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浙江大学学报（农业与生命科学版）
  2012, Vol. 38 Issue (4): 519-523    DOI: 10.3785/j.issn.1008-9209.2012.04.022
Food sciences     
Deproteinization method and optimal condition of crude polysaccharide from Lachnum sp
SUN Zhe1, QIU Tao1, YUAN Ruyue1, QIAN Meishuang1, XIAO Hourong2, YE Ming1
1.College of Biotechnology and Food Engineering, HeFei University of Technology, Hefei 230009, China；2. Department of Biological and Environmental Engineering, Hefei University, Hefei 230022, China
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Abstract  Sevage method, TCA-NBA (trichloroacetic acidNbutyl alcohol) method, enzymeSevage method and enzymeTCA-NBA method were used to remove protein from extracellular polysaccharide of Lachnum  YM281, respectively. The results showed that the method of enzymeTCA-NBA was the most effective, by which the proteinremoval rate and the polysaccharideloss rate were 51.57% and 3.46%, respectively. Then the uniform design was employed to optimize the conditions of this method. As a result, the optimal conditions of the  enzymeTCA-NBA method were as follows: polysaccharide solution of Lachnum YM281 of 20 mL, papain solution of 11 mL, pH 4, zymolysis time of 3.5 h at 42.2 ℃; after removing the denatured protein, TCA-NBA method was used remove protein twice. Under this optimal method and conditions, the proteinremoval rate reached up to 72.3% and the polysaccharideloss rate was only 2.93%．

Published: 25 July 2012
CLC:  TQ929.2  
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SUN Zhe1
QIU Tao1
YUAN Ruyue1
QIAN Meishuang1
XIAO Hourong2
YE Ming1
Cite this article:

SUN Zhe1, QIU Tao1, YUAN Ruyue1, QIAN Meishuang1, XIAO Hourong2, YE Ming1. Deproteinization method and optimal condition of crude polysaccharide from Lachnum sp. , 2012, 38(4): 519-523.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.04.022     OR     http://www.zjujournals.com/agr/Y2012/V38/I4/519


粒毛盘菌多糖脱蛋白方法及其条件优化

分别采用Sevage法、三氯乙酸正丁醇（TCA-NBA）法、酶Sevage法以及酶TCA-NBA法对粒毛盘菌YM281胞外多糖进行脱蛋白。结果表明：用酶TCA-NBA法对粒毛盘菌YM281胞外多糖脱蛋白的效果最佳，蛋白脱除率为51.57%，且此时多糖损失率（3.46%）为最低；进一步采用均匀设计优化该方法的脱蛋白条件，所得最优脱蛋白条件为：粒毛盘菌YM281多糖溶液20 mL、木瓜蛋白酶溶液11 mL、温度42.2 ℃、pH 4、酶解时间3.5 h；除去变性蛋白后，再利用TCA-NBA法脱蛋白2次即可；采用所得的最优方法和条件脱蛋白，其蛋白脱除率高达72.3%，多糖损失率仅为2.93%。
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