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浙江大学学报(农业与生命科学版)  2010, Vol. 36 Issue (6): 657-661    DOI: 10.3785/j.issn.1008-9209.2010.06.010
农业科学     
一种解磷菌剂的研制及其应用效果
叶明,陶涛,陈吴西
叶明,合肥工业大学生物与食品工程学院,安徽合肥230009 陶涛,合肥工业大学生物与食品工程学院,安徽合肥230009 陈吴西,合肥工业大学生物与食品工程学院,安徽合肥230009
Development and application of a phosphate-dissolving bacteria agent
YE Ming,TAO Tao,CHEN Wu-xi
YE Ming,Schoolof Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China TAO Tao,Schoolof Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009,China CHEN Wu-xi,Schoolof Biotechnology and Food Engineering,Hefei University of Technology,Hefei230009, China
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摘要: 利用 C53组合设计对5株具有较强溶磷能力的解磷细菌进行混合发酵,得到具有良好协同作 用的菌株组合(Bc、Bs、Ap1 ),通过正交试验确定其降解土壤中难溶性磷的最佳接种量为Bc2%、Bs6%、 Ap16% 。按此接种比例,在解磷菌剂发酵培养基中接种各菌株,并将其在温度为30℃、摇床转速为140 r.min-1条件下培养28 h,即制得解磷菌剂,并进行白菜盆栽试验,结果表明该菌剂对白菜根长、株高及 地上部鲜质量均具有良好的促进作用 。
Abstract: Five bacterial strains with strong phosphate-solubilizing ability were mixed fermentation by C53 composite design,and the co-fermentation systems consisted of the strains Bc,Bs,Ap1 were obtained . The optimum inoculation in degradation of insoluble phosphate in soil was developed through orthogonal design,and the best inoculation ratio was Bc 2%,Bs 6%,Ap1 6% . It was a phosphate-dissolving bacteria agent that the three strains were inoculated in the phosphate-dissolving bacteria fermentation medium according to above-mentioned inoculation ratio and cultured at 30℃and shaking speed 140 r. min-1 for 28 h . Pot experiment results illustrated that the phosphate-solubilizing bacteria had a good advance on the plant height,root length and shoot fresh mass of Chinese cabbage .
出版日期: 2010-11-09
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引用本文:

叶明,陶涛,陈吴西. 一种解磷菌剂的研制及其应用效果[J]. 浙江大学学报(农业与生命科学版), 2010, 36(6): 657-661.

YE Ming,TAO Tao,CHEN Wu-xi. Development and application of a phosphate-dissolving bacteria agent. Journal of Zhejiang University: Agric. & Life Sci., 2010, 36(6): 657-661.

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http://www.zjujournals.com/agr/CN/10.3785/j.issn.1008-9209.2010.06.010        http://www.zjujournals.com/agr/CN/Y2010/V36/I6/657

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