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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2019, Vol. 45 Issue (6): 685-691    DOI: 10.3785/j.issn.1008-9209.2018.12.201
园艺学     
低镁与丛枝菌根对枳砧‘纽荷尔’脐橙和‘椪柑’矿质营养吸收与分布的影响
杨艳(),胡成银,陈强,张晓平,肖家欣()
安徽师范大学生命科学学院，重要生物资源保护与利用研究安徽省省级重点实验室，安徽 芜湖 241000
Effects of magnesium-poor and arbuscular mycorrhizae on mineral nutrient uptake and distribution of ‘Newhall’ navel orange and ‘Ponkan’ tangerine grafted on rootstock trifoliate orange
Yan YANG(),Chengyin HU,Qiang CHEN,Xiaoping ZHANG,Jiaxin XIAO()
Provincial Key Laboratory for the Conservation and Utilization of Important Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui, China
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摘要：

采用盆栽实验研究低镁处理和丛枝菌根（arbuscular mycorrhiza, AM）真菌地表球囊霉（Glomus versiforme）接种处理对枳（Poncirus trifoliata）砧‘纽荷尔’脐橙[Citrus sinensis (L.) Osbeck cv. Newhall]和‘椪柑’（Citrus reticulata Blanco cv. Ponkan）不同部位硼、磷、钾和钙含量的影响。结果表明：低镁处理提高了‘纽荷尔’下部叶硼、磷及上部叶钾、钙含量，却降低了‘椪柑’下部叶硼、上部叶钾及上下部叶磷、钙含量。在镁适量条件下，AM真菌接种处理显著提高了‘纽荷尔’上部叶硼含量与分配比例、‘椪柑’各部位（上部叶除外）硼含量与分配比例，同时还显著提高了‘纽荷尔’接穗茎和砧木茎磷、钙及下部叶钾、钙含量及‘椪柑’各部位钾、根部磷与钙、上下部叶钙含量。在低镁条件下，AM真菌接种处理显著提高了2个品种各部位硼（‘纽荷尔’上部叶除外）、磷（‘纽荷尔’下部叶除外）、钾（‘椪柑’下部叶除外）和钙(‘椪柑’根部除外)含量，尤以‘纽荷尔’明显。说明在低镁条件下地表球囊霉接种处理可促进枳砧‘纽荷尔’脐橙苗对硼、磷、钾和钙的吸收与转运。研究结果可为柑橘通过平衡施肥来克服营养缺乏黄化症提供科学依据。
关键词： 丛枝菌根;  低镁;  砧穗互作;  硼;  ‘纽荷尔’脐橙;  ‘椪柑’    
Abstract:

The effects of magnesium-poor (Mg-poor) and arbuscular mycorrhizal (AM) fungus Glomus versiforme treatments on boron (B), phosphorus (P), potassium (K) and calcium (Ca) concentrations of different plant parts were investigated in potted ‘Newhall’ navel orange ［Citrus sinensis (L.) Osbeck cv. Newhall］ and ‘Ponkan’ tangerine (Citrus reticulata Blancocv. Ponkan) grafted on the rootstock trifoliate orange. The results showed that Mg-poor increased B and P concentrations in the basal leaves, and K and Ca concentrations in the upper leaves of ‘Newhall’, while decreased the concentrations of B in the basal leaves, K in the upper leaves, and P and Ca in the upper and basal leaves of ‘Ponkan’. Under the Mg-rich condition, AM fungus inoculation significantly increased B concentration and percentage in the upper leaves of ‘Newhall’, and B concentration and percentage in different plant parts (except for the upper leaf) of ‘Ponkan’. In addition, AM fungus inoculation significantly improved the concentrations of P and Ca in the scion and rootstock stems, and K and Ca in the basal leaves of ‘Newhall’, as well as K in different plant parts, P and Ca in the roots, and Ca in the upper and basal leaves of ‘Ponkan’. Under the Mg-poor condition, AM fungus inoculation significantly enhanced the concentrations of B (except for the upper leaf of ‘Newhall’), P (except for the basal leaf of ‘Newhall’), K (except for the basal leaf of ‘Ponkan’), and Ca (except for the root of ‘Ponkan’) in different plant parts of the two cultivars, especially in the ‘Newhall’. These results indicate that G. versiforme-inoculation can improve the absorption and transportation of B, P, K and Ca in ‘Newhall’ navel orange grafted on trifoliate orange exposed to Mg-poor soil in a greenhouse. It provides a scientific basis for balanced fertilization in citrus cultivation to overcome the yellowing symptoms of nutrient deficiency.
Key words: arbuscular mycorrhizae    magnesium-poor    scion-rootstock interaction    boron    ‘Newhall’ navel orange [Citrus sinensis (L.) Osbeck cv. Newhall]    ‘Ponkan’ tangerine (Citrus reticulata Blanco cv. Ponkan)
收稿日期: 2018-12-20 出版日期: 2020-01-20
CLC:  S 666  
基金资助: 国家自然科学基金(31372014);安徽省高等学校省级自然科学研究项目(KJ2016SD24)
通讯作者: 肖家欣     E-mail: 316949201@qq.com;xjx0930@163.com
作者简介: 杨艳（https://orcid.org/0000-0002-4079-7259），E-mail：316949201@qq.com
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杨艳
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引用本文:

杨艳,胡成银,陈强,张晓平,肖家欣. 低镁与丛枝菌根对枳砧‘纽荷尔’脐橙和‘椪柑’矿质营养吸收与分布的影响[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(6): 685-691.

Yan YANG,Chengyin HU,Qiang CHEN,Xiaoping ZHANG,Jiaxin XIAO. Effects of magnesium-poor and arbuscular mycorrhizae on mineral nutrient uptake and distribution of ‘Newhall’ navel orange and ‘Ponkan’ tangerine grafted on rootstock trifoliate orange. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(6): 685-691.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.12.201    或    http://www.zjujournals.com/agr/CN/Y2019/V45/I6/685

图1  低镁条件下AM真菌对‘纽荷尔’和‘椪柑’上部叶（A）、下部叶（B）、接穗茎（C）、砧木茎（D）及根部（E）硼含量的影响+AMF：接种丛枝菌根真菌地表球囊霉；－AMF：未接种丛枝菌根真菌地表球囊霉。P：‘椪柑’；N：‘纽荷尔’。MP：低镁处理；MR：镁适量处理。硼含量按干质量计。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
图2  低镁条件下AM真菌对‘纽荷尔’和‘椪柑’上部叶、下部叶、接穗茎、砧木茎及根部硼积累量占整株积累量的影响不同处理符号表示的含义详见图1注。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
图3  低镁条件下AM真菌对‘纽荷尔’和‘椪柑’上部叶（A）、下部叶（B）、接穗茎（C）、砧木茎（D）及根部（E）磷含量的影响不同处理符号表示的含义详见图1注。磷含量按干质量计。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
图4  低镁条件下AM真菌对‘纽荷尔’和‘椪柑’上部叶（A）、下部叶（B）、接穗茎（C）、砧木茎（D）及根部（E）钾含量的影响不同处理符号表示的含义详见图1注。钾含量按干质量计。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
图5  低镁条件下AM真菌对‘纽荷尔’和‘椪柑’上部叶（A）、下部叶（B）、接穗茎（C）、砧木茎（D）及根部（E）钙含量的影响不同处理符号表示的含义详见图1注。钙含量按干质量计。短栅上不同小写字母表示在P＜0.05水平差异有统计学意义。
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