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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (4): 393-398    DOI: 10.3785/j.issn.1008-9209.2011.04.006
生物科学与技术     
紫菜薹花青素合成酶基因BcANS的克隆、表达与序列分析
蒋 明1,陈孝赏2,李金枝1
１ .台州学院生命科学学院,浙江 临海 ３１７０００ ; ２ .台州市农业科学研究院, 浙江 临海 ３１７０００
Cloning, expression and sequence analysis of anthocyanidin synthase gene BcANS in Brassica campestris var. purpurea
JIANG Ming１ , CHEN Xiao-shang２ , LI Jin-zhi１
1 . College of Life Science , Taizhou University ,Linhai , Zhejiang 317000 , China ; 2 . Taizhou Academy of Agricultural Sciences , Linhai , Zhejiang 317000 , China
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摘要： 根据前期在芥蓝中克隆的BaANS 基因序列设计PCR 引物,从紫菜薹( Brassica campestris var . purpurea)子叶中克隆到基因组DNA 和cDNA 序列,基因定名为BcANS ;序列已提交NCBI 数据库,登录号为GQ１２０５６２ ;BcA N S 的基因组DNA 全长为１ ６３７ bp ,具１ 个５６０ bp 的内含子,编码区全长为１ ０７７ bp ,编码３５８ 个氨基酸.RT-PCR 检测结果表明:BcANS 在光照条件下表达,在暗培养植株的子叶和胚轴中未见表达;缺磷植株的子叶和胚轴在暗培养和光照培养下BcANS 基因均有表达,但光照条件下的表达量较大.序列比对结果表明:BcANS 与同科的甘蓝、芥菜和拟南芥的同源性最高,而与禾本科植物的同源性最低,在进化树上相距最远.对紫菜薹花青素合成酶基因的克隆和表达分析,为进一步开展基因功能和花青素生物合成机制研究奠定了基础.
Abstract: PCR primers were designed according to BaANS gene isolated from Brassica albograbra , and the gene , designated BcANS , was amplified from cotyledons DNA and cDNA of B . campestris var . purpurea , respectively . The gene sequence was submitted to NCBI with an accession number of GQ１２０５６２ . Genomic DNA was １ ６３７ bp in length with one ５６０ bp intron , and the complete coding sequence was １ ０７７ bp encoding ３５８ amino acids . RT-PCR results showed that BcA N S expressed in cotyledons and hypocotyls under light condition , and there was no expression under dark condition . BcA N S expressed under dark and light conditions in phosphorus-deficient medium . However , higher expression levels in cotyledons and hypocotyls were observed under light condition . Sequence alignment results revealed higher homology between BcANS and those of B . oleracea , B . j uncea and A rabidopsis thaliana . Low similarity was observed between BcANS and those of Gramineae plants , indicating their distant evolutionary relationships . Isolation , sequence analysis of anthocyanidin synthase gene in B .campestris var . purpurea laid the foundation for further study of gene function and anthocyanin biosynthesis mechanisms .
出版日期: 2011-07-20
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蒋 明
陈孝赏
李金枝

引用本文:

蒋 明,陈孝赏,李金枝. 紫菜薹花青素合成酶基因BcANS的克隆、表达与序列分析[J]. 浙江大学学报(农业与生命科学版), 2011, 37(4): 393-398.

JIANG Ming,CHEN Xiao-shang,LI Jin-zhi. Cloning, expression and sequence analysis of anthocyanidin synthase gene BcANS in Brassica campestris var. purpurea. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(4): 393-398.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.04.006    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I4/393

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