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| Demonstration of a new biosensing concept for immunodiagnostic applications based on change in surface conductance of antibodies after biomolecular interactions |
| VASHIST Sandeep Kumar, KAUR Inderpreet, BAJPAI Ram Prakash, BHARADWAJ Lalit Mohan, TEWARI Rupinder, RAITERI Roberto |
| Department of Biophysical and Electronic Engineering, University of Genoa, via Opera Pia 11A, Genoa-16145, Italy; Biomolecular Electronics and Nanotechnology Division, Central Scientific Instruments Organization, Sector 30, Chandigarh-160030, India; Department of Biotechnology, Panjab University, Sector 14, Chandigarh-160014, India |
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Abstract We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass substrate decreases upon the formation of complex with their specific antigens. This change in conductivity has been observed for both monoclonal and polyclonal antibodies. The conductance of monoclonal mouse IgG immobilized on polylysine-coated glass substrate changed from 1.02×10−8 Ω−1 to 1.41×10−11 Ω−1 at 10 V when complex is formed due to the specific biomolecular interactions with rabbit anti-mouse IgG F(ab′)2. Similar behavior was observed when the same set up was tested in two clinical assays: (1) anti-Leishmania antigen polyclonal antibodies taken from Kala Azar positive patient serum interacting with Leishmania promastigote antigen, and (2) anti-p21 polyclonal antibodies interacting with p21 antigen. The proposed concept can represent a new immunodiagnostic technique and may have wide ranging applications in biosensors and nanobiotechnology too.
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Received: 03 July 2006
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