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浙江大学学报（农业与生命科学版）
  2012, Vol. 38 Issue (1): 21-27    DOI: 10.3785/j.issn.1008-9209.2012.01.003
Biological sciences & biotechnology     
Functional analysis of an ubiquitin fusion degradation protein gene UFD1 in regulation of plant disease andstress resistance
LAI Yi‐yu１ , LI Fei１ , XU You‐ping２ , CAI Xin‐zhong１ ,３
1 . Institute o f Biotechnology , Zhej iang
University , H angz hou 310058 , China ; 2 . Centre o f A nalysis and Measurement , Zhej iang University ,
H angz hou 310058 , China ; 3 . K ey L aboratory o f Molecular Biology o f Crop Pathogens and Insects ,
M inistry o f A griculture , Zhej iang University , H angz hou 310058 , China
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Abstract  　A fragment of ubiquitin fusion degradation protein (UFD) gene UFD1 was cloned from tomato
by reverse transcription‐polymerase chain reaction (RT‐PCR) , and its role in regulation of plant disease
and stress resistance was analyzed employing virus‐induced gene silencing (VIGS) technique . The results
showed that the silencing of UFD1 in N icotiana benthamiana resulted in significantly weakening of
hypersensitive response ( HR) mediated by C f‐4/ A v r4‐ and C f‐9/A v r9 and induced by X anthomonas
oryz ae pv . oryz ae (Xoo) , alleviating of necrotic symptoms of tobacco wild fire disease , decreasing of
chlorophyll content in leaves under stress of high concentration of salt , but did not lead to change of
symptoms of white mould disease caused by Sclerotinia sclerotiorum and resistance to drought stress . It is indicated that UFD1 is a pivotal regulator of plant disease and stress resistance , playing roles in
positive regulation of C f‐4‐ and C f‐9‐dependent gene‐for‐gene resistance , nonhost resistance to Xoo and
salt tolerance , and in contrast , negative regulation of resistance to tobacco wild fire disease .

Published: 20 January 2012
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LAI Yi yu
LI Fei
XU You ping
CAI Xin zhong
Cite this article:

LAI Yi yu,LI Fei,XU You ping,CAI Xin zhong. Functional analysis of an ubiquitin fusion degradation protein gene UFD1 in regulation of plant disease andstress resistance. , 2012, 38(1): 21-27.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2012.01.003     OR     http://www.zjujournals.com/agr/Y2012/V38/I1/21


泛素融合降解蛋白基因UFD1对植物抗病和抗逆性的调控功能分析

采用反转录‐聚合酶链反应( reverse transcription‐polymerase chain reaction , RT‐PCR)克隆获得１
个番茄泛素融合降解蛋白(ubiquitin fusion degradation protein , UFD) 基因片段UFD1 ,并应用病毒诱导
的基因沉默(virus‐induced gene silencing , VIGS) 技术分析该基因对植物抗病性和抗逆性的调控功能.
结果表明:在本氏烟( N icotiana benthamiana)植株中UFD1 基因的沉默导致C f‐4/ A v r4 和C f‐9/ A v r9
介导的过敏性反应( hypersensitive response , HR) 以及水稻白叶枯病菌( X anthomonas oryz ae pv .
oryz ae , Xoo)诱导的HR 显著减弱,烟草野火病坏死症状明显减轻,在高盐胁迫下叶片叶绿素含量显著
降低,但对菌核病病害症状以及抗干旱能力的影响不明显.表明UFD1 基因是植物抗病和抗逆性的重
要调控因子,它可能对C f‐4/C f‐9 介导的基因对基因抗性、对Xoo 的非寄主抗性和耐盐性起正向调控
作用,而对烟草野火病抗性起负调控作用.
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