Research advances in polysaccharide utilization loci of rumen microorganism

doi:10.3785/j.issn.1008-9209.2019.07.091

Journal of Zhejiang University (Agriculture and Life Sciences)

2020, Vol. 46

Issue (3): 263-270 DOI: 10.3785/j.issn.1008-9209.2019.07.091

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Research advances in polysaccharide utilization loci of rumen microorganism

Ge GAO(

),Jiakun WANG(

)

Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

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Abstract

Polysaccharide utilization loci (PULs) are gene clusters that orchestrate the breakdown of a specific glycan, encode cell surface polysaccharide binding proteins, outer membrane transport proteins, carbohydrate-active enzymes (CAZymes) and transcription factors. Bacteroidetes are highly abundant in rumen and are considered as efficient degraders of polysaccharides, which can use PULs to arrange the detection, sequestration, digestion of complex carbohydrates. Effective improvement of the rumen function and excavation of high-performance enzymes by Bacteroidetes will be significantly informed by a holistic understanding of the mechanisms of PULs. This paper introduces the mode of action and regulatory mechanism of PULs, reviews the latest developments in rumen PULs research, and is aimed at providing the theoretical basis for the strengthening of PULs study and the application of PULs in microorganism modification and bioenergy development.

Key words： rumen microorganism Bacteroidetes polysaccharide polysaccharide utilization loci

Received: 09 July 2019 Published: 17 July 2020

CLC:

S 811.6

Corresponding Authors: Jiakun WANG E-mail: gloria1942@163.com;jiakunwang@zju.edu.cn

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Cite this article:

Ge GAO,Jiakun WANG. Research advances in polysaccharide utilization loci of rumen microorganism. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(3): 263-270.

URL:

http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2019.07.091 OR http://www.zjujournals.com/agr/Y2020/V46/I3/263

瘤胃微生物多糖利用位点研究进展

多糖利用位点（polysaccharide utilization loci, PULs）是一组编排特定多糖降解的基因簇，编码细胞表面多糖结合蛋白、外膜转运蛋白、碳水化合物活性酶和转录因子。通过多糖利用位点，拟杆菌可更好地协同多个蛋白的合作，实现对植物多糖识别、捕获和降解的一体化，具备高效利用多糖的能力。拟杆菌在瘤胃微生物中占比丰富，揭示瘤胃拟杆菌通过多糖利用位点降解纤维类物质的作用机制是改善瘤胃功能、挖掘高效酶的基础。本文主要对多糖利用位点的作用模式、调控机制及瘤胃微生物多糖利用位点的研究进展进行了综述，旨在为加强多糖利用位点的研究，并将其应用于微生物调控和生物能源开发提供理论依据。

关键词： 瘤胃, 微生物, 拟杆菌, 多糖, 多糖利用位点

Fig. 1 Function diagram of a PUL^[12]Sus: Starch utilization system; GH: Glycoside hydrolase; PUL: Polysaccharide utilization locus; Reg.: Regulator.

Fig. 2 Substrate selection diagram of PULs in rumenPUL: Polysaccharide utilization locus; Reg.: Regulator; GH: Glycoside hydrolase; Sus: Starch utilization system; Trans.: Transporter; Epim.: Mannobiose-2-epimerase; CE: Carbohydrate esterase; Hypo.: Hypothetical protein; Est.: Esterase.

Table 1 PULs detected in rumen Prevotella ruminicola and Prevotella bryantii strains^[40]


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