不同茶树品种的茶树花黄酮苷研究

doi:10.3785/j.issn.1008-9209.2019.03.111

浙江大学学报（农业与生命科学版）

2019, Vol. 45

Issue (6): 707-714 DOI: 10.3785/j.issn.1008-9209.2019.03.111

食品科学

不同茶树品种的茶树花黄酮苷研究

李金(

),陈莲芙,金恩惠,李博,屠幼英(

)

浙江大学农业与生物技术学院茶学系，杭州 310058

Research on flavonoid glycosides of tea flower in different tea plant cultivars

Jin LI(

),Lianfu CHEN,Eunhye KIM,Bo LI,Youying TU(

)

Department of Tea Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

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摘要：

利用超高效液相色谱-质谱联用（ultra-high performance liquid chromatography-mass spectrometry, UHPLC-MS）技术检测出梅占、水古、黄叶早、政和大白、福建水仙5个不同茶树品种茶树花的12种黄酮苷，分别为杨梅素-3-O-芸香糖苷、杨梅素-3-O-半乳糖苷、杨梅素-3-O-葡萄糖苷、槲皮素-3-O-芸香糖半乳糖苷、槲皮素-3-O-芸香糖葡萄糖苷、山柰酚-3-O-芸香糖半乳糖苷、槲皮素-3-O-半乳糖苷、槲皮素-3-O-葡萄糖苷、山柰酚-3-O-芸香糖葡萄糖苷、山柰酚-3-O-半乳糖苷、山柰酚-3-O-芸香糖苷、山柰酚-3-O-葡萄糖苷。通过比较不同品种间各黄酮苷的含量，发现茶树花中含量最高的2种黄酮苷为山柰酚-3-O-芸香糖葡萄糖苷和山柰酚-3-O-芸香糖半乳糖苷，梅占茶树花中山柰酚-3-O-芸香糖葡萄糖苷与黄叶早茶树花中山柰酚-3-O-芸香糖半乳糖苷的质量分数分别为1.208 mg/g和0.962 mg/g，显著高于其他品种；政和大白、黄叶早和水古茶品种茶树花中槲皮素-3-O-芸香糖半乳糖苷的含量显著高于其他品种。在5个茶树品种中，福建水仙品种茶树花中槲皮素-3-O-芸香糖葡萄糖苷的含量最高，且显著高于政和大白、梅占和黄叶早；福建水仙茶树花中的杨梅素-3-O-葡萄糖苷含量比其他品种中的高出近4倍，而其杨梅素-3-O-半乳糖苷的含量只有其他品种的20%左右；政和大白茶树花的黄酮苷总量在5个品种中最低。尽管各茶树花黄酮苷组分在不同品种中的含量呈现明显的差异性，但在所有品种的茶树花中均表现为其山柰酚苷含量＞槲皮素苷含量＞杨梅素苷含量，且主要以三糖基黄酮苷的形式存在。

关键词： 茶树花; 黄酮苷; 超高效液相色谱-质谱联用; 茶树品种

Abstract:

Twelve different flavonoid glycosides were identified and quantified by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method from tea flower of five different tea plant cultivars (Meizhan, Shuigu, Huangyezao, Zhenghe Dabai, and Fujian Shuixian), which were myricetin-3-O-rutinoside (Myr-rut), myricetin-3-O-galactoside (Myr-gala), myricetin-3-O-glucoside (Myr-glu), quercetin-3-O-rutinoside-galactoside (Que-rut-gala), quercetin-3-O-rutinoside-glucoside (Que-rut-glu), kaempferol-3-O-rutinoside-galactoside (Kae-rut-gala), quercetin-3-O-galactoside (Que-gala), quercetin-3-O-glucoside (Que-glu), kaempferol-3-O-rutinoside-glucoside (Kae-rut-glu), kaempferol-3-O-galactoside (Kae-gala), kaempferol-3-O-rutinoside (Kae-rut）, kaempferol-3-O-glucoside (Kae-glu）.The contents of Kae-rut-glu and Kae-rut-gala were significantly higher than other flavonoid glycosides in tea flower, which were up to 1.208 mg/g in Meizhan and 0.962 mg/g in Huangyezao, respectively. The content of Que-rut-gala in tea flower of Zhenghe Dabai, Huangyezao, and Shuigu were significantly higher than that in others; the content of Que-rut-glu in tea flower of Fujian Shuixian was the highest among the five tea plant cultivars, which was significantly higher than that of Zhenghe Dabai, Meizhan, and Huangyezao; the amount of Myr-glu in Fujian Shuixian tea flower was four times higher than that of in others; however, its Myr-gala’s amount was the lowest in the five cultivars. The total amount of flavonoid glycosides in Zhenghe Dabai tea flower was the lowest in the five cultivars. In three kinds of flavonoid glycosides, kaempferol glycosides’ content was the highest, and myricetin glycosides’ content was the lowest, and the majority of them were glycosylated with tri-glycosides in all the tested cultivars.

Key words: tea flower flavonoid glycosides ultra-high performance liquid chromatography-mass spectrometry tea plant cultivar

收稿日期: 2019-03-11 出版日期: 2020-01-20

CLC:

S 571.1

基金资助: 浙江省重点研发项目“茶树花果的功能因子和相关产品研发及产业化示范”(2018C02012)

通讯作者: 屠幼英 E-mail: jackson23sun@163.com;youytu@zju.edu.cn

作者简介: 李金（https://orcid.org/0000-0001-5601-9489），E-mail：jackson23sun@163.com

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引用本文:

李金,陈莲芙,金恩惠,李博,屠幼英. 不同茶树品种的茶树花黄酮苷研究[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(6): 707-714.

Jin LI,Lianfu CHEN,Eunhye KIM,Bo LI,Youying TU. Research on flavonoid glycosides of tea flower in different tea plant cultivars. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(6): 707-714.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2019.03.111 或 http://www.zjujournals.com/agr/CN/Y2019/V45/I6/707

表1 黄酮醇标准品的色谱和质谱信息

图1 茶树花中黄酮苷的UHPLC色谱图（λ=360 nm）

表2 茶树花中黄酮苷类物质的质谱信息

图2 各峰化合物的质谱图

表3 不同品种的茶树花黄酮苷含量

表4 不同品种茶树花中不同苷元的黄酮苷含量

表5 不同品种茶树花中不同糖基的黄酮苷含量

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