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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2015, Vol. 41 Issue (2): 228-236    DOI: 10.3785/j.issn.1008-9209.2014.08.112
资源与环境科学     
壳聚糖生物炭微球对甲基红的吸附及微球菌剂的吸附增益效应
杜敬霆1， 孙朋飞2， 赵宇华2， 张昕1*
1.浙江农林大学林业与生物技术学院，浙江 临安 311300；2.浙江大学生命科学学院微生物研究所，杭州 310058
Biosorption of chitosan-biochar microsphere for methyl red and biosorption enhancing effect of microsphere-microbe complex.
Du Jingting1, Sun Pengfei2, Zhao Yuhua2, Zhang Xin1*
(1. College of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University, Lin’an 311300, Zhejiang, China; 2. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou 310058, China)
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摘要： 以典型偶氮染料甲基红为吸附对象，将壳聚糖和生物炭混合制成微球制剂，对该制剂吸附甲基红的潜力、机制及添加微生物后微球菌剂的吸附增益效应进行研究。结果表明，所制得的壳聚糖生物炭微球具有良好的吸附特性，在染料初始质量浓度为500 mg/L、pH 3.0和20 ℃条件下，微球对甲基红的最大吸附量为460 mg/g。Freundlich等温方程和准二级动力学方程模型与该吸附过程拟合良好。利用Elovich方程与颗粒内扩散方程对微球的吸附过程进行研究，结果证实该吸附过程为自发过程，内部扩散为该过程的限速步骤。通过热力学方程计算得到吸附过程的热力学参数ΔG0、ΔH0和ΔS0分别为－24.35 kJ/mol(30 ℃)、－21.36 kJ/mol和8.45 J/(mol?K)。表明此吸附过程为放热过程，较高温度可降低微球吸附的自发性并抑制微球对甲基红的吸附作用。利用神经网络对影响吸附过程的4个因素（pH值，温度，初始浓度，吸附时间）进行建模分析，发现温度对吸附过程的影响最大，相对重要性为32.76%，时间次之(为25.56%)，其余依次为pH(23.36%)和初始浓度(18.32%)。包埋微生物的微球菌剂与无微生物的纯壳聚糖生物炭微球相比，脱色率提高了29%；与微生物单独脱色相比，脱色率提高了76%，表明生物吸附材料与微生物混合制备成菌剂的吸附增益效果明显.
Abstract: Dyes are widely and intensively used in leather, textile, paper, printing, cosmetic, plastic, food and pharmaceutical industries. Most of them pose serious threats to the environment because of their carcinogenic potential and cytotoxicity. Hence, effluents containing dye molecule must be treated to minimize the threat to the ecosystem. Adsorption has been found to be one of the prominent techniques for dye wastewater treatment considering cost and efficiency. While the main disadvantage of physical adsorption is its simple transfer of the dye molecules to another phase rather than complete destruction. Therefore, it is meaningful to find an efficient adsorbent and explore the synergistic effect of the prepared adsorbent by combining adsorption material with dye-biodegrading microorganisms. Methyl red was selected as model azo dyes in this study to investigate the biosorption behavior of newly produced chitosan-biochar microsphere, and meanwhile the enhancing effect of microsphere-microbe complex on removal of the dye was evaluted. To study the biosorption mechanism and capacity of chitosan-biochar microsphere for methyl red, the Langmuir and Freundlich adsorption isotherms were used to examine the system of methyl red and microsphere. The pesudo-first-order and pesudo-second-order models were used to describe the biosorption kinetics. Elovich equation and inter-diffusion model were applied to interpret the process of adsorption. In this work, artificial neural network (ANN) model was selected to analyze the parameters that affecting adsorption. The input layer had four neurons such as pH, initial dye concentration, temperature and adsorption time. The output layer had one neuron as adsorption capacity. Finally, degrading strain Bacillus sp. UN2 was immobilized by the microspheres to remove the methyl red in solutions, and the enhancing removal effect of microsphere-microbe complex for the dye was explored. The results showed that the chitosan-biochar microsphere was an efficient adsorbent, and the maximum biosorption capacity of 460 mg/g for methy red was observed under the initial dye concentration of 500 mg/L, pH 3.0 and 20 °C conditions. The data of biosorption were fitted with Freundlich isotherm and the pseudo-second-order kinetic model successfully. The data based on Elovich equation and inter-diffusion mode proved the dominant status of adsorption against desorption. The values of thermo-dynamic parameters ΔG0, ΔH0 and ΔS0 were －24.35 kJ/mol (30 ℃), －21.36 kJ/mol and 8.45 J/(mol?K) respectively. Therefore, the adsorption was a spontaneous, exothermic process, with inter-diffusion as the limiting step for biosorption of methyl red onto biosorbent. Artificial neural network model analysis indicated that the temperature appeared to be the most influential parameter for biosorption process with a relative importance of 32.76%, followed by adsorption time (25.56%), pH (23.36%) and initial dye concentration (18.32%). Immobilization with microsphere could improve the tolerance level of degrading strain Bacillus sp. UN2 in methyl red solution with high concentration, and the removal rate for methyl red was increased by 29% and 76% respectively by chitosan-biochar-microbe complex compared with chitosan-biochar microsphere and free strains alone. In conclusion, the newly produced chitosan-biochar microsphere is an efficient adsorption material for methyl red. The maximum biosorption capacity is better than ever reported. The adsorption is a spontaneous, exothermic process, with the inter-diffusion as the limiting step for biosorption of methyl red onto biosorbent. Temperature is the most influential parameter for biosorption process. Significantly enhancing adsorption effect is achieved when the chitosan-biochar microsphere immobilized the degrading strain Bacillus sp. UN2, which offers an environment-friendly and cost-competitive method to treat the dye pollution.
出版日期: 2015-03-20
CLC:  X 172  
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杜敬霆
孙朋飞
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张昕

引用本文:

杜敬霆，孙朋飞，赵宇华，张昕. 壳聚糖生物炭微球对甲基红的吸附及微球菌剂的吸附增益效应[J]. 浙江大学学报(农业与生命科学版), 2015, 41(2): 228-236.

Du Jingting, Sun Pengfei, Zhao Yuhua, Zhang Xin. Biosorption of chitosan-biochar microsphere for methyl red and biosorption enhancing effect of microsphere-microbe complex.. Journal of Zhejiang University (Agriculture and Life Sciences), 2015, 41(2): 228-236.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2014.08.112    或    http://www.zjujournals.com/agr/CN/Y2015/V41/I2/228

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