| Animal sciences & veterinary medicine |
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| Residue depletion laws of florfenicol and its metabolite florfenicol amine in eggs |
Jing ZHANG( ),Qian ZHOU,Yushi GAO(),Xiujun TANG,Junxian LU,Lina MA,Dawei CHEN,Mengjun TANG |
| Jiangsu Institute of Poultry Sciences, Yangzhou 225125, Jiangsu, China |
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Abstract A study on florfenicol (FF) and florfenicol amine (FFA) residue depletion raws was conducted in eggs. After the chickens were administered successively florfenicol (FF) powder of 15 mg/kg (prevention group) and 30 mg/kg (treatment group) of body mass in the form of premix for 5 d, the eggs of each group were collected at administration period and 1, 2, 3, 5, 7, 10, 12, 15, 18 d at withdrawal period. Then FF and FFA residues of egg, egg yolk and egg white were detected using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results showed that the limits of detection (LOD) of FF and FFA in egg, egg yolk and egg white were 2 μg/kg, and the limits of quantitation (LOQ) were 5 μg/kg. The average recoveries were in the range of 78.9%-105.0% when the egg samples were added with FF in the range of 1.0-5.0 μg/kg, and the intraday precision and the interday precision was 2.22%-4.22% and 4.22%-6.54%, respectively. The peak values of FF residues (520.70, 797.12 μg/kg) of egg white in prevention group and treatment group, respectively, were higher than those of egg yolk (285.96, 442.40 μg/kg), while the peak values of FFA residue (218.40, 646.14 μg/kg) of egg yolk in prevention group and treatment group, respectively, were higher than those of egg white (87.36, 36.89 μg/kg). The elimination rates of FF and FFA in egg white were faster than those in egg yolk. FF and FFA residues and their total residues were positively correlated with the dosage. FF and FFA mainly existed in the egg yolk, and FF mainly existed in the egg white. The FF residual time of egg yolk in different dosage groups was 18 d.
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Received: 24 March 2020
Published: 09 March 2021
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Corresponding Authors:
Yushi GAO
E-mail: zhangjing9143@126.com;gaoys100@sina.com
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氟苯尼考及其代谢物氟苯尼考胺在鸡蛋中的残留消除规律
本试验旨在研究氟苯尼考及其代谢物氟苯尼考胺在鸡蛋中的残留消除规律。预防组和治疗组蛋鸡分别按体质量以15和30 mg/kg剂量预拌料方式给予氟苯尼考粉药物，连续5 d，分别收集给药期和停药后第1、2、3、5、7、10、12、15、18天的各组鸡蛋，测定全蛋、蛋黄和蛋清中氟苯尼考和氟苯尼考胺的残留量。结果表明：全蛋、蛋清和蛋黄中氟苯尼考和氟苯尼考胺的检出限均为2 μg/kg，定量限均为5 μg/kg。鸡蛋样品在氟苯尼考添加水平为1.0～5.0 μg/kg时平均回收率为78.9%～105.0%，日内精密度为2.22%～4.22%，日间精密度为4.22%～6.54%。停药后，氟苯尼考在预防组和治疗组蛋清中的残留量峰值（520.70、797.12 μg/kg）高于蛋黄（285.96、442.40 μg/kg），氟苯尼考胺在蛋黄中的残留量峰值（218.40、646.14 μg/kg）高于蛋清（87.36、36.89 μg/kg），氟苯尼考和氟苯尼考胺在蛋清中的消除速率均快于蛋黄。氟苯尼考和氟苯尼考胺残留量及其总残留量与给药剂量呈正相关，蛋黄中以氟苯尼考和氟苯尼考胺残留为主，蛋清中主要是氟苯尼考残留。不同剂量组蛋黄中氟苯尼考残留时间为18 d。
关键词：
氟苯尼考,
氟苯尼考胺,
鸡蛋,
残留消除,
高效液相色谱-串联质谱法
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