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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2020, Vol. 46 Issue (2): 209-216    DOI: 10.3785/j.issn.1008-9209.2019.05.061
资源利用与环境保护     
伴生盐生植物脱盐效应对胡杨幼苗生理特性的影响
文强1,2,3(),韩炜1,2,3(),管文轲3,武胜利1,2,3,孔凯凯1,2,3
1.新疆师范大学地理科学与旅游学院，乌鲁木齐 830054
2.新疆干旱区湖泊环境与资源重点实验室，乌鲁木齐 830054
3.新疆林业科学院林业科技推广处，乌鲁木齐 830000
Effects of desalination of associated halophytes on physiological characteristics of Populus euphratica seedlings
Qiang WEN1,2,3(),Wei HAN1,2,3(),Wenke GUAN3,Shengli WU1,2,3,Kaikai KONG1,2,3
1.College of Geographical Science and Tourism, Xinjiang Normal University, Urumqi 830054, China
2.Key Laboratory of Lake Environment and Resources in Xinjiang Arid Region, Urumqi 830054, China
3.Forestry Science and Technology Promotion Office, Xinjiang Academy of Forestry Sciences, Urumqi 830000, China
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摘要：

为了筛选适用于胡杨幼苗最佳脱盐效果的盐生植物，以一年生胡杨幼苗为试验材料，采用盆栽控盐胁迫的方法，选取新疆常见盐生植物碱蓬（T1）、盐角草（T2）和盐节木（T3）作为伴生植物进行脱盐试验，设置5 g/L NaCl（B）和11 g/L NaCl（C）2个盐分处理，以及1个平行处理对照（CK0：0 g/L NaCl+胡杨）和3个各区组对照（CK1：0 g/L NaCl+碱蓬；CK2：0 g/L NaCl+盐角草；CK3：0 g/L NaCl+盐节木），通过测定胡杨幼苗叶片生理生化指标探讨脱盐作用对其影响规律。结果表明：伴生盐生植物对土壤具有明显的脱盐效果（P＜0.05），在5 g/L NaCl（B）条件下，T1脱盐率为26.8%，T2脱盐率为22.0%，T3脱盐率为14.2%，在11 g/L NaCl（C）条件下，T1脱盐率为14.9%，T2脱盐率为23.1%，T3脱盐率为19.7%。胡杨幼苗叶片脯氨酸（proline, Pro）与丙二醛（malondialdehyde, MDA）含量较各对照均总体呈上升趋势，但增加均不明显。在同一B盐分条件下，不同盐生植物处理的胡杨Pro含量变化为T3＞T2＞CK0＞T1＞CK3＞CK2＞CK1，MDA含量变化为T3＞CK0＞CK3＞T2＞T1＞CK2＞CK1；在同一C盐分条件下，不同盐生植物处理的胡杨Pro含量变化为T3＞T1＞T2＞CK0＞CK3＞CK2＞CK1，MDA含量变化为T3＞T1＞T2＞CK0＞CK3＞CK2＞CK1。抗氧化酶中超氧化物歧化酶和过氧化物酶活性与MDA含量呈线性相反的趋势。综合各项生理生态指标可见，3种伴生盐生植物在30 d内产生了较为显著的脱盐效果，其中：在5 g/L NaCl条件下，伴生碱蓬对胡杨幼苗脱盐效果最佳；在11 g/L NaCl条件下，伴生盐角草脱盐效果最佳。
关键词： 胡杨;  盐生植物;  脱盐作用;  渗透物质    
Abstract:

To select suitable halophytes for best desalting effect of Populus euphratica seedlings, taking one-year P. euphratica seedlings as the experimental materials, and Suaeda glauca (Bunge)Bunge (T1), Salicornia europaea L. (T2) and Halocnemum strobilaceum (Pall.) M. Bieb.(T3) of the common halophytes in Xinjiang as the associated plants, the seedlings of three P. euphratica and one associated plant were cultivated in pots treated with NaC1 solutions of 5 g/L (B) and 11 g/L (C) and three replications, and a parallel treatment and three block treatments were set as the controls (CK0: 0 g/L NaCl+P. euphratica; CK1: 0 g/L NaCl+S. glauca; CK2: 0 g/L NaCl+S. europaea; CK3: 0 g/L NaCl+H. strobilaceum), and then the physiological indexes of P. euphratica seedlings were determinated to explore the rule of desalting effect on it. The results showed that the associated halophytes had obvious desalting effect on soil (P＜0.05). Under the condition of 5 g/L NaC1 (B), the desalination rate of T1, T2 and T3 was 26.8%, 22.0% and 14.2%, respectively. Under the condition of 11 g/L NaC1 (C), the desalination rate of T1, T2 and T3 was 14.9%, 23.1% and 19.7%, respectively. The contents of proline (Pro) and malondialdehyde (MDA) in P. euphratica seedlings increased on the whole as compared