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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2023, Vol. 49 Issue (1): 105-116    DOI: 10.3785/j.issn.1008-9209.2022.02.142
Animal sciences & veterinary medicines     
Effects of the combination of sea cucumber peptide and Cistanche deserticola on hormone regulation and testicular anti-oxidative damage in acute-exercising mice
Xianliang LUO1(),Wangxin LIU1,Jing WANG1,Juan DU2,3,Fengqin FENG1()
1.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China
2.Hangzhou Kangyuan Food Technology Co. , Ltd. , Hangzhou 310003, Zhejiang, China
3.Zhejiang Nuoyan Biotechnology Co. , Ltd. , Huzhou 313000, Zhejiang, China
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Abstract  

The effects of the combination of sea cucumber peptide and Cistanche deserticola (SCPCD) on the hormone regulation and testicular anti-oxidative damage of acute-exercising mice were preliminarily explored in this study. The mice were randomly divided into five groups, including the blank group, the control group, SCPCD-low dose group (SCPCD-L) (0.3 mg/g), SCPCD-medium dose group (SCPCD-M) (0.6 mg/g), and SCPCD-high dose group (SCPCD-H) (2.0 mg/g), and all the mice received continuously intragastric administration by different doses of SCPCD for 42 d. An acute exhaustive swimming (AES) model was conducted, and a mating test was carried out on the 45th day. The sexual behavior ability parameters, hormone levels, and anti-oxidative related indexes were finally measured. The results showed that the optimal mass ratio of sea cucumber peptide and Cistanche deserticola in combination of them was 2∶1. AES led to the energy expenditure and accumulation of metabolites in mice (P＜0.05), but the acute exhaustive swimming time was prolonged and the contents of lactic acid and blood urea nitrogen were reduced by SCPCD treatments of mice. Besides, AES caused decreases in testosterone and estrogen contents, but they were significantly increased in the SCPCD-M and SCPCD-H (P＜0.05). The levels of follicle-stimulating hormone and irisin were significantly reduced (P＜0.01) in SCPCD-M, while the adrenocorticotropic hormone content was increased (P＜0.05). In addition, the sperm malformation rate decreased in SCPCD treatment, while the sperm motility and total antioxidant capacity of testis were significantly improved (P＜0.05). In summary, the SCPCD presented the physiological activities on delaying fatigue, improving testosterone level, and reducing the oxidative damage of sperm and testicular tissue in AES model. These results provide a scientific basis for the development and application of functional foods related to hormone regulation.

Key words： sea cucumber peptide      Cistanche deserticola      acute exhaustive swimming      hormone      oxidative damage     
Received: 14 February 2022      Published: 25 February 2023
CLC:  TS201.2  
Corresponding Authors: Fengqin FENG     E-mail: lxlsky008@163.com;fengfq@zju.edu.cn
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Xianliang LUO
Wangxin LIU
Jing WANG
Juan DU
Fengqin FENG
Cite this article:

Xianliang LUO,Wangxin LIU,Jing WANG,Juan DU,Fengqin FENG. Effects of the combination of sea cucumber peptide and Cistanche deserticola on hormone regulation and testicular anti-oxidative damage in acute-exercising mice. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(1): 105-116.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2022.02.142     OR     https://www.zjujournals.com/agr/Y2023/V49/I1/105


海参肽与肉苁蓉组合物对急性运动小鼠激素水平及睾丸抗氧化损伤的影响

为初步探究海参肽与肉苁蓉组合物对急性运动小鼠激素水平及睾丸抗氧化损伤的影响，将小鼠随机分为空白组、对照组、低剂量组合物组（0.3 mg/g）、中剂量组合物组（0.6 mg/g）和高剂量组合物组（2.0 mg/g），用不同剂量的海参肽与肉苁蓉组合物连续给小鼠灌胃42 d，采用急性力竭游泳造模，并于第45天进行交配实验，记录小鼠性行为学参数，并测定其激素水平及抗氧化相关指标。结果表明：组合物中海参肽与肉苁蓉的最佳质量比为2∶1。急性力竭游泳导致小鼠体内出现能量消耗和代谢产物堆积（P＜0.05），而海参肽与肉苁蓉组合物可不同程度地延长小鼠的急性力竭游泳时间，降低其体内乳酸和血尿素氮的含量。急性力竭游泳会引起小鼠睾酮和雌二醇含量下降，但中、高剂量组合物使两者含量显著提升（P＜0.05）；同时，中剂量组合物显著降低了小鼠促卵泡激素和鸢尾素水平（P＜0.01），并显著提高了其促肾上腺皮质激素含量（P＜0.05）；此外，各剂量组合物明显提高了小鼠精子活力，并且显著降低了小鼠精子畸形率，增强了睾丸组织的总抗氧化能力（P＜0.05）。综上所述，在小鼠急性力竭游泳模型中，海参肽与肉苁蓉组合物具有延缓疲劳症状产生、提高睾酮水平、减少精子和睾丸组织氧化损伤的生理活性。本研究为激素水平调节相关功能食品的开发与应用提供了科学依据。


