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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2019, Vol. 45 Issue (4): 484-489    DOI: 10.3785/j.issn.1008-9209.2018.10.191
动物科学与动物医学     
齐卡兔瘦蛋白基因在不同组织中的表达水平与肉质指标的关联性
罗刚(),王杰,陈婷婷,刘林海,赖松家()
四川农业大学动物遗传育种研究所, 成都 611130
Correlation between leptin gene expression level in different tissues and meat quality in Zika rabbit
Gang LUO(),Jie WANG,Tingting CHEN,Linhai LIU,Songjia LAI()
Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China
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摘要：

选取齐卡兔（N系）为实验材料，利用荧光定量聚合酶链式反应（quantitative real-time fluorescence polymerase chain reaction, qRT-PCR）技术测定齐卡兔在35、70、84和91日龄4个阶段腿肌、肾周脂肪和皮下脂肪中瘦蛋白基因（leptin）的表达水平，并检测腿肌的pH值、熟肉率、滴水损失、肌内脂肪等相关肉品质指标，从而分析齐卡兔在这3种组织中leptin基因表达水平与所测肉品质指标的关联性。结果表明：母兔肾周脂肪和腿肌中leptin基因的表达量高于公兔，而在皮下脂肪中公兔的leptin基因表达量高于母兔。84日龄母兔皮下脂肪中leptin基因的表达量与肌内脂肪含量、熟肉率和屠宰后24 h的pH值的相关系数分别为0.95(P＜0.01)、0.83(P＜0.05)和0.67(P＜0.05)；公兔屠宰后24 h的pH值与腿肌中leptin基因表达量的相关系数为－0.85(P＜0.01）。根据分析，认为leptin基因在齐卡兔各组织中的表达量与肉质指标具有显著的相关性，可以作为研究家兔肉质的一个候选基因，从而为采用分子遗传标记技术和开展肉兔的遗传改良提供参考。
关键词： 瘦蛋白基因;  齐卡兔;  关联性分析;  肉质    
Abstract:

Zika rabbit (N strain) was selected as the experimental material, and the expression levels of leptin gene (leptin) in leg muscle, perirenal fat and subcutaneous fat at four different days of age (35, 70, 84, 91) of Zika rabbits were determined by quantitative real-time fluorescence polymerase chain reaction (qRT-PCR). The pH value of leg muscle, cooked meat rate, drip loss and intramuscular fat were also measured. The correlation between leptin gene expression level and meat quality index in above the three tissues of Zika rabbits was analyzed. The results showed that leptin gene expression in perirenal fat and leg muscle of female rabbits was higher than that of male rabbits, and the leptin gene expression in subcutaneous fat of male rabbits was higher than that of female rabbits. The correlation coefficients between the expression level of leptin gene in subcutaneous fat and intramuscular fat content, cooked meat rate, pH value of 24 h after slaughter in the 84 day-old female rabbits were 0.95 (P＜0.01), 0.83 (P＜0.05) and 0.67 (P＜0.05), respectively; and the correlation coefficient between pH value of 24 h after slaughter and the expression level of leptin gene in the leg muscle in the 84 day-old male rabbits was －0.85 (P＜0.01). According to the analysis result, the expression level of leptin gene in the tissues of Zika rabbits is significantly correlated with meat quality, and it can be used as a candidate gene for studying meat quality of rabbits, and provide a reference for using molecular genetic marker technology and genetic improvement of meat rabbits.
Key words: leptin gene    Zika rabbit    correlation analysis    meat quality
收稿日期: 2018-10-19 出版日期: 2019-09-17
CLC:  Q 756  
基金资助: 国家现代农业产业技术体系建设专项(CARS-44-A-2);四川省“十三五”畜禽育种攻关项目(2016NYZ0046)
通讯作者: 赖松家     E-mail: 2503917313@qq.com;LSJ5791@263.net
作者简介: 罗刚（https://orcid.org/0000-0003-0963-247X），E-mail：2503917313@qq.com
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罗刚
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刘林海
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引用本文:

罗刚,王杰,陈婷婷,刘林海,赖松家. 齐卡兔瘦蛋白基因在不同组织中的表达水平与肉质指标的关联性[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(4): 484-489.

Gang LUO,Jie WANG,Tingting CHEN,Linhai LIU,Songjia LAI. Correlation between leptin gene expression level in different tissues and meat quality in Zika rabbit. Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(4): 484-489.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.10.191    或    http://www.zjujournals.com/agr/CN/Y2019/V45/I4/484

基因名称Gene name 引物序列Primer sequence
leptin F: 5 ′ -CTGTGCCCATGCGGAAAGTC-3 ′
R: 5 ′ -AGTCCAAACCGACGACCCTC-3 ′
β-actin F: 5 ′ -GGAGATCGTGCGGGACAT-3 ′
R: 5 ′ -GTTGAAGGTGGTCTCGTGGAT-3 ′
表1  引物序列信息
图1   leptin 基因在齐卡兔各组织中的表达量

日龄

Days of age

 熟肉率Cooked meat rate w（肌内脂肪）Intramuscular fat content 滴水损失Drip loss
母兔 Female 公兔 Male 母兔 Female 公兔 Male 母兔 Female 公兔 Male
35 51.45±3.55dC 50.02±3.58cC 1.56±0.28dC 1.40±0.21dC 13.76±1.88aA 12.40±1.81aA
70 53.52±3.12cC 51.51±3.14cC 2.11±0.51cB 2.01±0.62cB 7.56±1.51bB 7.31±1.62bB
84 58.01±5.45bB 57.21±3.55bB 2.24±0.65bA 2.17±0.56bA 5.45±1.35cC 5.55±1.26cC
91 63.35±5.99aA 62.13±5.87aA 2.35±0.69aA 2.23±0.57aA 4.96±1.09cC 4.88±1.01cC
表2  不同日龄齐卡兔的熟肉率、肌内脂肪含量和滴水损失

日龄

Days of age

 母兔 Female 公兔 Male

屠宰后45 min

45 min after slaughter

屠宰后24 h

24 h after slaughter

屠宰后45 min

45 min after slaughter

屠宰后24 h

24 h after slaughter
35 6.26±0.29cB 5.85±0.25cB 6.28±0.35dB 5.90±0.28cB
70 6.45±0.31bA 6.01±0.28bA 6.49±0.35cB 6.11±0.22aA
84 6.56±0.35aA 6.11±0.36aA 6.58±0.39bA 6.15±0.26aA
91 6.62±0.49aA 5.95±0.41bA 6.66±0.55aA 5.99±0.31bA
表3  不同日龄齐卡兔屠宰后肌肉的pH值

leptin基因

leptin gene

肌内脂肪含量

Intramuscular fat content

熟肉率

Cooked meat rate

滴水损失

Drip loss

pH值1）

pH value1)

在皮下脂肪中表达量

Expression level in subcutaneous fat

 母兔 Female 0.95** 0.83* －0.43 0.67*
公兔 Male －0.12 －0.90** －0.28 －0.52

在肾周脂肪中表达量

Expression level in perirenal fat

 母兔 Female －0.086 0.12 －0.28 0.33
公兔 Male －0.57 －0.89** －0.33 0.17

在腿肌中表达量

Expression level in leg muscle

 母兔 Female 0.16 －0.38 0.47 0.12
公兔 Male －0.54 －0.28 －0.04 －0.85**
表4  84日龄齐卡兔leptin基因表达水平与肉质性状的相关性分析
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