马银花热激蛋白90基因家族的生物信息学及表达分析

doi:10.3785/j.issn.1008-9209.2022.08.012

浙江大学学报（农业与生命科学版）

2023, Vol. 49

Issue (5): 708-718 DOI: 10.3785/j.issn.1008-9209.2022.08.012

园艺科学

马银花热激蛋白90基因家族的生物信息学及表达分析

李丰延(

),瞿方茜,赵芳梦,王琪,周泓,张亮生,夏宜平(

),王秀云(

)

浙江大学农业与生物技术学院，观赏植物基因组与基因工程实验室，浙江杭州 310058

Bioinformatics and expression analyses of heat shock protein 90 gene family in Rhododendron ovatum

Fengyan LI(

),Fangqian QU,Fangmeng ZHAO,Qi WANG,Hong ZHOU,Liangsheng ZHANG,Yiping XIA(

),Xiuyun WANG(

)

Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang, China

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摘要：

为探究杜鹃花热激蛋白90（heat shock protein 90, Hsp90）在植物生长发育调节和高温胁迫响应中的作用，本研究以杜鹃花属中观赏价值高、环境适应性强的马银花（Rhododendron ovatum）为材料，利用生物信息学方法对其Hsp90家族开展全基因组水平鉴定以及基因结构、顺式作用元件、进化、表达模式等分析。结果表明马银花Hsp90家族有11个成员，分布在5条染色体上。启动子区顺式作用元件分析表明，Hsp90家族成员均参与植物激素代谢和逆境胁迫响应过程。利用马银花、拟南芥、番茄、茶、杜鹃、河南杜鹃、马缨杜鹃的Hsp90家族构建的系统进化树可被划分为4个主要分支。进化分析显示，Hsp90家族在杜鹃花属分化过程中受到纯化选择作用。Hsp90家族在不同组织器官中和高温处理下的表达模式表明，马银花Hsp90家族参与了花器官的发育和植物对高温胁迫的响应。本研究为杜鹃花Hsp90基因功能的深入解析奠定了基础。

关键词： 杜鹃花属; 热激蛋白90家族; 高温胁迫; 表达模式

Abstract:

To explore the roles of heat shock protein 90 (Hsp90) in plant growth development and response to high temperature stress, this study identified the Hsp90 family through bioinformatics methods in the whole genome of Rhododendron ovatum,which is a highly ornamental and widely adaptive species of Rhododendron. Gene structure, cis-acting elements, evolution, and expression pattern of the Hsp90 family were subsequently analyzed. The results showed that 11 members of the Hsp90 family were identified in R. ovatum, which were located on five chromosomes. Cis-acting element analysis showed that all the 11 members of the Hsp90 family were involved in the processes of plant hormone metabolism and stress responses. The phylogenetic tree of Hsp90 family in Arabidopsis thaliana, Solanum lycopersicum, Camellia sinensis, R. simsii, R. delavayi, R. henanense, and R. ovatum was constructed, within four main clades. Evolutionary analysis showed that the Hsp90 family has undergone purification selection during the differentiation of Rhododendron species. Moreover, the expression patterns of Hsp90 family in different tissues and under high temperature treatments indicated that Hsp90 family plays roles in flower development and the response of plants to high temperature stress. This study provides a foundation for further study of the functions of Hsp90 genes in Rhododendron.

Key words: Rhododendron heat shock protein 90 (Hsp90) family high temperature stress expression pattern

收稿日期: 2022-08-01 出版日期: 2023-11-03

CLC:

S685.21

基金资助: 国家自然科学基金项目(32171847);中央高校基本科研业务费专项资金项目(2021QNA6021)

通讯作者: 夏宜平,王秀云 E-mail: 3180100530@zju.edu.cn;ypxia@zju.edu.cn;xiuyunwang@zju.edu.cn

作者简介: 李丰延（https://orcid.org/0000-0003-1530-1861），E-mail：3180100530@zju.edu.cn

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引用本文:

李丰延,瞿方茜,赵芳梦,王琪,周泓,张亮生,夏宜平,王秀云. 马银花热激蛋白90基因家族的生物信息学及表达分析[J]. 浙江大学学报（农业与生命科学版）, 2023, 49(5): 708-718.

Fengyan LI,Fangqian QU,Fangmeng ZHAO,Qi WANG,Hong ZHOU,Liangsheng ZHANG,Yiping XIA,Xiuyun WANG. Bioinformatics and expression analyses of heat shock protein 90 gene family in Rhododendron ovatum. Journal of Zhejiang University (Agriculture and Life Sciences), 2023, 49(5): 708-718.

链接本文:

https://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2022.08.012 或 https://www.zjujournals.com/agr/CN/Y2023/V49/I5/708

表1 马银花Hsp90家族成员理化性质分析及亚细胞定位预测

图1 不同物种的Hsp90家族系统进化树

图2 拟南芥、杜鹃、茶、马银花 Hsp90 基因的物种间共线性分析蓝色线表示不同物种间Hsp90基因的共线性，灰色线表示其他基因的共线性。

图3 马银花Hsp90家族的物种内共线性分析红色线表示马银花物种内Hsp90基因的共线性，灰色线表示其他基因的共线性。

表2 马银花和杜鹃Hsp90同源基因对的Ka/Ks分析

图4 马银花Hsp90家族保守基序及基因结构

图5 马银花Hsp90家族顺式作用元件分析同一矩形内含多种颜色表示该位点可能涉及多种植物激素或逆境胁迫响应。

图6 马银花Hsp90基因家族的染色体定位

图7 马银花 Hsp90 基因在不同组织器官中的表达热图FPKM：每千个碱基的转录每百万映射读取的片段。

图8 不同温度处理下马银花 Hsp90 基因表达热图

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