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浙江大学学报（农业与生命科学版）
  2012, Vol. 38 Issue (1): 28-34    DOI: 10.3785/j.issn.1008-9209.2012.01.004
Biological sciences & biotechnology     
Analysis of differentially expressed genes of Rhizoctonia solani during the sclertium formation
CHEN Xiao‐yao , WANG Wei , WANG Zheng‐yi , CHEN Wei‐liang
Institute o f Biotechnology ,Zhe jiang University , Hang zhou 310058 , China
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Abstract  　Subtractive cDNA library of differentially expressed genes during the sclertium formation of
Rhizoctonia solani , GD‐１１８ strain , was constructed by using suppression subtractive hybridization
(SSH) technique combined with virtual Northern blot . The hypha mRNA was used as the driver and the
sclertium mRNA was applied as the tester . Further PCR screening showed that the size of the inserts
was mainly between ２００ to ５００ bp . The effectiveness of the subtractive hybridization was verified by
virtual Northern blot of colonies randomly selected using hypha , sclertium cDNA sequence as probes .
Finally , ２８ specific sclertium formation‐related gene fragments were obtained after sequencing and
homology‐blasting , which were mainly related to stress response , transcription regulation , signal
transduction and metabolism in the pathogen . Results above provide scientific clues to understand the
mechanism of the sclertium formation of R . solani .

Published: 20 January 2012
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CHEN Xiao yao
WANG Wei
WANG Zheng yi
CHEN Wei liang
Cite this article:

CHEN Xiao yao,WANG Wei,WANG Zheng yi,CHEN Wei liang. Analysis of differentially expressed genes of Rhizoctonia solani during the sclertium formation. , 2012, 38(1): 28-34.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.01.004     OR     http://www.zjujournals.com/agr/Y2012/V38/I1/28


水稻纹枯病菌菌核形成过程中特异基因的表达与分析

　以水稻纹枯病GD‐１１８ 菌株菌丝形成期的mRNA 为驱赶子(driver) ,以菌核形成期的mRNA 为
检测子(tester) ,采用抑制消减杂交(SSH)结合虚拟Northern 杂交筛选,构建了水稻纹枯病菌菌核形成
过程中特异表达产生的差减cDNA 文库;进一步用PCR 筛选显示克隆中插入的片段主要分布在２００ ～
５００ bp 之间;随机挑取克隆应用虚拟Northern 技术以菌丝、菌核cDNA 为探针来验证消减有效性,经测
序和同源性检测剔除重复序列之后,获得了从菌丝到菌核形成相关的２８ 条特异表达的基因片段;所筛
选的特异表达序列主要涉及胁迫响应、转录调控、信号传导、合成代谢等.这为进一步分析水稻纹枯病菌
菌核形成过程的相关基因提供了科学线索.
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