NLRC5 sequence characteristics and analysis of tissue expression profile in goose (<i>Anser cygnoides</i>)

doi:10.3785/j.issn.1008-9209.2021.03.012

Journal of Zhejiang University (Agriculture and Life Sciences)

2022, Vol. 48

Issue (2): 254-260 DOI: 10.3785/j.issn.1008-9209.2021.03.012

Animal sciences & veterinary medicines

NLRC5 sequence characteristics and analysis of tissue expression profile in goose (Anser cygnoides)

Shuai ZHAO¹(

),Tiantian GU¹,Li’e HOU¹,Yang ZHANG¹,Zhengfeng CAO¹,Guohong CHEN^1,²,Qi XU¹,Yu ZHANG¹(

)

^1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
^2.Joint International Research Laboratory of Agriculture & Agri-Product Safety, Ministry of Education, Yangzhou University, Yangzhou 225009, Jiangsu, China

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Abstract

As one of the important members of the NOD-like receptor (NLR) family, NLRC5 plays an important role in the recognition of invading microorganisms, immune signal transmission and innate immune response regulation in vivo, but there are relatively few researches on NLRC5 in poultry. In this study, the Yangzhou goose was selected, and the sequence characteristics of NLRC5 was analyzed, and the expression profile of NLRC5 gene was detected by real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). The expression levels of NLRC5 in the liver, spleen and kidney of goslings infected with Salmonella enteritidis at 0, 1, 2 and 4 d post-infection were examined. The results showed that the amino acid sequence of goose NLRC5 was highly conserved in vertebrates, and shared high homology with duck (89.12%). The NLRC5 mRNA was widely expressed in the heart, liver, spleen, lung, kidney, duodenum, gizzard, brain and muscle, and its expression level was the highest in the spleen. In addition, the expressions of NLRC5 in the liver, spleen and kidney of goslings infected with S.enteritidis were significantly upregulated during 1-2 d, and then returned to normal at 4 d post-infection. The above results are helpful to understand the role of NLRC5 in the immune response of goose infected with S. enteritidis.

Key words： NLRC5 goose innate immune response Salmonella enteritidis

Received: 01 March 2021 Published: 29 April 2022

CLC:

S 835

Corresponding Authors: Yu ZHANG E-mail: 626981977@qq.com;yuzhang@yzu.edu.cn

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	Shuai ZHAO
	Tiantian GU
	Li’e HOU
	Yang ZHANG
	Zhengfeng CAO
	Guohong CHEN
	Qi XU
	Yu ZHANG

Cite this article:

Shuai ZHAO,Tiantian GU,Li’e HOU,Yang ZHANG,Zhengfeng CAO,Guohong CHEN,Qi XU,Yu ZHANG. NLRC5 sequence characteristics and analysis of tissue expression profile in goose (Anser cygnoides). Journal of Zhejiang University (Agriculture and Life Sciences), 2022, 48(2): 254-260.

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https://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2021.03.012 OR https://www.zjujournals.com/agr/Y2022/V48/I2/254

鹅NLRC5序列特征及组织表达谱分析

作为NOD样受体（NOD-like receptor, NLR）家族重要成员之一的NLRC5，在机体识别入侵微生物、传递免疫信号及调节先天性免疫反应中具有重要作用。本研究以扬州鹅为研究对象，分析鹅NLRC5序列特征，并通过实时荧光定量聚合酶链反应（real-time fluorescent quantitative polymerase chain reaction, RT-qPCR）检测NLRC5基因的组织表达谱，同时检测雏鹅感染肠炎沙门菌0、1、2、4 d后肝脏、脾脏和肾脏中NLRC5的表达水平。结果显示：鹅NLRC5氨基酸序列在脊椎动物中高度保守，其中鹅与鸭NLRC5序列同源性为89.12%；NLRC5基因在心脏、肝脏、脾脏、肺、肾脏、十二指肠、肌胃、大脑和肌肉等组织中均有表达，其中在脾脏中的表达水平最高；此外，雏鹅感染肠炎沙门菌后其肝脏、脾脏和肾脏中NLRC5的表达量在1～2 d内显著上升（P＜0.05），然后在感染4 d内恢复至正常水平。上述研究结果有助于理解NLRC5在鹅感染肠炎沙门菌免疫应答中的作用。

关键词： NLRC5, 鹅, 先天性免疫反应, 肠炎沙门菌

Table 1 Primer information for RT-qPCR

Fig. 1 Analysis of NLRC5 amino acid sequence

Table 2 Homology alignment of NLRC5 amino acid sequences of various species

Fig. 2 Phylogenetic tree analysis of NLRC5 gene

Fig. 3 Expression profiles of NLRC5 mRNA in goose tissuesDifferent lowercase letters above bars indicate significant differences at the 0.05 probability level, and the same below.

Fig. 4 Changes of NLRC5 mRNA expression in different tissues of goslings after being infected with S.enteritidisA. Liver; B. Spleen; C. Kidney.


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