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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2011, Vol. 37 Issue (4): 387-392    DOI: 10.3785/j.issn.1008-9209.2011.04.005
生物科学与技术     
黄瓜白粉病抗性序列相关扩增多态性的分子标记研究
景 然1,陈新娟2,朱育强2,张 鹏2,张玉琼1,周胜军2
１ .安徽农业大学生命科学学院,安徽 合肥 ２３００３６ ; ２ .浙江省农业科学院蔬菜研究所, 浙江 杭州 ３１００２１
Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber
JING Ran１ , CHEN Xin-juan２ , ZHU Yu-qiang２ , ZHANG Peng２ , ZHANG Yu-qiong１ , ZHOU Shengjun２
1 . School of Life Science , Anhui Agricultural University , Hefei 230036 , China ;2 . Institute of Vegetables , Zhejiang Academy of Agricultural Sciences , Hangzhou 310021 , China
 全文: PDF(3532 KB)   HTML ( ) 
摘要： 以高抗和高感白粉病的黄瓜亲本及其杂交后代F２ 分离群体为材料,应用序列相关扩增多态性(sequence-related amplified polymorphism , SRAP) 技术开展与黄瓜白粉病抗性基因连锁的SRAP 分子标记研究.结果表明:从４３７ 对SRAP 引物组合中筛选出６２ 对能够在２ 个亲本抗、感池间扩增出稳定多态性条带的引物组合,进一步用这些引物分析F２ 群体,发现有１ 对引物组合Me１/Em９ 扩增出的SRAP标记(Me1/Em9----284 bp)能与黄瓜抗白粉病基因连锁,遗传距离为９ .８ cM .获得的分子标记对克隆抗病基因和利用分子标记辅助选择提高育种效率具有重要意义.
Abstract: With a F２ population between a resistant parent and susceptible parent , SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber . Sixty‐two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and susceptible parent were selected from ４３７ pairs of SRAP primer combinations . Further analyzing the F２ segregating populations using these primers , one SRAP marker of Me1 /Em9 — 284 bp linked to the powdery mildew resistance was found , with 9.8 cM genetic distance . The marker can be used for cloning disease‐resistant gene and advancing efficiency of disease-resistant breeding on molecular marker assisted selection .
出版日期: 2011-07-20
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陈新娟
张玉琼
景 然
朱育强
张 鹏
周胜军

引用本文:

景 然,陈新娟,朱育强,张 鹏,张玉琼,周胜军. 黄瓜白粉病抗性序列相关扩增多态性的分子标记研究[J]. 浙江大学学报(农业与生命科学版), 2011, 37(4): 387-392.

JING Ran,CHEN Xin-juan,ZHU Yu-qiang,ZHANG Peng,ZHANG Yu-qiong,ZHOU Sheng-jun. Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber. Journal of Zhejiang University: Agric. & Life Sci., 2011, 37(4): 387-392.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2011.04.005    或    http://www.zjujournals.com/agr/CN/Y2011/V37/I4/387

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