with the controls, but the increase was not obvious. Under the same B salt concentration, the changes of Pro and MDA contents under the different treatments were as follows: T3＞T2＞CK0＞T1＞CK3＞CK2＞CK1, and T3＞CK0＞CK3＞T2＞T1＞CK2＞CK1, respectively. Under the same C salt concentration, the changes of Pro and MDA contents under the different treatments were as follows: T3＞T1＞T2＞CK0＞CK3＞CK2＞CK1, and T3＞T1＞T2＞CK0＞CK3＞CK2＞CK1, respectively. Antioxidant activities of the superoxide dismutase (SOD) and peroxidase (POD) were linearly opposite to MDA content. In a word, based on the physiological and ecological indicators, the three associated halophytes produce a significant desalting effect in the 30 d. Under the condition of 5 g/L NaC1, the desalting effect of S. glauca on P. euphratica seedlings is the best; under the condition of 11 g/L NaC1, the desalting effect of S. europaea is the best.
Key words: Populus euphratica    halophyte    desalination    osmotic substance
收稿日期: 2019-05-06 出版日期: 2020-05-22
CLC:  S 792.119  
基金资助: 新疆维吾尔自治区科技支撑专项“塔里木河中下游胡杨生态修复研究与示范”(HY-2.3);新疆师范大学“十三五”校级重点学科（地理学）招标课题项目(17SDKD0704)
通讯作者: 韩炜     E-mail: 2927289045@qq.com;hanweiaa@163.com
作者简介: 文强（https://orcid.org/0000-0002-4812-3929），E-mail：2927289045@qq.com
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文强,韩炜,管文轲,武胜利,孔凯凯. 伴生盐生植物脱盐效应对胡杨幼苗生理特性的影响[J]. 浙江大学学报（农业与生命科学版）, 2020, 46(2): 209-216.

Qiang WEN,Wei HAN,Wenke GUAN,Shengli WU,Kaikai KONG. Effects of desalination of associated halophytes on physiological characteristics of Populus euphratica seedlings. Journal of Zhejiang University (Agriculture and Life Sciences), 2020, 46(2): 209-216.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2019.05.061    或    http://www.zjujournals.com/agr/CN/Y2020/V46/I2/209

处理 TreatmentCK0CK1T1BT1CCK2T2BT2CCK3T3BT3C
ECp/(mS/m)268e268e298d328a268e296d307c268e302c314b
表1  各处理土壤的电导率
图1  伴生盐生植物对土壤盐分（电导率）的影响各处理符号表示的含义详见表1注。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
图2  伴生盐生植物脱盐作用对胡杨幼苗Pro含量的影响各处理符号表示的含义详见表1注。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
图3  伴生盐生植物脱盐作用对胡杨幼苗MDA含量的影响各处理符号表示的含义详见表1注。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
图4  伴生盐生植物脱盐作用对胡杨幼苗SOD和POD活性的影响各处理符号表示的含义详见表1注。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
1 MUNNS R. Physiological processes limiting plant growth in saline soil: some dogmas and hypotheses. Plant, Cell & Environment, 2010,16(1):15-24. DOI:10.1111/j.1365-3040.1993.tb00840.x
doi: 10.1111/j.1365-3040
2 张有福,陈拓,费贯清,等.盐度对三种荒漠植物渗透调节物质积累影响的研究.中国沙漠,2007,27(5):787-790.
ZHANG Y F, CHEN T, FEI G Q, et al. Effects of salinity on the contents of osmotica of three desert plants. Journal of Desert Research, 2007,27(5):787-790. (in Chinese with English abstract)
3 陈亚宁,崔旺诚,李卫红,等.塔里木河的水资源利用与生态保护.地理学报, 2003,58(2):215-222.
CHEN Y N, CUI W C, LI W H, et al. Utilization of water resources and ecological protection in the Tarim River. Acta Geographica Sinica, 2003,58(2):215-222. (in Chinese with English abstract)
4 王东健,陈其凌,李铭,等.胡杨不同生长阶段的耐盐性.新疆林业,1998,12(4):9-10.
