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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2017, Vol. 43 Issue (3): 365-370    DOI: 10.3785/j.issn.1008-9209.2016.05.111
动物科学与动物医学     
福建黄兔肌纤维性状及Myh6基因表达水平研究
穆琳，王文洲，赵博昊，胡帅帅，赵斌，陈阳，吴信生*
扬州大学动物科学与技术学院，江苏省动物繁育与分子设计重点实验室，江苏 扬州 225009
Analysis of muscle fiber traits and Myh6 gene expression level in Fujian yellow rabbit
MU Lin, WANG Wenzhou, ZHAO Bohao, HU Shuaishuai, ZHAO Bin, CHEN Yang, WU Xinsheng*
(Jiangsu Key Laboratory of Animal Genetics & Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China)
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摘要： 选取我国地方品种福建黄兔为研究对象，利用冰冻切片技术检测不同发育阶段福建黄兔背肌和腿肌的肌纤维性状，通过荧光定量聚合酶链式反应技术分析在肌肉发育过程中起重要作用的Myh6基因在福建黄兔不同发育阶段表达水平的差异，并与肌纤维性状进行相关性分析。结果显示：2、4、6、8、10和12周龄福建黄兔的白肌纤维密度均显著大于红肌纤维和中间型肌纤维，白肌纤维的面积显著高于红肌纤维，长径和短径的大小排列为白肌纤维＞中间型肌纤维＞红肌纤维，但差异无统计学意义；在6周龄前，背肌和腿肌中各类肌纤维长径、短径和面积增大的趋势明显，而6周龄后增大趋势逐渐变缓；Myh6基因在公母兔背部和腿部的表达均呈先上升后下降的趋势，其中母兔在4周龄表达量最高，公兔在6周龄表达量最高；Myh6基因的表达量与肌纤维面积、长径和短径的变化一致。表明Myh6基因与肌纤维类型和肌纤维的生长发育密切相关。
Abstract: With growing demands for the diversity and quality of meat, rabbit meat is more and more popular with its special flavor. The traits of muscle fibers have a direct effect on the quality of meat, and the number, size, density, and type of muscle fibers are closely related to the meat flavor. However, recent studies on genes related to meat quality of rabbit are quite few, so the formation mechanism of the edibility characteristic of the muscle fibers is still unclear. Myh6, a member of the myosin heavy chain family, plays an important role in the development of muscles. What’s more, Myh6 is a marker gene in cardiac development which promotes the expansion of ventricular myocardial cell.
In order to clarify the relationship of Myh6 and muscle fiber traits, correlation analysis was used to study the expression level of Myh6 in rabbit muscle fibers of longissimus dorsi muscle and leg muscle with different ages, which could also provide theoretical basis for some improvement in rabbit meat. In this study, Fujian yellow rabbits were used as experimental animals, and their myofiber traits of longissimus dorsi muscle and leg muscles at different development stages were evaluated by a frozen section technique. Meanwhile, expression levels of Myh6 gene of Fujian yellow rabbit at different development stages were analyzed by quantitative reverse transcription-polymerase chain reaction（RT-PCR）.
The results showed that the density of white muscle fibers was significantly higher than that of red muscle fibers and intermediate muscle fibers at 2, 4, 6, 8, 10 and 12 weeks old; and the area of white muscle fibers was significantly greater than that of red muscle fibers. The descending order for the long and short diameters of muscle fibers of legs and backs was white muscle fibers, intermediate muscle fibers and red muscle fibers. The inverse relationship between the density and area of muscle fibers was that the density of muscle fibers decreased with the increase of muscle fiber area. The density of muscle fibers had an obviously decreasing trend from 2 to 6 weeks old. In the whole, with the age increasing, the density decreased but not significant. There was no significant difference between long and short diameters of muscle fibers, and both long and short diameters of the different muscle fibers increased gradually with the age increasing, although the trend was very obvious before 6 weeks old, but insignificant after 6 weeks old.
The results also showed that the expression level of Myh6 first increased and then decreased in longissimus dorsi muscles and leg muscles of bucks and does, which showed the highest expression level at 4 weeks old in does and 6 weeks old in bucks. Furthermore, there is no difference of the change tendency between the expression level of Myh6 gene and the variation of area, long and short diameters of muscle fibers. Above all, it can be concluded that the development of muscle fibers is related to Myh6 gene.
收稿日期: 2016-05-11 出版日期: 2016-11-23
CLC:  S 829.1  
基金资助: 国家现代农业产业技术体系建设专项资金（CARS-44-1）；江苏省高校优势学科建设工程项目（PAPD 2014-134）。
通讯作者: 吴信生(http://orcid.org/ 0000-0001-9871-0251)     E-mail: xswu@yzu.edu.cn
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引用本文:

穆琳，王文洲，赵博昊，胡帅帅，赵斌，陈阳，吴信生. 福建黄兔肌纤维性状及Myh6基因表达水平研究[J]. 浙江大学学报(农业与生命科学版), 2017, 43(3): 365-370.

MU Lin, WANG Wenzhou, ZHAO Bohao, HU Shuaishuai, ZHAO Bin, CHEN Yang, WU Xinsheng. Analysis of muscle fiber traits and Myh6 gene expression level in Fujian yellow rabbit. Journal of Zhejiang University (Agriculture and Life Sciences), 2017, 43(3): 365-370.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2016.05.111    或    http://www.zjujournals.com/agr/CN/Y2017/V43/I3/365

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