| Animal sciences & veterinary medicine |
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| Determination of the volatile fatty acid contents in rumen fluid of ruminants by headspace-gas chromatograph method |
Junhong WANG( ),Ge GAO,Yuanyuan ZHANG,Ying SHAN,Weiren DONG() |
| College of Animal Sciences, Zhejiang University, Hangzhou 310058, China |
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Abstract A headspace-gas chromatograph (HS-GC) (external standard method) was established for the determination of the volatile fatty acid (VFA) contents in rumen fluid of ruminants. Under the optimized headspace sampling conditions (balance temperature at 85 ℃ and equilibrium time of 30 min), the method was verified and used to detect the VFA. The coefficient of determination of regression equation for each compound was not less than 0.999 7. The limit of detection (LOD) was 0.036-0.329 mmol/L. The recovery rates of three different concentrations of standards were 93.540%-108.130%. The detection results showed that the relative standard deviations (RSDs) of VFA in all samples were less than 2.3%, and the method was stable within three days. The developed method is simple, which has accurate quantity and good repeatability, and suitable for the quantitative analysis of VFA in rumen fluid of ruminants.
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Received: 20 November 2020
Published: 27 October 2021
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Corresponding Authors:
Weiren DONG
E-mail: jhwang@zju.edu.cn;dongweiren@zju.edu.cn
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顶空-气相色谱法测定反刍动物瘤胃液中挥发性脂肪酸含量
利用顶空-气相色谱检测方法（外标法）测定反刍动物瘤胃液中挥发性脂肪酸含量，并对顶空进样条件进行优化。在最佳平衡温度（85 ℃）和平衡时间（30 min）的顶空进样条件下，各挥发性脂肪酸的线性关系良好，回归方程决定系数均不小于0.999 7，检出限为0.036～0.329 mmol/L，样品的加标回收率为93.540%～108.130%。检测结果表明，样品中挥发性脂肪酸浓度的相对标准偏差均小于2.3%，3 d内稳定性良好。该方法操作简单、定量准确、重复性好，适用于反刍动物瘤胃液中挥发性脂肪酸的快速检测。
关键词：
顶空-气相色谱,
反刍动物瘤胃液,
挥发性脂肪酸,
外标法
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