WANG D J, CHEN Q L, LI M, et al. Salt tolerance of Populus euphratica at different growth stages. Forestry of Xinjiang, 1998,12(4):9-10. (in Chinese)
5 鲁艳,雷加强,曾凡江,等.NaCl处理对胡杨生长及生理生态特征的影响.干旱区研究,2015,32(2):279-285. DOI:10.13866/j.azr.2015.02.10
LU Y, LEI J Q, ZENG F J, et al. Effects of NaCl treatments on growth and ecophysiological characteristics of Populus euphratica. Arid Zone Research, 2015,32(2):279-285. (in Chinese with English abstract)
doi: 10.13866/j.azr.2015.02.10
6 何新林,陈书飞,王振华,等.咸水灌溉对沙土土壤盐分和胡杨生理生长的影响.生态学报,2012,32(11):3449-3459. DOI:10.5846/stxb201104280562
HE X L, CHEN S F, WANG Z H, et al. Effect of saline water irrigation on sand soil salt and the physiology and growth of Populus euphratica Oliv. Acta Ecologica Sinica, 2012,32(11):3449-3459. (in Chinese with English abstract)
doi: 10.5846/stxb201104280562
7 李菊艳,赵成义,闫映宇,等.盐分对胡杨(Populus euphratica)幼苗生长和离子平衡的影响.干旱区地理,2016,39(3):613-620. DOI:10.13826/j.cnki.cn65-1103/x.2016.03.019
LI J Y, ZHAO C Y, YAN Y Y, et al. Effect of salinity on growth, ionic homeostasis in organs of Populus euphratica seedlings. Arid Land Geography, 2016,39(3):613-620. (in Chinese with English abstract)
doi: 10.13826/j.cnki.cn65-1103/x.2016.03.019
8 朱会娟,王瑞刚,陈少良,等.NaCl胁迫下胡杨(Populus euphratica)和群众杨(P. popularis)抗氧化能力及耐盐性.生态学报,2007,27(10):4113-4121.
ZHU H J, WANG R G, CHEN S L, et al. Genotypic differences between Populus euphratica and P. popularis in antioxidative ability and salt tolerance under NaCl stress. Acta Ecologica Sinica, 2007,27(10):4113-4121. (in Chinese with English abstract)
9 史军辉,王新英,刘茂秀,等.NaCl胁迫对胡杨幼苗叶主要渗透调节物质的影响.西北林学院学报,2014,29(6):6-11. DOI:10.3969/j.issn.1001-7461.2014.06.02
SHI J H, WANG X Y, LIU M X, et al. Effect of NaCl stress on main osmotic adjustment substances in the seedling leaves of Populus euphratica. Journal of Northwest Forestry University, 2014,29(6):6-11. (in Chinese with English abstract)
doi: 10.3969/j.issn.1001-7461.2014.06.02
10 杨升,张华新,张丽.植物耐盐生理生化指标及耐盐植物筛选综述.西北林学院学报,2010,25(3):59-65.
YANG S, ZHANG H X, ZHANG L. Physiological and biochemical indices of salt tolerance and scanning of salt-tolerance plants: a review. Journal of Northwest Forestry University, 2010,25(3):59-65. (in Chinese with English abstract)
11 潘书轩,孙良斌,张少民,等.盐地碱蓬在北疆的生育规律及其对盐渍土改良效果.西北农业学报,2014,23(12):201-206. DOI:10.7606/j.issn.1004-1389.2014.12.031
PAN S X, SUN L B, ZHANG S M, et al. Growth regularity of Suaeda salsa L. and its effects on salt soil improvement in the northern Xinjiang. Acta Agriculturae Boreali-Occidentalis Sinica, 2014,23(12):201-206. (in Chinese with English abstract)
doi: 10.7606/j.issn.1004-1389.2014.12.031
12 祁通,孙阳讯,黄建,等.两种盐生植物在南北疆地区的适生性及吸盐能力.中国土壤与肥料,2017,21(1):144-148. DOI:10.11838/sfsc.20170124
QI T, SUN Y X, HUANG J, et al. The adaptability and salt absorption ability of two kinds of halophyte in southern and northern of Xinjiang. Soil and Fertilizer Sciences in China, 2017,21(1):144-148. (in Chinese with English abstract)
doi: 10.11838/sfsc.20170124
13 肖克飚.宁夏银北地区耐盐植物改良盐碱土机理及试验研究.陕西,杨凌:西北农林科技大学,2013.
