as the eluent. With these parameters, the purity of xanthophylls is increased to 5.4% with a recovery of 92.9%.
出版日期: 2012-09-01
:  TS 202.3  
作者简介: 黄梅(1969－), 女,副教授, 从事化工分离的教学科研工作. E-mail: huangm@zju.edu.cn
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引用本文:

黄梅,周海胜,蒋凌志. 大孔树脂对玉米蛋白粉中叶黄素的分离纯化[J]. J4, 2012, 46(9): 1692-1696.

HUANG Mei, ZHOU Hai-sheng, JIANG Ling-zhi. Separation and purification of xanthophylls from
corn gluten meal by macroporous adsorption resin. J4, 2012, 46(9): 1692-1696.

链接本文:

http://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2012.09.022    或    http://www.zjujournals.com/eng/CN/Y2012/V46/I9/1692

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SONG Yan, LI Ning. Role of xanthophylls and zeaxanthin in preventlng agerelated maeular degeneration［J］. Foreign Medical Sciences：Section of Hygiene, 2008, 35(5): 292-295．
［2］ 裴凌鹏, 惠伯棣. 玉米黄质改善D半乳糖致衰老大鼠抗氧化功能的研究［J］.老年医学与保健, 2008, 14(4): 217-219．
PEI Lingpeng, HUI Bodi. Zeaxanthin improves antioxidantive action in Dgalactose induced aging rats［J］. Geriatr Health Care,2008, 14(4): 217-219．
［3］ MARESPERLMAN J A, MILLEN A E, FICEK T L, et al. The body of evidence to support a protective role for lutein and zeaxanthin in delaying chronic disease. Overview ［J］. Journal of Nutrition, 2002, 132 (3): 518-52．

［4］ 许秀兰, 赵国华, 阚建全, 等.叶黄素研究进展［J］.粮食与油脂, 2004, 10: 3-7．
XU Xiulan,ZHAO Gnohua,KAN Jianquan, et al. Research advances of Lutein［J］. Cereals & Oils, 2004, 10: 3-7．
［5］ MOROS E E, DARAOKO D, CHERYAN M, et al. Analysis of xanthophylls in corn by HPLC［J］. Agricultural and Food Chemistry, 2002, 50: 5787-5790．
［6］ 张志华, 谌伟, 罗倩.玉米黄色素的提取工艺探讨［J］.中国食品添加剂, 2003(1): 27-30.
ZHANG Zhihua, CHEN Wei,LUO Qian. Study on extraction technology of yellow pigment from powder of zein［J］. China Food Additives, 2003(1): 27-30.
［7］ 王金恒, 展现明, 闫锁.玉米黄粉中色素及醇溶蛋白的提取工艺研究［J］. 安徽农业科学, 2009, 37(34):17080-17081,1708．
WANG Jinheng, ZHAN Xianming, YAN Suo. Research on the extraction of pigment and gliadin in corn［J］. Journal of Anhui Agricultural Sciences, 2009, 37(34):17080-17081,1708．
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LI Daqian, LIU Rong, WANG Ping. Extraction of yellow pigment from maize by supercritical CO2［J］. Journal of the Chinese Cereals and Oils Association, 2005(5): 35-39．
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LI Zisheng, CAI Muyi, YI Weixue. Study on enzymemodified proteins from Com Gluten Meal［J］. Food and Fermentation Industries, 2006, 32(4): 67-69.
［10］ LU Yanjie, YAO Huiyuan, GONG Yuansheng, et al. Effects of enzymatic treatment of Corn Gluten Meal on lutein and zeaxanthin extraction［J］. International Journal of Food Properties, 2005, 08: 161-169.
［11］ 周海胜, 蒋凌志, 黄梅. 蛋白酶预处理对玉米蛋白粉中叶黄素提取的影响［J］. 食品科学, 2010, 31(20):52-57.
ZHOU Haisheng, JIANG Lingzhi, HUANG Mei. Proteolytic pretreatment effects on the extraction of xanthophylls from Corn Gluten Meal［J］. Food Science, 2010, 31(20):52-57.
［12］ 刘品华, 李照刚, 魏柱平. 万寿菊花中提取胡萝卜素和叶黄素的研究［J］. 曲靖师范学院学报, 2003, 22(6): 40-42．
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［13］ 陈志强, 王洪亮. 大孔树脂分离纯化万寿菊中玉米黄质的研究［J］. 安徽农业科学, 2008, 36(2): 396-398．
LIU Zhiqiang, WANG Hongliang. Study on Macroporous Resin for Separation and Purification of Zeaxanthin in Marigold［J］. Journal of Anhui Agricultural Sciences, 2008, 36(2): 396-398．　　　　           
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