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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (6): 596-602    DOI: 10.3785/j.issn.1008-9209.2011.06.002
生物科学与技术     
花椰菜菌核病抗性鉴定方法比较及抗病种质资源筛选
孙溶溶１ , 彭真１ , 程琳１ , 邵泰良２ , 卢钢１
１ .浙江大学农业与生物技术学院蔬菜研究所,浙江杭州３１００５８;２ .浙江神良种业有限公司,浙江温州３２５０１１
Comparison of identification methods for resistance to Sclerotinia sclerotiorum and screening of resistant materials in cauliflower
SUN Rong‐rong１, PENG Zhen１, CHENG Lin１, SHAO Tai‐liang２, LU Gang１
1 . Institute of Vegetable Science , College of Agriculture & Biotechnology , Zhejiang University , Hangzhou 310058 ,China ; 2 . Zhejiang Sky Good Seed Co . L td . , Wenzhou , Zhejiang 325011 , China
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摘要： 采用已报道的３种十字花科作物菌核病抗病性鉴定方法,在同一条件下,对５个已知抗性的花椰菜品种进行鉴定比较.结果表明:结球期琼脂块叶腋接种法、营养生长期琼脂块活体叶片法与大田接种鉴定的相关系数分别达到０ .８８５ ９和０ .９２６ ６ ,可有效反映抗、感品种的差异,可用于花椰菜菌核病抗性的快速鉴定;但营养生长期琼脂块离体叶片法用于抗性鉴定的效果不好;根据抗性鉴定方法的比较结果,选用结球期琼脂块叶腋接种法,以 农乐４５ 天为对照,对２１份花椰菜材料进行抗性鉴定,筛选获得了 神良金花 和 长胜65天 ２份高抗材料.
Abstract: Three identification methods for the resistance to Sclerotinia sclerotiorum previously‐reported in Brassicaceae crops were compared using ５cauliflower varieties under the same condition to develop the effective screening technique in cauliflower . The results showed that the correlation between inoculating mycelium agar disk to leaf axil at seedling stage ,or inoculating mycelium agar disk to live leaf at heading stage and field inoculation were highly significant ( r ＝０.８８５９and ０.９２６６, respectively) , so these two methods could well identify resistance and susceptibility of cauliflower varieties . The method of inoculating mycelium agar disk to in v itro seedling leaf was not suitable for resistance identification of cauliflower . To screen resistant sources of cauliflower against S . sclerotiorum , ２１ breeding materials were tested with the method of inoculating mycelium agar disk to leaf axil at heading stage , using cauliflower variety  nongle ４５ days as control . The results showed that there were two high resistant materials ,  shenliang golden flower and  changsheng ６５days .
出版日期: 2011-11-25
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孙溶溶
程琳
邵泰良
卢钢
彭真

引用本文:

孙溶溶, 彭真, 程琳, 邵泰良, 卢钢. 花椰菜菌核病抗性鉴定方法比较及抗病种质资源筛选[J]. 浙江大学学报(农业与生命科学版), 2011, 37(6): 596-602.

SUN Rong‐rong, PENG Zhen, CHENG Lin, SHAO Tai‐liang,LU Gang. Comparison of identification methods for resistance to Sclerotinia sclerotiorum and screening of resistant materials in cauliflower. , 2011, 37(6): 596-602.

链接本文:

http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.06.002    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I6/596

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