关键词： 海参肽,  肉苁蓉,  急性力竭游泳,  激素,  氧化损伤 
Fig. 1 Molecular weight and amino acid composition of SCP and anti-oxidative capacity of SCPCD
Fig. 2 Experiment flowchart and the body mass and acute exhaustive swimming time of miceBlank group did not do acute exhaustive swimming training but participated in the mating test in figure A. CK: Control group; SCPCD-L: SCPCD-low dose group; SCPCD-M: SCPCD-medium dose group; SCPCD-H: SCPCD-high dose group (the same as below). Single asterisk (*) indicates significant differences between the experiment group and the control group at the 0.05 probability level.

组别

Group

脏器系数 Organ coefficient

肝脏

Liver

肾脏

Kidney

脾脏

Spleen

睾丸

Testis

精囊腺

Seminal vesicle

附睾

Epididymis

阴茎

Penis

空白组 Blank group4.48±0.291.54±0.140.26±0.070.77±0.070.64±0.150.34±0.050.10±0.02
对照组 CK4.60±0.491.53±0.070.28±0.050.81±0.080.64±0.160.35±0.050.09±0.03
低剂量组合物组 SCPCD-L4.84±0.261.58±0.180.30±0.080.78±0.130.76±0.170.36±0.080.10±0.02
中剂量组合物组 SCPCD-M4.80±0.281.61±0.130.30±0.070.81±0.100.73±0.130.36±0.070.11±0.02
高剂量组合物组 SCPCD-H4.51±0.441.55±0.130.24±0.050.83±0.120.68±0.050.35±0.060.11±0.02
Table 1 Organ coefficients of mice

组别

Group

肝糖原

Hepatic glycogen/(mg/g)

肌糖原

Muscle glycogen/(mg/g)

血尿素氮

BUN/(mmol/L)

乳酸

LA/(mmol/L)

一氧化氮

NO/(μmol/L)

空白组 Blank group0.41±0.060.61±0.093.62±0.615.09±0.4658.12±11.78
对照组 CK0.29±0.11#0.32±0.06##4.39±0.70#5.82±0.38#47.38±5.65
低剂量组合物组 SCPCD-L0.29±0.07#0.54±0.23*3.29±0.36**4.47±0.72**53.09±7.39
中剂量组合物组 SCPCD-M0.37±0.090.32±0.08##3.73±0.455.01±0.55*54.18±13.25
高剂量组合物组 SCPCD-H0.27±0.09#0.75±0.17**3.41±0.54**4.86±0.43**61.14±18.53
Table 2 Anti-fatigue related indexes of mice

组别

Group

骑跨潜伏期

Mounting latency/s

骑跨次数

Mounting frequency

射精次数

Ejaculation frequency

插入次数

Intromission frequency

空白组 Blank group568.20±335.304.86±3.843.60±1.8285.00±57.33
对照组 CK532.20±126.405.00±1.382.50±0.9653.71±20.63
低剂量组合物组 SCPCD-L494.00±118.709.62±2.745.17±1.42104.50±19.29
中剂量组合物组 SCPCD-M398.70±96.408.14±2.584.75±1.49128.20±71.03
高剂量组合物组 SCPCD-H564.80±132.607.00±1.513.14±0.63118.00±35.48
Table 3 Sexual behavior ability parameters of mice
Fig. 3 Serum hormone levels of mice
Fig. 4 Epididymis histopathology (A), sperm morphology (B), and sperm malformation rate (C)
Fig. 5 Histopathological analysis of testis
Fig. 6 Anti-oxidative indexes in testis of mice
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