盐胁迫培养下季也蒙毕赤酵母的转录组学差异分析

doi:10.3785/j.issn.1008-9209.2019.09.091

浙江大学学报（农业与生命科学版）

2020, Vol. 46

Issue (4): 400-406 DOI: 10.3785/j.issn.1008-9209.2019.09.091

生物科学与技术

盐胁迫培养下季也蒙毕赤酵母的转录组学差异分析

曹璇(

),郑晓冬(

)

浙江大学生物系统工程与食品科学学院，杭州 310058

Transcriptomic difference analysis of Meyerozyma guilliermondii in response to salt stress

Xuan CAO(

),Xiaodong ZHENG(

)

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310027, China

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摘要：

在本实验室从海南省西沙群岛海沙里分离出1株季也蒙毕赤酵母（Meyerozyma guilliermondii），并证实该酵母菌株有较好的耐盐性，在12% NaCl胁迫培养条件下仍可生长的基础上，本文通过Illumina HiSeq^TM高通量测序技术对经盐胁迫培养与非盐胁迫培养24 h的M. guilliermondii进行转录组测序比较。结果表明：2组样品共有 1 027个显著性差异表达基因，其中458个基因表达上调，569个基因表达下调。通过基因本体（gene ontology, GO）功能注释发现，经盐胁迫处理后M. guilliermondii的差异表达基因主要富集在生物过程分类中，其中核苷酸代谢、糖代谢及辅酶代谢基因产生较大差异。对差异基因进行京都基因与基因组百科全书数据库（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路富集分析，发现大部分富集通路都与细胞分裂和代谢有关，与GO富集结果相对应。上述结果可以为盐胁迫培养对M. guilliermondii的生长影响及进一步的生物学探究提供科学依据。

关键词： 季也蒙毕赤酵母; 盐胁迫; 转录组学; 高通量测序

Abstract:

Previous work proved that a strain of Meyerozyma guilliermondii isolated from the Xisha islands of Hainan Province had good salt-tolerant ability which could grow under 12% NaCl stress culture condition. Based on the above results, the transcriptome sequencing of the straincultured under salt stress and non-salt stress for 24 h was constructed by Illumina HiSeq^TM in this study. The results were as follows: the two samples yielded 1 027 significantly differential expression genes, of which 458 genes were up-regulated and 569 genes were down-regulated. According to the gene ontology (GO) functional annotations, the differential expression genes of M. guilliermondii treated with salt stress were mainly concentrated on the classification of biological process, in which nucleotide metabolism, sugar metabolism and coenzyme metabolism genes were greatly different. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the differential expression genes showed that the most of the enrichment pathways were related to cell division and metabolism, which were corresponding to the GO enrichment results. The above results can provide scientific basis for further biological research on the effect of the growth of M. guilliermondii under salt stress.

Key words: Meyerozyma guilliermondii salt stress transcriptomics high-throughput sequencing

收稿日期: 2019-09-09 出版日期: 2020-09-11

CLC:

Q 939.9

基金资助: 国家自然科学基金(31571897);“十三五”国家重点研发计划(2016TFD0401201)

通讯作者: 郑晓冬 E-mail: 452353248@qq.com;xdzheng@zju.edu.cn

作者简介: 曹璇（https://orcid.org/0000-0003-4271-2500），E-mail：452353248@qq.com

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引用本文:

曹璇,郑晓冬. 盐胁迫培养下季也蒙毕赤酵母的转录组学差异分析[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(4): 400-406.

Xuan CAO,Xiaodong ZHENG. Transcriptomic difference analysis of Meyerozyma guilliermondii in response to salt stress. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(4): 400-406.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2019.09.091 或 http://www.zjujournals.com/agr/CN/Y2020/V46/I4/400

