Please wait a minute...
浙江大学学报（农业与生命科学版）
浙江大学学报(农业与生命科学版)  2010, Vol. 36 Issue (6): 602-608    DOI: 10.3785/j.issn.1008-9209.2010.06.002
生物科学与技术     
利用Wx 基因功能性标记选育中等直链淀粉含量优质水稻保持系
周屹峰,赵霏,任三娟,包劲松,沈圣泉
周屹峰,浙江大学农业与生物技术学院原子核农业科学研究所,浙江杭州310029; 赵霏,浙江大学农业与生物技术学院原子核农业科学研究所,浙江杭州310029; 任三娟,浙江大学农业与生物技术学院原子核农业科学研究所,浙江杭州310029; 包劲松,浙江大学农业与生物技术学院原子核农业科学研究所,浙江杭州310029; 沈圣泉,浙江大学农业与生物技术学院原子核农业科学研究所,浙江杭州310029.
Developing indicamaintainer lines with intermediate amylose content by Wx functional microsatellite marker in rice
ZHOU Yi-feng,ZHAO Fei,REN San-juan,BAO Jin-song,SHEN Sheng-quan
ZHOU Yi-feng,Institute of Nuclear- Agricultural Sciences,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China ZHAO Fei,Institute of Nuclear- Agricultural Sciences,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China REN San-juan,Institute of Nuclear- Agricultural Sciences,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China BAO Jin-song,Institute of Nuclear- Agricultural Sciences,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China SHEN Sheng-quan,Institute of Nuclear- Agricultural Sciences,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China
 全文: PDF(3370 KB)   HTML ( ) 
摘要： 利用 Wx 基因功能性标记 484/485"对国内13份籼型保持系进行多态性鉴定和筛选。结果显示:检测出的 Wx 基因型与本品种直链淀粉含量(AC)有良好的对应关系 ;利用 484/485"标记对Ⅱ-32B/宜香1B"配组后代(F1～F3代)进行 PCR鉴定的结果表明,484/485"标记与稻米直链淀粉含量是紧密连锁、共分离的,经 F2代分子辅助选择,F3代株系的 484/485"Ⅰ型系比例高达93.5%,在 F3代 获得较多 484/485"Ⅰ型株系的基础上,结合株型、粒型、垩白度、透明度、糊化温度等多项指标的田间和室内选择,于 F6代获得了114份 484/485"为Ⅰ型,且株型良好、粒型细长、低垩白、高透明度、低糊 化温度的优质株系,其中,有92.1 %入选系的直链淀粉含量在15%～20%之间 ;进一步测交鉴定还表 明,这批入选优质株系有74.5 %为保持系。说明利用 484/485"标记辅助选择、高效改良或选育中等直链淀粉含量的优质保持系(或不育系)是可行的 。
Abstract: Genotypes of the 13 indica maintainer lines were detected by the Wx gene functional microsatellite marker 484/485" . The results showed that there was significant association between the Wx genotypes and amylose contents of rice . Analysis of progenies in the generations F1 to F3 derived fromⅡ-32B/Yixiang1B" confirmed that 484/485" microsatellite marker was tightly linked to and co-segregated with rice amylose content . After carrying out marker-assisted selection in F2 generation,individual plants with the homozygous Wx-Ⅰgenotype selected from F3 generation reached as high as 93.5 % . The phenotypes such as plant type,grain shape,chalkiness,transparency,gelatinization temperature,etc. were characterized either in the field or in the lab . Finally,a total of 114 target breeding lines with Wx-Ⅰg enotype,good plant types,slender grain type,low degree of chalkiness,high degree of transparency,intermediate gelatinization temperature and amylose contents were successfully obtained from the F6 generation . Ninety-two point one percent of the breeding lines had the amylose contents between15% and 20% . Results of testcross showed that 74.5 % of the breeding lines were maintainer lines . It is suggested that developing indica maintainer lines with intermediate amylose contents by Wx microsatellite marker is feasible and effective .
出版日期: 2010-11-09
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　

引用本文:

周屹峰,赵霏,任三娟,包劲松,沈圣泉. 利用Wx 基因功能性标记选育中等直链淀粉含量优质水稻保持系[J]. 浙江大学学报(农业与生命科学版), 2010, 36(6): 602-608.

链接本文:

http://www.zjujournals.com/agr/CN/Y2010/V36/I6/602

No related articles found!