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浙江大学学报（农业与生命科学版）
浙江大学学报（农业与生命科学版）  2019, Vol. 45 Issue (4): 401-406    DOI: 10.3785/j.issn.1008-9209.2018.06.210
园艺学     
菜用豌豆核心种质构建的关键策略
徐盛春1(),郑华章2,冯志娟1,刘娜1,张古文1,胡齐赞1,龚亚明1()
1. 浙江省农业科学院蔬菜研究所，杭州 310021
2. 余姚市农业技术推广服务总站，浙江 余姚 315400
Key construction strategies for core germplasm collection in vegetable pea (Pisum sativum)
Shengchun XU1(),Huazhang ZHENG2,Zhijuan FENG1,Na LIU1,Guwen ZHANG1,Qizan HU1,Yaming GONG1()
1. Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. Yuyao Agricultural Technical Extension and Service Center, Yuyao 315400, Zhejiang, China
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摘要：

利用已搜集的180份菜用豌豆材料进行核心种质构建策略研究。分别对所有种质材料进行单株荚数、每荚粒数、荚长、荚宽、荚厚、百荚鲜质量、百粒鲜质量及产量等性状进行调查，结果表明，搜集的材料具有丰富的遗传多样性。利用上述数据，采用最小距离逐步取样（minimum distance stepwise sampling, LDSS）法，分别选择4种遗传距离、8种取样比例进行核心种质构建策略研究，并采用极差符合率（coincidence rate of range, CR）和变异系数变化率（variable rate of coefficient of variation, VR）2个参数对构建策略进行评价；同时，利用主成分分析法和聚类分析法对构建的核心种质代表性进行鉴定。结果表明，采用LDSS法构建菜用豌豆核心种质的最佳遗传距离为欧式距离，最佳取样比例为25%。该构建策略将为菜用豌豆核心种质构建与高效利用奠定基础。
关键词： 菜用豌豆;  核心种质构建;  最小距离逐步取样法;  欧式距离    
Abstract:

Core germplasm construction is one of the important means to utilize and manage plant germplasm resources. In present study, 180 germplasms of vegetable pea (Pisum sativum) were collected to study the construction strategy of core germplasm. The agronomic and yield characters were investigated, including the number of pod per plant, number of seed per pod, pod length, pod width, pod thickness, fresh mass of 100-pod, fresh mass of 100-seed, and yield. The results revealed an abundant genetic diversity in these vegetable pea germplasms. Using these data, the construction strategies of core germplasm were studied by using the minimum distance stepwise sampling (LDSS) method with four genetic distances and eight sampling ratios. Then, the construction strategies were evaluated by using the coincidence rate of range (CR) and variable rate of coefficient of variation (VR). The principal component analysis and cluster analysis were conducted to validate the representativeness of core germplasms. The results showed that Euclidean distance (ED) combining 25% sampling ratio was the best construction strategy in core germplasm construction of vegetable pea based on LDSS method. This study will lay a foundation for the core germplasm construction and efficient utilization of vegetable pea.
Key words: Pisum sativum    core germplasm construction    least distance stepwise sampling method    Euclidean distance
收稿日期: 2018-06-21 出版日期: 2019-09-17
CLC:  S 324  
基金资助: 国家自然科学基金(31572138);国家重点研发计划“七大作物育种”重点专项(2018YFD01009001);浙江省农业（蔬菜）新品种选育重大科技专项(2016C02051)
通讯作者: 龚亚明     E-mail: shengchun2001@163.com;gongym07@126.com
作者简介: 徐盛春（https://orcid.org/0000-0001-8135-0990），E-mail：shengchun2001@163.com
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引用本文:

徐盛春,郑华章,冯志娟,刘娜,张古文,胡齐赞,龚亚明. 菜用豌豆核心种质构建的关键策略[J]. 浙江大学学报（农业与生命科学版）, 2019, 45(4): 401-406.