表1 实时荧光定量反转录PCR扩增目的基因的引物序列

表2 数据产出质量情况统计

图1 试验组差异表达基因火山图FC：差异倍数；FDR：校正后的P值。

图2 差异表达基因GO富集柱状图

图3 差异表达基因的KEGG通路富集散点图

图4 基于实时荧光定量反转录PCR（RT-qPCR）的季也蒙毕赤酵母基因转录表达分析FC：差异倍数。

1	YAN Y, ZHANG X Y, ZHENG X F, et al. Control of postharvest blue mold decay in pears by Meyerozyma guilliermondii and its effects on the protein expression profile of pears. Postharvest Biology and Technology, 2018,136:124-131. DOI:10.1016/j.postharvbio.2017.10.016 doi: 10.1016/j.postharvbio.2017.10.016
2	高云慨,张荣意,钟利文,等.1株新分离拮抗酵母菌株对杧果炭疽病生防效果及其分类鉴定.热带生物学报,2015,6(1):47-52. GAO Y K, ZHANG R Y, ZHONG L W, et al. Classification and identification of a new isolated yeast strain and its biocontrol activity against Anthracnose of mango fruit. Journal of Tropical Biology, 2015,6(1):47-52. (in Chinese with English abstract)
3	丁玥,孟帆,王奎,等.季也蒙毕赤酵母对采后草莓病害控制及冷藏品质的影响.南京农业大学学报,2010,33(4):64-68. DING Y, MENG F, WANG K, et al. Effects of Pichia guilliermondii on disease control and quality of postharvest strawberry during cold storage. Journal of Nanjing Agricultural University, 2010,33(4):64-68. (in Chinese with English abstract)
4	RANGARAJAN S, SALEENA L M, VASUDEVAN P, et al. Biological suppression of rice diseases by Pseudomonas spp. under saline soil conditions. Plant and Soil, 2003,251(1):73-82.
5	SIM C S F, CHEOW Y L, NG S L, et al. Biocontrol activities of metal-tolerant endophytes against Ganoderma boninense in oil palm seedlings cultivated under metal stress. Biological Control, 2019,132:66-71. DOI:10.1016/j.biocontrol.2019.02.001 doi: 10.1016/j.biocontrol.2019.02.001
6	SHARMA A, KASHYAP P L, SRIVASTAVA A K, et al. Isolation and characterization of halotolerant bacilli from chickpea (Cicer arietinum L.) rhizosphere for plant growth promotion and biocontrol traits. European Journal of Plant Pathology, 2019,153(3):787-800. DOI:10.1007/s10658-018-1592-7 doi: 10.1007/s10658-018-1592-7
7	WANG Z, GERSTEIN M, SNYDER M. RNA-Seq: a revolutionary tool for transcriptomics. Nature Reviews Genetics, 2009,10(1):57-63. DOI:10.1038/nrg2484 doi: 10.1038/nrg2484
8	高弢,史建荣.基于高通量测序技术分析麝香草酚处理禾谷镰孢菌后转录组学的变化.江苏农业学报,2017,33(6):1257-1265. DOI:10.3969/j.issn.1000-4440.2017.01.009 GAO T, SHI J R. Transcriptome analysis of Fusarium graminearum treated with thymol based on high-throughput sequencing technology. Jiangsu Journal of Agricultural Sciences, 2017,33(6):1257-1265. (in Chinese with English abstract) doi: 10.3969/j.issn.1000-4440.2017.01.009
9	鲍林飞,王新星,何健瑜,等.基于Illumina平台的厚壳贻贝外套膜转录组从头测序.浙江大学学报(农业与生命科学版),2015,41(4):394-406. DOI:10.3785/j.issn.1008-9209.2014.12.291 BAO L F, WANG X X, HE J Y, et al. Illumina-based transcriptome sequencing of mussel Mytiluscoruscus mantle. Journal of Zhejiang University (Agriculture and Life Sciences), 2015,41(4):394-406. (in Chinese with English abstract) doi: 10.3785/j.issn.1008-9209.2014.12.291
10	宋雪飞,郭晶晶,姜静,等.植物乳杆菌FS5-5在盐胁迫下的转录组学分析.食品科学,2017,38(6):20-26. DOI:10.7506/spkx1002-6630-201706004 SONG X F, GUO J J, JIANG J, et al. Transcriptomic analyses of Lactobacillus plantarum FS5-5 against salt stress. Food Science, 2017,38(6):20-26. (in Chinese with English abstract) doi: 10.7506/spkx1002-6630-201706004
11	RIO D C, ARES M J, HANNON G J, et al. Purification of RNA using TRIzol (TRI reagent). Cold Spring Harbor Protocols, 2010(6):pdb.prot5439. DOI:10.1101/pdb.prot5439 doi: 10.1101/pdb.prot5439
12	GRABHERR M G, HAAS B J, YASSOUR M, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 2011,29(7):130-644. DOI:10.1038/nbt.1883 doi: 10.1038/nbt.1883
13	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2－△△CT method. Methods, 2001,25(4):402-408. DOI:10.1006/meth.2001.1262 doi: 10.1006/meth.2001.1262
14	YOUNG M D, WAKEFIELD M J, SMYTH G K, et al. Gene ontology analysis for RNA-Seq: accounting for selection bias. Genome Biology, 2010,11(2):R14. DOI:10.1186/gb-2010-11-2-r14 doi: 10.1186/gb-2010-11-2-r14
15	KANEHISA M, ARAKI M, GOTO S, et al. KEGG for linking genomes to life and the environment. Nucleic Acids Research, 2008,36(Suppl.Ⅰ):D480-D484. DOI:10.1093/nar/gkm882 doi: 10.1093/nar/gkm882
16	QI Q, GUO Z L, LIANG Y L, et al. Hydrogen sulfide alleviates oxidative damage under excess nitrate stress through MAPK/NO signaling in cucumber. Plant Physiology and Biochemistry, 2019,135:1-8. DOI:10.1016/j.plaphy.2018.11.017 doi: 10.1016/j.plaphy.2018.11.017
17	MANSURI R M, SHOBBAR Z, JELODAR N B, et al. Dissecting molecular mechanisms underlying salt tolerance in rice: a comparative transcriptional profiling of the contrasting genotypes. Rice, 2019,12:13. DOI:10.1186/s12284-019-0273-2 doi: 10.1186/s12284-019-0273-2
18	SHEN L K, ZHUANG B C, WU Q, et al. Phosphatidic acid promotes the activation and plasma membrane localization of MKK7 and MKK9 in response to salt stress. Plant Science: An International Journal of Experimental Plant Biology, 2019,287:110190. DOI:10.1016/j.plantsci.2019.110190 doi: 10.1016/j.plantsci.2019.110190
19	DING H N, MA D Y, HUANG X, et al. Exogenous hydrogen sulfide alleviates salt stress by improving antioxidant defenses and the salt overly sensitive pathway in wheat seedlings. Acta Physiologiae Plantarum, 2019,41:123. DOI:10.1007/s11738-019-2918-6 doi: 10.1007/s11738-019-2918-6

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