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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2016, Vol. 17 Issue (4): 262-270    DOI: 10.1631/jzus.B1500120
Articles     
Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger
Wei Hu, Ji-hong Chen, Shu-yang Wang, Jing Liu, Yuan Song, Qing-feng Wu, Wen-jian Li
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key laboratory of Microbial Resources Exploition and Application, Lanzhou 730000, China
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Abstract  The objective of this work was to produce citric acid from corn starch using a newly isolated mutant of Aspergillus , and to analyze the relationship between changes in the physiological properties of A. induced by carbon ion irradiation and citric acid accumulation. Our results showed that the physiological characteristics of conidia in A. niger were closely related to citric acid accumulation and that lower growth rate and viability of conidia may be beneficial to citric acid accumulation. Using corn starch as a raw material, a high-yielding citric acid mutant, named HW2, was obtained. In a 10-L bioreactor, HW2 can accumulate 118.9 g/L citric acid with a residual total sugar concentration of only 14.4 g/L. This represented an 18% increase in citric acid accumulation and a 12.5% decrease in sugar utilization compared with the original strain.

Key words： Carbon ion irradiation      Physiological properties      Mutation      Citric acid accumulation     
Received: 16 May 2015      Published: 05 April 2016
CLC:  Q819  
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Wei Hu
Ji-hong Chen
Shu-yang Wang
Jing Liu
Yuan Song
Qing-feng Wu
Wen-jian Li
Cite this article:

Wei Hu, Ji-hong Chen, Shu-yang Wang, Jing Liu, Yuan Song, Qing-feng Wu, Wen-jian Li. Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(4): 262-270.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1500120     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2016/V17/I4/262

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