Shengchun XU,Huazhang ZHENG,Zhijuan FENG,Na LIU,Guwen ZHANG,Qizan HU,Yaming GONG. Key construction strategies for core germplasm collection in vegetable pea (Pisum sativum). Journal of Zhejiang University (Agriculture and Life Sciences), 2019, 45(4): 401-406.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2018.06.210    或    http://www.zjujournals.com/agr/CN/Y2019/V45/I4/401

性状

Characters

变异范围

Range of variation

平均值

Average value
单株荚数（荚/株） Number of pod per plant/(pod/plant) 13.9～26.5 21.1
每荚粒数（粒/荚） Number of seed per pod/(seed/pod) 5.6～7.5 6.6
荚长 Pod length/cm 7.1～8.3 7.8
荚宽 Pod width/cm 1.2～1.8 1.5
荚厚 Pod thickness/cm 0.9～1.3 1.1
百荚鲜质量 Fresh mass of 100-pod/g 409.8～806.6 591.8
百粒鲜质量 Fresh mass of 100-seed/g 35.6～59.4 46.2
产量 Yield/(kg/667 m2) 508.2～812.4 655.0
表1  180份菜用豌豆种质田间性状

参数

Parameter

遗传距离

Genetic distance

 取样比例 Sampling ratio/%
5 10 15 20 25 30 35 40
CR ED 76.11 87.84 93.76 95.36 96.35 96.35 96.35 96.35
MD 68.93 83.30 88.82 91.88 92.80 92.80 92.80 92.80
CBD 79.65 85.66 88.91 91.10 93.00 95.55 97.41 97.41
Cos-D 57.87 66.23 72.70 77.94 80.05 81.14 82.88 83.49
Cor-D 56.08 70.77 74.68 77.46 80.74 82.59 82.59 87.84
VR ED 124.64 123.95 122.35 122.55 119.03 116.22 114.08 113.36
MD 117.60 124.60 119.55 117.27 116.08 115.18 113.38 111.61
CBD 133.04 123.61 118.68 116.54 115.16 113.72 112.96 111.96
Cos-D 101.56 100.18 100.89 106.42 106.92 105.14 103.48 103.08
Cor-D 103.36 104.80 100.71 102.30 103.07 104.05 102.38 102.66
表2  菜用豌豆核心种质评价参数比较
图1  菜用豌豆群体的主成分分析
图2  菜用豌豆原始群体和核心种质聚类图
1 宗绪晓,关建平,王述民,等 .国外栽培豌豆遗传多样性分析及核心种质构建.作物学报,2008,34(9):1518-1528.
ZONG X X , GUAN J P , WANG S M , et al . Genetic diversity and core collection of alien Pisum sativum L. germplasm. Acta Agronomica Sinica, 2008,34(9):1518-1528. (in Chinese with English abstract)
2 李自超,张洪亮,曹永生,等 .中国地方稻种资源初级核心种质取样策略研究.作物学报,2003,29(1):20-24.
LI Z C , ZHANG H L , CAO Y S , et al . Studies on the sampling strategy for primary core collection of Chinese ingenious rice. Acta Agronomica Sinica, 2003,29(1):20-24. (in Chinese with English abstract)
3 魏兴华,颜启传,应存山,等 .建立浙江地方籼型稻种资源的核心样品的研究.中国水稻科学,1999,13(2):81-85.
WEI X H , YAN Q C , YING C S , et al . A core collection of Zhejiang traditional indica rice germplasm. Chinese Journal of Rice Science, 1999,13(2):81-85. (in Chinese with English abstract)
4 RAO E S , KADIRVEL P , SYMONDS R C , et al . Using SSR markers to map genetic diversity and population structure of Solanum pimpinellifolium for development of a core collection. Plant Genetic Resources, 2012,10(1):38-48.
5 ZHANG J , WANG Y , ZHANG X Z , et al . Sampling strategy to develop a primary core collection of apple cultivars based on fruit traits. African Journal of Biotechnology, 2010,9(2):123-127.
6 UPADHYAYA H D , SARMA N , RAVISHANKAR C R , et al . Developing a mini-core collection in finger millet using multilocation data. Crop Science, 2010,50(5):1924-1931.
7 KIM K W , CHUNG H K , CHO G T, et al . PowerCore: a program applying the advanced M strategy with a heuristic search for establishing core sets. Bioinformatics, 2007,23(16):2155-2162.
