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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2013, Vol. 14 Issue (2): 97-105    DOI: 10.1631/jzus.B1200159
Articles     
Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide
Bing Feng, Lai-ji Ma, Jin-jing Yao, Yun Fang, Yan-ai Mei, Shao-min Wei
School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China; R&D Center, Shanghai Jahwa United Co., Ltd., Shanghai 201702, China; Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai 200433, China
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Abstract  Oat contains different components that possess antioxidant properties; no study to date has addressed the antioxidant effect of the extract of oat bran on the cellular level. Therefore, the present study focuses on the investigation of the protective effect of oat bran extract by enzymatic hydrolysates on human dermal fibroblast injury induced by hydrogen peroxide (H2O2). Kjeldahl determination, phenol-sulfuric acid method, and high-performance liquid chromatography (HPLC) analysis indicated that the enzymatic products of oat bran contain a protein amount of 71.93%, of which 97.43% are peptides with a molecular range from 438.56 to 1 301.01 Da. Assays for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity indicate that oat peptide-rich extract has a direct and concentration-dependent antioxidant activity. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay and the TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay for apoptosis showed that administration of H2O2 in human dermal fibroblasts caused cell damage and apoptosis. Pre-incubation of human dermal fibroblasts with the Oatp for 24 h markedly inhibited human dermal fibroblast injury induced by H2O2, but application oat peptides with H2O2 at same time did not. Pre-treatment of human dermal fibroblasts with Oatp significantly reversed the H2O2-induced decrease of superoxide dismutase (SOD) and the inhibition of malondialdehyde (MDA). The results demonstrate that oat peptides possess antioxidant activity and are effective against H2O2-induced human dermal fibroblast injury by the enhanced activity of SOD and decrease in MDA level. Our results suggest that oat bran will have the potential to be further explored as an antioxidant functional food in the prevention of aging-related skin injury.

Key words： Oat bran      Extraction      Antioxidant      Human dermal fibroblasts      Cell injury     
Received: 04 June 2012      Published: 31 January 2013
CLC:  Q28  
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Bing Feng
Jin-jing Yao
Lai-ji Ma
Yun Fang
Yan-ai Mei
Shao-min Wei
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Bing Feng, Lai-ji Ma, Jin-jing Yao, Yun Fang, Yan-ai Mei, Shao-min Wei. Protective effect of oat bran extracts on human dermal fibroblast injury induced by hydrogen peroxide. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(2): 97-105.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1200159     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2013/V14/I2/97

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