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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (6): 624-630    DOI: 10.3785/j.issn.1008-9209.2011.06.006
生物科学与技术     
雨生红球藻异养转化产虾青素的条件研究
张睿钦, 管斌, 孔青, 游泳, 吴继芹, 魏群
中国海洋大学食品科学与工程学院,山东青岛２６６００３
Study on astaxanthin accumulation by heterotrophic transformation in Haematococcus pluvialis
ZHANG Rui‐qin , GUAN Bin , KONG Qing , YOU Yong , WU Ji‐qin , WEI Qun
Institute o f Food Science and Engineering , Ocean University o f China , Qingdao , Shandong 266003 ,China
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摘要： 对比自养转化与异养转化的虾青素产量,发现向培养基中添加乙酸钠进行异养转化可使羽生红球藻的虾青素产量由０.７２３ ６ mg.L － １ 提高到１.２１６ ８ mg.L － １ ;为进一步提高虾青素产量,在单因素实验基础上,选择乙酸钠浓度和硝酸钾浓度为自变量,虾青素产量为响应值,利用中心组合设计实验和响应面分析法,对转化培养基进行优化.结果表明:异养转化培养基中的乙酸钠和硝酸钾浓度对虾青素产量影响较大,在乙酸钠１.４６０ ５ g.L － １ ,硝酸钾０.００８ ４ g.L － １ ,磷酸二氢钾０.０２ g.L － １ 时,虾青素产量最
高,达１０.９０８ mg.L － １ ,与对照相比提高了１０.１６ 倍,与自养转化相比提高了１４.０７ 倍.
Abstract: The effects of heterotrophic transformation and autotrophic transformation on astaxanthin production in H aematococcus p luv ialis were studied and compared . When sodium acetate was added in MCM for heterotrophic transformation ,astaxanthin accumulation was increased from ０ .７２３ ６ mg.L － １ to １.２１６ ８ mgL － １ .To enhance the astaxanthin accumulation , single factor experiment was founded up.Center composite design experiments and response surface methodology were employed to optimize the media of heterotrophic transformation.The results showed a distinctive influence of sodium acetate and potassium nitrate concentrations on astaxanthin accumulation.The optimal fermentation medium consisted of １.４６０ ５ g.L － １ sodium acetate , ０.００８ ４ g.L － １ potassium nitrate , and ０.０２ g.L － １ potassium dihydrogen phosphate.Astaxanthin content reached １０.９０８ mg .L － １ , which was raised １０.１６‐fold against the control group and １４.０７‐fold against the group of autotrophic transformation.
出版日期: 2011-11-25
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引用本文:

张睿钦, 管斌, 孔青, 游泳, 吴继芹, 魏群. 雨生红球藻异养转化产虾青素的条件研究[J]. 浙江大学学报(农业与生命科学版), 2011, 37(6): 624-630.

ZHANG Rui‐qin,GUAN Bin,KONG Qing,YOU Yong,WU Ji‐qin,WEI Qun. Study on astaxanthin accumulation by heterotrophic transformation in Haematococcus pluvialis. , 2011, 37(6): 624-630.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.06.006    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I6/624

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