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浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2010, Vol. 36 Issue (6): 683-690    DOI: 10.3785/j.issn.1008-9209.2010.06.014
资源与环境科学     
不同品种荞麦耐铝性的FTIR鉴别
张亚楠,王赢,陈奇,黄庶识,李昆志
张亚楠,昆明理工大学生物工程技术研究中心,云南昆明650224; 王赢,昆明理工大学生物工程技术研究中心,云南昆明650224; 陈奇,昆明理工大学生物工程技术研究中心,云南昆明650224; 黄庶识,昆明理工大学生物工程技术研究中心,云南昆明650224;广西科学院生物物理实验室,广西南宁530007 李昆志,昆明理工大学生物工程技术研究中心,云南昆明650224;
Endurance determination of different buckwheat cultivars to aluminum by FTIR spectrum
ZHANG Ya-nan,WANG Ying,CHEN Qi,HUANG Shu-shi,LI Kun-zhi
ZHANG Ya-nan,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China; WANG Ying,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China; CHEN Qi,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China; HUANG Shu-shi,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China;Lab of Biophysics,Guangxi Academy of Sciences,Nanning530007,China LI Kun-zhi,Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China;
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摘要： 通过傅里叶变换红外光谱法(FTIR)结合常规的根伸长实验法对2个不同耐铝性的荞麦品种 (春红1号和春红4号)进行分析鉴定 。通过比较春红1号和春红4号在不同铝浓度处理下根、茎和叶的 红外光谱图特征峰3353、2923、1734、1643、1453、1367、1250和1055 cm-1发现 :春红1号的特征峰 值高于春红4号 ;在铝胁迫下,春红1号和春红4号的根、茎和叶的 FTIR特征峰值随着铝浓度的升高 而增加,当铝浓度增至50μm ol.L-1时,春红1号达到最大值 ;而春红4号在20μm ol.L-1时达到最大 值 ;此外,在铝胁迫下,春红4号根、茎和叶的 FTIR特征峰值的变化幅度比春红1号大,说明春红4号 物质代谢受到的影响较大,表现出对铝敏感 。根伸长实验结果表明 :当铝浓度为20μm ol.L-1时,促进春 红1号根的生长,根伸长量是对照的140%,达到最大值,而春红4号的根生长受到很大的抑制,伸长量 只有对照的80% 。以上研究结果表明,春红1号比春红4号的耐铝能力强,FTIR法可以用作鉴别不同 品种荞麦耐铝性的一种新的、快速、准确的检验方法 。
Abstract: The Al-tolerance of two different varieties (Chun-Hong1,Chun-Hong4) of buckwheat was analyzed and identified by Fourier transform infrared (FTIR) spectroscopy and conventional experimental method of root elongation . By comparison of root,stem and leaf characteristics of the infrared spectra peaks3353,2 923,1 734,1 643,1 453,1 367,1 250 and1055 cm-1 between the two varieties under the different Al concentrations,most of the characteristic peaks in Chun-Hong1 were higher than that in Chun-Hong4 .Characteristics of the FTIR peak for the root,stem and leaf in Chun-Hong1 and Chun-Hong4 were increased with increase of Al concentrations . FTIR peak of Chun-Hong 1 reached the maximum when the Al concentration increased to50μm ol.L-1,while that of Chun-Hong4 reached the maximum at the Al concentration of20μm ol.L-1 .Besides,the results showed that the FTIR spectra of Chun-Hong4 varied much greater in roots,stems and leaves than aluminum-resistant cultivar Chun-Hong1 under different Al concentrations,implying that Chun-Hong4 metabolism was evidently affected by Al . The results of root elongation experiment showed that the root growth of Chun-Hong 1 was promoted and the amount of root elongation reached the maximum of140% compared with the control,while that of Chun-Hong4 was greatly inhibited and its elongation was only80% of the control with the treatment of20μm ol.L-1 Al . These findings demonstrate that Al endurance of Chun-Hong1 is stronger than Chun-Hong4,and FTIR spectra can be developed as a new,rapid and accurate method for the endurance identification of different buckwheat cultivars to Al .
出版日期: 2010-11-09
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张亚楠
王赢
陈奇
黄庶识
李昆志

引用本文:

张亚楠,王赢,陈奇,黄庶识,李昆志. 不同品种荞麦耐铝性的FTIR鉴别[J]. 浙江大学学报(农业与生命科学版), 2010, 36(6): 683-690.

ZHANG Ya-nan,WANG Ying,CHEN Qi,HUANG Shu-shi,LI Kun-zhi. Endurance determination of different buckwheat cultivars to aluminum by FTIR spectrum. Journal of Zhejiang University: Agric. & Life Sci., 2010, 36(6): 683-690.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2010.06.014    或    http://www.zjujournals.com/agr/CN/Y2010/V36/I6/683

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