XIAO K B. Mechanism and experimental study on improvement of saline-alkali soil by salt-tolerant plants in Yinbei area, Ningxia. Yangling, Shaanxi: Northwest A & F University, 2013. (in Chinese with English abstract)
14 赵振勇,张科,王雷,等.盐生植物对重盐渍土脱盐效果.中国沙漠,2013,33(5):1420-1425. DOI:10.7522/j.issn.1000-694X.2013.00207
ZHAO Z Y, ZHANG K, WANG L, et al. Desalination effect of halophytes in heavily salinized soil of Karamay. Journal of Desert Research, 2013,33(5):1420-1425. (in Chinese with English abstract)
doi: 10.7522/j.issn.1000-694X.2013.00207
15 郗金标,田长彦,阎平,等.新疆盐生植物区系初探.中国生态农业学报,2006,14(1):7-10.
XI J B, TIAN C Y, YAN P, et al. Primary research on the halophyte flora in Xinjiang. Chinese Journal of Eco-Agriculture, 2006,14(1):7-10. (in Chinese with English abstract)
16 高俊凤.植物生理学实验指导.北京:高等教育出版社,2006.
GAO J F. Plant Physiology Experiment Guide. Beijing: Higher Education Press, 2006. (in Chinese)
17 李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000.
LI H S. Principles and Techniques of Plant Physiological and Biochemical Experiments. Beijing: Higher Education Press, 2000. (in Chinese)
18 张蛟,崔士友,冯芝祥.种植碱蓬和秸秆覆盖对沿海滩涂极重度盐土盐分动态与脱盐效果的影响.应用生态学报,2018,29(5):1686-1694. DOI:10.13287/j.1001-9332.201805.034
ZHANG J, CUI S Y, FENG Z X. Effects of Suaeda glauca planting and straw mulching on soil salinity dynamics and desalination in extremely heavy saline soil of coastal areas. Chinese Journal of Applied Ecology, 2018,29(5):1686-1694. (in Chinese with English abstract)
doi: 10.13287/j.1001-9332.201805.034
19 张永宏.盐碱地种植耐盐植物的脱盐效果.甘肃农业科技,2005(3):48-49.
ZHANG Y H. Desalination effects of salttolerant plants growing in alkali-saline soil in Ningxia. Gansu Agricultural Science and Technology, 2005(3):48-49. (in Chinese with English abstract)
20 RAHDARI P, TAVAKOLI S, HOSSEINI S M. Studying of salinity stress effect on germination, proline, sugar, protein, lipid and chlorophyll content in purslane (Portulaca oleracea L.) leaves. Journal of Stress Physiology & Biochemistry, 2012,8(1:182-193.
21 孙聪聪,赵海燕,郑彩霞.NaCl胁迫对银杏幼树渗透调节物质及脯氨酸代谢的影响.植物生理学报,2017,53(3):470-476. DOI:10.13592/j.cnki.ppj.2017.0057
SUN C C, ZHAO H Y, ZHENG C X. Effects of NaCl stress on osmolyte and proline metabolism in Ginkgo biloba seedling. Plant Physiology Journal, 2017,53(3):470-476. (in Chinese with English abstract)
doi: 10.13592/j.cnki.ppj.2017.0057
22 柳福智,羊健麟.盐胁迫对甘草愈伤组织渗透调节的影响.植物生理学报,2015,51(7):1038-1044. DOI:10.13592/j.cnki.ppj.2015.0111
LIU F Z, YANG J L. Effect of salt stress on osmotic adjustment of Glycyrrhiza callus. Plant Physiology Journal, 2015,51(7):1038-1044. (in Chinese with English abstract)
doi: 10.13592/j.cnki.ppj.2015.0111
23 REGINATO M A, CASTAGNA A, FURLAN A, et al. Physiological responses of a halophytic shrub to salt stress by Na2SO4 and NaCl: oxidative damage and the role of polyphenols in antioxidant orotection. AoB Plants, 2016,6(4):plu042. DOI:10.1093/aobpla/plu042
doi: 10.1093/aobpla/plu042
24 FARHANGI-ABRIZ S, TORABIAN S. Antioxidant enzyme and osmotic adjustment changes in bean seedlings as affected by biochar under salt stress. Ecotoxicology and Environmental Safety, 2017,137:64-70. DOI:10.1016/j.ecoenv.2016.11.029
