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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2023, Vol. 49 Issue (5): 633-643    DOI: 10.3785/j.issn.1008-9209.2023.06.171
作物重要细菌和病毒病害专题     
维管束木质化调控植物抗青枯病的研究进展
李陈莹(),王冉,梁岩()
浙江大学农业与生物技术学院生物技术研究所，浙江 杭州 310058
Research progress on the regulation of vascular lignification on defense against bacterial wilt of plants
Chenying LI(),Ran WANG,Yan LIANG()
Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China
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摘要：

细菌性青枯病是由青枯劳尔氏菌（Ralstonia solanacearum）（以下简称“青枯菌”）引起的一种典型的维管束病害，发病后严重影响作物产量与品质。选育抗病品种是从根本上解决青枯病危害的最有效措施，而了解植物免疫反应的分子机制是抗病育种的基础。越来越多的研究表明，维管束免疫具有细胞类型特异性。植物识别青枯菌后，通过信号传导诱导维管束细胞壁木质化，形成植物抵御青枯菌扩散的主要屏障。木质素的合成受到精细的调控，其关键合成酶基因在转录、翻译、时空特异性表达等不同方面受到调控。本文综述了植物对青枯菌的识别和信号传导机制，以及诱导维管束木质化调控青枯病抗性的研究进展，包括诱导木质素合成基因表达、木质素单体转运和聚合、不同木质素类型产生等分子机制，以期为利用维管束木质化改性技术进行青枯病的抗性育种提供理论依据。
关键词： 细菌性青枯病;  青枯劳尔氏菌;  木质化;  细胞壁;  诱导抗性    
Abstract:

Bacterial wilt, a typical vascular disease caused by Ralstonia solanacearum, is one of the most devastating diseases and dramatically reduces crop yield and quality. The most effective strategy for controlling wilt disease is breeding disease-resistant varieties, which requires understanding the molecular mechanisms underlying plant immune responses against R. solanacearum. However, more and more evidence suggests that vascular immune responses are cell type specific. After sensing of R. solanacearum, the cell wall lignification of vascular tissues plays a vital role in restricting the spread of R. solanacearum. Lignin biosynthesis pathway genes are strictly controlled at the transcriptional, translational, and spatial-temporal specific expression aspects. Here, we summarized the current understanding of the recognition and signal transductionupon R. solanacearum infection and the research progress of pathogen-induced vascular lignification on regulating resistance to R. solanacearum, including the expression of lignin biosynthesis genes, the transport and polymerization of monolignols, and the generation of different types of lignin. We hope that this review will provide a theoretical basis for breeding bacterial wilt disease-resistant cultivars by modifying vascular lignification.
Key words: bacterial wilt    Ralstonia solanacearum    lignification    cell wall    induced resistance
收稿日期: 2023-06-17 出版日期: 2023-11-03
CLC:  S432  
基金资助: 浙江省重点研发计划项目(2021C02009);国家自然科学基金项目(32270289)
通讯作者: 梁岩     E-mail: lichenying@zju.edu.cn;yanliang@zju.edu.cn
作者简介: 李陈莹（https://orcid.org/0009-0008-3817-9866），E-mail：lichenying@zju.edu.cn
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引用本文:

李陈莹,王冉,梁岩. 维管束木质化调控植物抗青枯病的研究进展[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(5): 633-643.

Chenying LI,Ran WANG,Yan LIANG. Research progress on the regulation of vascular lignification on defense against bacterial wilt of plants. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(5): 633-643.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2023.06.171    或    https://www.zjujournals.com/agr/CN/Y2023/V49/I5/633

图 1  木质素生物合成途径PAL：苯丙氨酸氨裂合酶；C4H：肉桂酸-4-羟化酶；4CL：4-香豆酸辅酶A连接酶；HCT：羟基肉桂酰基转移酶；C3H：对-香豆酸-3-羟化酶；CCoAOMT：咖啡酰辅酶A-O-甲基转移酶；CCR：肉桂酰辅酶A还原酶；CAD：肉桂醇脱氢酶；PD：被动扩散；ABC trans：ABC转运体；PCA：质子偶联反转运体。
图2  植物免疫信号分子调控木质素生物合成PAMPs：病原体相关分子模式；PRRs：模式识别受体；NLRs：核苷酸结合域并富含亮氨酸重复序列；ROS：活性氧；MAPK：丝裂原激活的蛋白激酶；SOD：超氧化物歧化酶。
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