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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2015, Vol. 41 Issue (6): 641-649    DOI: 10.3785/j.issn.1008-9209.2015.05.121
生物科学与技术     
雨生红球藻提取物对糖尿病小鼠血糖及血脂水平的干预和调节作用
黄河儒1,2， 王姗姗1， 倪晓锋1， 陈日萍2， 张佳莉2， 陈翼翔3， 沈生荣1*
1.浙江大学生物系统工程与食品科学学院，杭州 310058;2.浙江省医学科学院，杭州 310013;3.浙江传媒学院电子信息学院，杭州 310018
Intervention and regulation effect of Haematococcus pluvialis extract on blood glucose and lipid of diabetic mice.
Huang Heru1,2, Wang Shanshan1, Ni Xiaofeng1, Chen Riping2, Zhang Jiali2, Chen Yixiang3, Shen Shengrong1*
(1. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; 2. Zhejiang Academy of Medical Sciences, Hangzhou 310013, China; 3. School of Electronics and Information, Zhejiang University of Media and Communications, Hangzhou 310018, China)
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摘要： 通过造小鼠糖尿病模型并给予不同剂量的雨生红球藻提取物,研究其对糖尿病造模小鼠糖耐量和胰岛素耐量的影响、血脂的调节情况及改善糖尿病病情的可能的作用机制。通过四氧嘧啶腹腔注射的方式建立糖尿病小鼠模型，灌胃给予不同剂量的雨生红球藻提取物，给药期为28 d，每7 d记录1次小鼠空腹血糖及体质量；给药期末进行耐糖量和胰岛素耐量实验，并测定血清甘油三酯（triglyceride，TG）、血清总胆固醇（serum total cholesterol，TC）、高密度脂蛋白胆固醇（high density lipoprotein cholesterol，HDL-C）和低密度脂蛋白胆固醇（low density lipoprotein cholesterol，LDL-C）含量。结果表明：造模后小鼠毛色杂乱暗淡，出现明显多饮、多食、多尿的“三多”症状，小鼠体质量明显减轻。试验组给予不同剂量雨生红球藻提取物28 d后，高、中、低剂量组的体质量与模型对照组比较均有统计学上的显著差异（P<0.01，P<0.05，P<0.05），高、中剂量组的血糖与模型组比较均有统计学上的显著差异（P<0.05，P<0.05），且以高剂量组最为明显。在糖耐量实验中，各组小鼠的血糖值均在糖负荷30 min时达到峰值，30 min后血糖值随着时间的增加呈下降趋势；高剂量组和中剂量组血糖值在120 min时明显低于模型对照组（P<0.01）。在胰岛素耐量实验中，腹腔注射0.8 U/kg胰岛素15 min后，高剂量组血糖值较基础值降低20.9％，而低剂量组仅降低0.6%；3个剂量组在90 min时均降低至最低值，血糖分别降低53.6%、23.9%和17.9%，模型组血糖降低5.3%；120 min时血糖已上升，但雨生红球藻提取物高剂量组血糖值仍显著低于模型对照组（P<0.01）。雨生红球藻提取物高剂量组血清TC、TG及LDL-C含量明显低于模型对照组，差异具有统计学意义（P<0.05，P<0.01，P<0.05）。总之，雨生红球藻提取物可显著降低造模糖尿病小鼠的血糖水平，缓解多饮、多食、多尿及体质量减轻症状，显著改善病鼠机体的糖耐量及胰岛素耐量，提升其对葡萄糖的利用能力，有效调节病鼠的血脂紊乱，改善血液环境，且对正常小鼠的血糖、血脂无显著影响。在实验所用的3个剂量中，以高剂量（8 g/kg）效果最佳。
Abstract: Diabetes was generally recognized as a critical issue in global public health, and has become the third noninfectious disease after angiocardiopathy and cancer. So far, the combination of applying insulin and controlling diet, as well as changing the way of living were the major therapeutic method for treating diabetes. Recently, the adjuvant therapy of natural extracts has been the research highlight in this field. To observe the intervention effect of Haematococcus pluvialis extract on blood glucose tolerance, insulin tolerance, lipid adjustment and the possible functional mechanism of improving diabetic patients’ condition, the diabetic mouse models were induced by alloxan through intraperitoneal injection, and given by gavage with different doses of H. pluvialis extract for 28 days. The contents of fasting blood glucose and their body masses were detected at 7-day intervals. The capability of blood glucose tolerance and insulin tolerance was measured during later period of administration. Meanwhile, the contents of triacylglycerol (TG), serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were detected. After modeling, the mice had dark and messy hair, and the typical diabetic symptoms including polydipsia, polyphagia and polyuria along with significant mass loss. Compared with the model group, by giving different doses of H. pluvialis extract in 28 d for experimental groups, the high, middle and low dose groups had statistically significant differences between the body masses and blood glucose contents, respectively (P<0.01, P<0.05, P<0.05). The difference between high dose group and model group was most evident. During the experiment of blood glucose tolerance, the blood glucose level of each group reached the peak in 30 min of glucose loading. The glucose levels appeared downtrend after 30 min, of which the high dose group and middle dose group were much lower than the model group in 120 min (P<0.01). In the insulin experiment, after injecting 0.8 U/kg insulin for 15 min, the blood glucose level of high dose group reduced by 20.9% compared with the basic level, while the low dose group only reduced by 0.6%. Three dose groups had the lowest blood glucose levels which reduced by 53.6%, 23.9% and 17.9% respectively in 90 min, while the model group reduced by 5.3%. The blood glucose increased in 120 min; however, the level in the high dose group of H. pluvialis extract was still much lower than that of model group (P<0.01). The TC, TG and LDL-C contents of the high dose group of H. pluvialis extract were much lower than the model group, which were statistically significant differences (P<0.05, P<0.01, P<0.05). Haematococcus pluvialis extract has remarkable effect of decreasing blood glucose level, relieving the symptoms of polydipsia, polyphagia, polyuria and mass loss, improving the glucose tolerance and insulin tolerance, increasing the utilization of glucose and regulating blood lipid level on diabetic mice. However, it has no effect on the blood glucose and lipids of normal mice. Among the three dose groups, the high dose group (8 g/kg) has the best effect.
出版日期: 2015-11-20
CLC:  R 282.71  
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黄河儒
王姗姗
倪晓锋
陈日萍
张佳莉
陈翼翔
沈生荣

引用本文:

黄河儒， 王姗姗，倪晓锋，陈日萍，张佳莉，陈翼翔，沈生荣. 雨生红球藻提取物对糖尿病小鼠血糖及血脂水平的干预和调节作用[J]. 浙江大学学报(农业与生命科学版), 2015, 41(6): 641-649.

Huang Heru, Wang Shanshan, Ni Xiaofeng, Chen Riping, Zhang Jiali, Chen Yixiang, Shen Shengrong. Intervention and regulation effect of Haematococcus pluvialis extract on blood glucose and lipid of diabetic mice.. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(6): 641-649.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2015.05.121    或    http://www.zjujournals.com/agr/CN/Y2015/V41/I6/641

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