Analysis of germplasm resources by simple sequence repeat markers in <i>Cymbidium hybridum</i>

doi:10.3785/j.issn.1008-9209.2021.03.082

Journal of Zhejiang University (Agriculture and Life Sciences)

2022, Vol. 48

Issue (1): 21-28 DOI: 10.3785/j.issn.1008-9209.2021.03.082

Horticultural sciences

Analysis of germplasm resources by simple sequence repeat markers in Cymbidium hybridum

Hongzhe SHEN¹(

),Chunnan LI²,Qiaojuan FU²,Hairui CUI¹(

)

^1.Institute of Nuclear-Agricultural Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
^2.Institute of Horticulture, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

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Abstract

Sixty-eight simple sequence repeat (SSR) primer pairs were designed based on the transcriptome data of Cymbidium faberi and 25 primer pairs with stable amplification and better polymorphism were selected for genetic diversity analysis of 41 C. hybridum cultivars. The results showed that a total of 187 SSR bands were amplified, and 171 (91.44%) of them were polymorphic with the average of 6.84 bands per primer pair. The mean value of polymorphism information content (PIC) and the number of genotypes per SSR marker were 0.770 3 and 12.88, respectively. Genetic similarity coefficient among 41 cultivars ranged from 0.57 to 0.99 with an average of 0.72. A dendrogram of genetic relationship constructed using unweighted pair-group method with arithmetic mean (UPGMA) showed that the tested accessions could be classified into five clustering groups with the similarity coefficient of 0.71, which were consistent to some extent with the plant size, branch type and flower color. The above results reveal the genetic diversity and relationships among the tested accessions. The newly developed SSR markers will be useful tools for molecular breeding of C.hybridum.

Key words： Cymbidium hybridum simple sequence repeat genetic diversity cluster analysis

Received: 08 March 2021 Published: 04 March 2022

CLC:

Q 75

Corresponding Authors: Hairui CUI E-mail: 21816004@zju.edu.cn;hrcui@zju.edu.cn

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Cite this article:

Hongzhe SHEN,Chunnan LI,Qiaojuan FU,Hairui CUI. Analysis of germplasm resources by simple sequence repeat markers in Cymbidium hybridum. Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(1): 21-28.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2021.03.082 OR https://www.zjujournals.com/agr/Y2022/V48/I1/21

大花蕙兰种质资源的简单重复序列标记分析

利用蕙兰（Cymbidium faberi）转录组数据设计了68对微卫星即简单重复序列（simple sequence repeat, SSR）标记引物，从中筛选25对扩增稳定、多态性好的引物，并对41个大花蕙兰（C. hybridum）品种的遗传多样性进行了分析。结果显示：试验共扩增出187个条带，其中171条（占91.44%）为多态性条带，平均每对引物扩增多态性条带6.84条；平均每对SSR标记显示的多态性信息含量值和基因型数分别为0.770 3和12.88。41份大花蕙兰种质资源之间的遗传相似系数为0.57～0.99，平均值为0.72。利用非加权成对算术平均法建立了聚类图，在遗传相似系数0.71处可将供试材料分为5类，分类结果与植株大小、花枝类型及花色等性状相关。上述分析结果较好地揭示了大花蕙兰品种间的遗传多样性与亲缘关系，这些新开发的SSR标记可为大花蕙兰分子育种提供有用的工具。

关键词： 大花蕙兰, 简单重复序列, 遗传多样性, 聚类分析

Table 1 Cultivars and characteristics of C. hybridum used in this study

Table 2 Frequency of SSR in C. faberi unigene sequences

Fig. 1 Agarose gel electrophoresis of PCR products from some SSR primer pairsM: 100-600 bp DNA marker; 1-12: PCR products of primers from SSR10 to SSR21.

Table 3 Amplification of 25 polymorphic primer pairs

Table 4 Specific SSR bands showed in some cultivars

Fig. 2 Dendrogram constructed for 41 germplasms based on UPGMAPlease see the Table 1 for the cultivar name represented by the number in this figure.


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