Parameters of wheat crop growth model WOFOST (world food studies) were discriminated, and the sensitivity of crop and soil parameters in WOFOST model was analyzed using OAT (one-at-a-time) analysis method. Meanwhile, the simulation results of wheat growth were explained. Results showed that the relative sensitivities of seven parameters of WOFOST model, including TSUM1, FRTB, CVO, CVS, DVSI, SPAN, SLATB, were all greater than 0.5, which presented strong sensitivity and had greater influence on the model simulation results. The parameters which had greater influence on the WOFOST model based on different levels of yield and production were almost the same, and the relative sensitivity had no significant difference. Simulation results of leaf area index (LAI) showed that the days of wheat from sowing to flowering were 137, and the days of wheat from flowering to maturity were 66, which was in accordance with the actual days. This study provides a basis for the application and parameter adjustment of WOFOST model.