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浙江大学学报（农业与生命科学版）
Journal of Zhejiang University (Agriculture and Life Sciences)  2021, Vol. 47 Issue (1): 118-126    DOI: 10.3785/j.issn.1008-9209.2020.05.201
Animal sciences & veterinary medicine     
Effects of tea polyphenols on anti-inflammation and promotion of wound healing and its mechanisms
Hangye ZHAO1(),Chen XIA1,2,Puming HE1,Youying TU1()
1.Department of Tea Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
2.Hangzhou Easily Biotechnology Co. , Ltd. , Hangzhou 310015, China
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Abstract  

This study focuses on the effects of tea polyphenols (TPPs) on anti-inflammation and promoting wound healing in vivo and its mechanisms. ICR mice were selected as animal materials to make the wound. The wounded mice were treated with 0.3%, 0.5%, 0.8% and 1.0% TPPs, respectively. The treatment with artificial cell healing membrane was used as the positive control, and the blank control was set up. The effects of different concentrations of TPPs on the immune system and granulation tissue of wounded mice were measured after 9 d. The results showed that 0.5% TPPs could accelerate the wounded mice healing and the healing speed of this treatment was faster than the positive control. Meanwhile, 0.3% and 0.5% TPPs treatment groups could reduce the concentrations of tumor necrosis factor-α (TNF-α)and interleukin-2 (IL-2) in the late stage of wound healing to prevent excessive inflammatory reaction. Superoxide dismutase (SOD) activity in wound tissues was decreased significantly by the TPPs treatment as compared with the blank control group, which showed that TPPs may help to eliminate excessive free radicals in wound, and then to prevent its damage to the body. Hematoxylin-eosin (HE) staining sections of granulation tissue at the wound showed that TPPs treatment could promote the formation of capillaries in the wound. The function of TPPs in promoting wound healing was related to its concentration. At lower concentrations (below 0.5%), with the increase of concentration of TPPs, the ability of promoting wound healing increased; at higher concentrations (above 0.8%), TPPs showed the physiological characteristics of inhibiting wound healing. To some extent, the experimental results elucidate the partial mechanisms of promoting wound healing and anti-inflammation by TPPs and find that the suitable concentration range of TPPs for promoting wound healing is 0.3%-0.8%.

Key words： tea polyphenols      ICR mice      immune system      granulation tissue      wound healing     
Received: 20 May 2020      Published: 09 March 2021
CLC:  S  
Corresponding Authors: Youying TU     E-mail: hangyzhao@zju.edu.cn;youytu@zju.edu.cn
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Hangye ZHAO
Chen XIA
Puming HE
Youying TU
Cite this article:

Hangye ZHAO,Chen XIA,Puming HE,Youying TU. Effects of tea polyphenols on anti-inflammation and promotion of wound healing and its mechanisms. Journal of Zhejiang University (Agriculture and Life Sciences), 2021, 47(1): 118-126.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2020.05.201     OR     http://www.zjujournals.com/agr/Y2021/V47/I1/118


茶多酚抗炎和促外伤愈合作用及其机制

为研究茶多酚（复合物）在活体动物伤口愈合过程中的作用及其机制，试验选取ICR小鼠作为试验材料，进行伤口造模，并使用质量分数分别为0.3%、0.5%、0.8%、1.0%的茶多酚溶液对伤口进行处理，以人工细胞愈合膜处理作为阳性对照，并设置空白对照组，于9 d后测定不同含量茶多酚对受伤小鼠免疫系统以及伤口处肉芽组织的影响。结果表明：0.5%茶多酚处理能加速小鼠伤口愈合，其愈合情况优于阳性对照组；同时，0.3%、0.5%茶多酚处理在伤口愈合后期可降低肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）、白细胞介素-2（interleukin-2, IL-2）等细胞因子水平，防止过度的炎症反应。通过对伤口处超氧化物歧化酶（superoxide dismutase, SOD）水平的测定发现，茶多酚处理组的SOD活性较空白对照组显著降低，表明其可能对伤口处因炎症反应而产生的自由基的清除有一定作用，从而阻止其对机体的损害；对伤口处肉芽组织进行苏木精-伊红染色、切片观察发现，茶多酚处理可促进伤口处毛细血管的形成。同时，茶多酚（复合物）表现出的促外伤愈合功能与其含量有关。在较低质量分数（0.5%以下）时，随着茶多酚含量的升高，其促外伤愈合能力增强；而在较高质量分数（0.8%以上）时，茶多酚表现出抑制伤口愈合的生理特性。该试验结果在一定程度上阐明了茶多酚的部分抗炎和促外伤愈合机制，并发现促进创面愈合的茶多酚的适宜质量分数范围为0.3%～0.8%。


关键词： 茶多酚,  ICR小鼠,  免疫系统,  肉芽组织,  伤口愈合 
Fig. 1 Eye observation results of the back wound in mice

处理组

Treatment group

9 d后伤口面积平均缩小比例

Reduction rate of the wound

area after 9 d/%

阳性对照 Positive control75.30±3.24a
空白对照 Blank control64.40±4.73b
0.3%茶多酚 0.3% TPPs79.30±3.21a
0.5%茶多酚 0.5% TPPs77.07±6.67a
0.8%茶多酚 0.8% TPPs57.84±8.22bc
1.0%茶多酚 1.0% TPPs43.83±9.33c
Table 1 Reduction rates of the wound area in each treatment group

处理组

Treatment group

ρ（TNF-α）/

(pg/mL)

ρ（IL-2）/

(pg/mL)

阳性对照 Positive control110.20±1.10b25.43±1.75d
空白对照 Blank control129.68±3.46a33.36±2.32a
0.3%茶多酚 0.3% TPPs124.68±17.19ab29.66±1.76bc
0.5%茶多酚 0.5% TPPs111.30±16.97b27.87±0.74c
0.8%茶多酚 0.8% TPPs121.33±3.27ab30.80±1.44b
1.0%茶多酚 1.0% TPPs130.99±19.33a28.83±1.33bc
Table 2 Concentrations of TNF-α and IL-2 in mice serum of each treatment group

处理组

Treatment group

ρ（SOD）/(pg/mL)
阳性对照 Positive control434.72±10.23ab
空白对照 Blank control445.39±20.05a
0.3%茶多酚 0.3% TPPs429.16±4.33b
0.5%茶多酚 0.5% TPPs393.83±6.57c
0.8%茶多酚 0.8% TPPs400.00±9.96c
1.0%茶多酚 1.0% TPPs413.62±4.05bc
Table 3 Concentrations of SOD in mice serum of each treatment group
Fig. 2 HE staining sections of granulation tissueA. Positive control group; B. Blank control group; C. 0.3% tea polyphenols (TPPs) treatment group; D. 0.5% TPPs treatment group; E. 0.8% TPPs treatment group; F. 1.0% TPPs treatment group. BV: Blood vessel; Fib: Fibroblast; CF: Collagenous fiber bundle.
Fig. 3 Number of fibroblasts and blood vesselsA. Positive control group; B. Blank control group; C. 0.3% tea polyphenols (TPPs) treatment group; D. 0.5% TPPs treatment group; E. 0.8% TPPs treatment group; F. 1.0% TPPs treatment group. Different lowercase letters above bars represent significant differences at the 0.05 probability level; n=8.
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