菜缢管蚜的线粒体基因组及蚜亚科的系统发育分析

doi:10.3785/j.issn.1008-9209.2023.02.161

浙江大学学报（农业与生命科学版）

2024, Vol. 50

Issue (1): 53-64 DOI: 10.3785/j.issn.1008-9209.2023.02.161

植物保护

菜缢管蚜的线粒体基因组及蚜亚科的系统发育分析

林兴雨(

),宋南(

)

河南农业大学植物保护学院，河南郑州 450046

Mitochondrial genome of Lipaphis pseudobrassicae (Davis, 1914) and phylogenetic analysis of Aphidinae

Xingyu LIN(

),Nan SONG(

)

College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan, China

全文: PDF(1891 KB) HTML

摘要：

菜缢管蚜[Lipaphis pseudobrassicae (Davis, 1914)]是一种重要的农业害虫。本研究对菜缢管蚜的线粒体全基因组进行测序、分析，并基于线粒体基因组数据重建蚜亚科（Aphidinae）的系统发育关系。在系统发育分析中，内群包含蚜亚科的31个物种，外群选择绵蚜亚科（Eriosomatinae）中苹果绵蚜（Eriosoma lanigerum）、Paracolopha morrisoni和米倍蚜（Meitanaphis microgallis）3个物种，分别利用最大似然法和贝叶斯法重建蚜亚科的系统发育树。结果表明：菜缢管蚜的线粒体基因组全长16 743 bp（GenBank登录号为OP796483），包含37个基因（13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因）和1个非编码控制区。采用最大似然法和贝叶斯法构建的系统发育树均显示蚜族为单系群。在最大似然法分析结果中，长管蚜族为单系群，十字蚜属与短棒蚜属构成姐妹群。然而，在贝叶斯法分析结果中，长管蚜族为非单系群，十字蚜属与短棒蚜属+双尾蚜属亲缘关系较近。本研究为更好地理解长管蚜族、十字蚜属和菜缢管蚜的系统发育关系提供了线粒体基因组数据。

关键词： 半翅目; 蚜亚科; 菜缢管蚜; 线粒体基因组; 系统发育

Abstract:

Lipaphis pseudobrassicae (Davis, 1914) is an important agricultural insect pest. In this study, we sequenced and analyzed the entire mitochondrial genome of L. pseudobrassicae, and reconstructed the phylogenetic relationships of Aphidinae based on the mitochondrial genome data. In phylogenetic analyses, the ingroup included 31 species of Aphidinae, while the outgroup selected three species of Eriosomatinae (Eriosoma lanigerum, Paracolopha morrisoni and Meitanaphis microgallis). The maximum likelihood method and Bayesian method were used to reconstruct the phylogenetic tree of Aphidinae, respectively. The results showed that the mitochondrial genome of L. pseudobrassicae was 16 743 bp in length (GenBank accession No. OP796483), which contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and one non-coding control region. The phylogenetic tree constructed by the maximum likelihood method and Bayesian method both showed that the tribe Aphidini was a monophyletic group. The tribe Macrosiphini was recovered as a monophyletic group, and Lipaphis was a sister to Brevicoryne according to the maximum likelihood method. However, the tribe Macrosiphini was recovered as a non-monophyletic group, and Lipaphis was a sister to Brevicoryne+Diuraphis according to the Bayesian method. This study can enhance the understanding of the phylogenetic relationships among Macrosiphini, Lipaphis, and L. pseudobrassicae by utilizing mitochondrial genome data.

Key words: Hemiptera Aphidinae Lipaphis pseudobrassicae (Davis, 1914) mitochondrial genome phylogeny

收稿日期: 2023-02-16 出版日期: 2024-03-01

CLC:

S436.341.21

基金资助: 国家自然科学基金项目(U1904104)

通讯作者: 宋南 E-mail: xingyulin666666@163.com;songnan@henau.edu.cn

作者简介: 林兴雨（https://orcid.org/0000-0003-2368-6036），E-mail：xingyulin666666@163.com

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引用本文:

林兴雨,宋南. 菜缢管蚜的线粒体基因组及蚜亚科的系统发育分析[J]. 浙江大学学报（农业与生命科学版）, 2024, 50(1): 53-64.

Xingyu LIN,Nan SONG. Mitochondrial genome of Lipaphis pseudobrassicae (Davis, 1914) and phylogenetic analysis of Aphidinae. Journal of Zhejiang University (Agriculture and Life Sciences), 2024, 50(1): 53-64.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2023.02.161 或 https://www.zjujournals.com/agr/CN/Y2024/V50/I1/53

表1 用于构建系统发育树的物种信息

图1 菜缢管蚜线粒体基因组结构

表2 菜缢管蚜线粒体基因组注释

表3 菜缢管蚜线粒体基因组的核苷酸组成和偏斜

表4 菜缢管蚜线粒体基因组的同义密码子相对使用频率

表5 蚜亚科线粒体基因组的13个蛋白质编码基因的核苷酸多样性和进化率分析

图2 菜缢管蚜22个转运RNA基因的二级结构

图3 基于最大似然法构建的13个蛋白质编码基因核苷酸序列的系统发育树标尺表示进化距离。图4同。

图4 基于贝叶斯法构建的13个蛋白质编码基因氨基酸序列的系统发育树

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