8 GOUESNARD B , BATAILLON T M , DECOUX G , et al . MSTRAT: an algorithm for building germ plasm core collections by maximizing allelic or phenotypic richness. Journal of Heredity, 2001,92(1):93-94.
9 THACHUK C , CROSSA J , FRANCO J , et al . Core Hunter: an algorithm for sampling genetic resources based on multiple genetic measures. BMC Bioinformatics, 2009,10(1):243-245.
10 MEI Y J , ZHOU J P , XU H M , et al . Development of sea island cotton (Gossypium barbadense L.) core collection using genotypic values. Australian Journal of Crop Science, 2012,6(4):673-680.
11 BROWN A H D . Core collection: a practical approach to genetic resources management. Genome, 1989,31(2):818-824.
12 WANG J C , HU J , ZHANG C F , et al . Assessment on evaluating parameters of rice core collections constructed by genotypic values and molecular marker information. Rice Science, 2007,14(2):101-110.
13 WANG J C , HU J , XU H M , et al . A strategy on constructing core collections by least distance stepwise sampling. Theoretical and Applied Genetics, 2007,115(1):1-8.
14 HU J , ZHU J , XU H M . Methods of constructing core collections by stepwise clustering with three sampling strategies based on the genotypic values of crops. Theoretical and Applied Genetics, 2000,101(1/2):264-268.
15 DIEZ C M , IMPERATO A , RALLO L , et al . Worldwide core collection of olive cultivars based on simple sequence repeat and morphological markers. Crop Science, 2012,52(1):211-221.
16 QUERO-GARCIA J , NOYER J L , PERRIER X , et al . A germplasm stratification of taro (Colocasia esculenta) based on agro-morphological descriptors, validation by AFLP markers. Euphytica, 2004,137(3):387-395.
17 高志红,章镇,韩振海,等 .中国果梅核心种质的构建与检测.中国农业科学,2005,38(2):363-368.
GAO Z H , ZHANG Z , HAN Z H , et al . Development and evaluation of core collection of Japanese apricot germplasms in China. Scientia Agricultura Sinica, 2005,38(2):363-368. (in Chinese with English abstract)
18 明军,张启翔,兰彦平 .梅花品种资源核心种质构建.北京林业大学学报,2005,27(2):65-69.
MING J , ZHANG Q X , LAN Y P . Core collection of Prunus mume Sieb. et Zucc. Journal of Beijing Forestry University, 2005,27(2):65-69. (in Chinese with English abstract)
19 邱丽娟,曹永生,常汝镇,等 .中国大豆(Glycine max)核心种质构建Ⅰ.取样方法研究.中国农业科学,2003,36(12):1442-1449.
QIU L J , CAO Y S , CHANG R Z , et al . Establishment of Chinese soybean (G . max) core collection Ⅰ . Sampling strategy. Scientia Agricultura Sinica, 2003,36(12):1442-1449. (in Chinese with English abstract)
20 ZEWDIE Y , TONG N K , BOSLAND P . Establishing a core collection of capsicum using a cluster analysis with enlightened selection of accessions. Genetic Resources and Crop Evolution, 2004,51(2):147-151.
21 徐盛春,王建成,胡齐赞,等 .菜用大豆核心种质构建策略研究.科技通报,2012,28(8):36-41.
XU S C , WANG J C , HU Q Z , et al . Investigation on constructing strategies of core collection in green soybean. Bulletin of Science and Technology, 2012,28(8):36-41. (in Chinese with English abstract)
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