doi: 10.1016/j.ecoenv.2016.11.029
25 TOMMASINO E, GRIFFA S, GRUNBERG K A, et al. Malondialdehyde content as a potential biochemical indicator of tolerance Cenchrus ciliaris L. genotypes under heat stress treatment. Grass and Forage Science, 2012,67(3):456-459. DOI:10.1111/j.1365-2494.2012.00851.x
doi: 10.1111/j.1365-2494.2012.00851.x
26 郑春芳,冀德伟,刘伟成,等.NaCl胁迫下高纬度移植桐花树幼苗的生理生态效应.应用生态学报,2011,22(9):2279-2284. DOI:10.13287/j.1001-9332.2011.0321
ZHEN C F, JI D W, LIU W C, et al. Eco-physiological responses of high-latitude transplanted Aegiceras corniculatum seedlings to NaCl stress. Chinese Journal of Applied Ecology, 2011,22(9):2279-2284. (in Chinese with English abstract)
doi: 10.13287/j.1001-9332.2011.0321
27 贾漫丽,李娜,李季生,等.盐胁迫对4个桑树品种生理生化特性的影响.西北林学院学报,2016,31(5):96-101. DOI:10.3969/j.issn.1001-7461.2016.05.16
JIA M L, LI N, LI J S, et al. Effects of salt stress on physiological and biochemical characteristics of 4 mulberry varieties. Journal of Northwest Forestry University, 2016,31(5):96-101. (in Chinese with English abstract)
doi: 10.3969/j.issn.1001-7461.2016.05.16
28 GOSSETT D R, BANKS S W, MILLHOLLON E, et al. Antioxidant response to NaCl stress in a control and NaCl-tolerant cotton cell line grown in the presence of paraquat, buthionine sulfoximine and exogenous glutathione. Plant Physiology, 1996,112:803-809.
29 张雯莉,刘玉冰,刘立超.盐胁迫对枸杞(Lycium barbarum)叶片生理的影响.中国沙漠,2018,38(2):294-299. DOI:10.7522/j.issn.1000-694X.2017.00107
ZHANG W L, LIU Y B, LIU L C. Effect of single and mixed salt stress on leaf physiological characteristics of Lycium barbarum. Journal of Desert Research, 2018,38(2):294-299. (in Chinese with English abstract)
doi: 10.7522/j.issn.1000-694X.2017.00107
30 杨国会,石德成.NaCl胁迫对甘草叶片相对含水量及保护酶活性的影响.河南农业科学,2009(12):104-106. DOI:10.3969/j.issn.1004-3268.2009.12.032
YANG G H, SHI D C. Effects of NaCl stress on relative water content and defense enzymes activities in leaves of Glycyrrhiza uralensis Fisch. Journal of Henan Agricultural Sciences, 2009(12):104-106. (in Chinese with English abstract)
doi: 10.3969/j.issn.1004-3268.2009.12.032
31 包灵,黄俊华,杨文英,等.NaCl胁迫对盐角草不同阶段生长和水分生理的影响.山东农业科学,2017,49(6):48-53. DOI:10.14083/j.issn.1001-4942.2017.06.010
BAO L, HUANG J H, YANG W Y, et al. Effects of NaCl stress on growth and water physiology at different stages of Salicornia europaea. Shandong Agricultural Sciences, 2017,49(6):48-53. (in Chinese with English abstract)
doi: 10.14083/j.issn.1001-4942.2017.06.010
32 张立华,陈小兵.盐碱地柽柳“盐岛”和“肥岛”效应及其碳氮磷生态化学计量学特征.应用生态学报,2015,26(3):653-658.
ZHANG L H, CHEN X B. Characteristics of ‘salt island’ and ‘fertile island’ for Tamarix chinensis and soil carbon, nitrogen and phosphorus ecological stoichiometry in saline-alkali land. Chinese Journal of Applied Ecology, 2015,26(3):653-658. (in Chinese with English abstract)
33 尹传华,董积忠,石秋梅,等.不同生境下盐生灌木盐岛效应的变化及生态学意义.土壤学报,2012,49(2):289-295.
YIN C H, DONG J Z, SHI Q M, et al. Salt island effect of halophytic shrubs in different habitats and its ecological implication. Acta Pedologica Sinica, 2012,49(2):289-295. (in Chinese with English abstract)
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