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| A mutation of Aspergillus niger for hyper-production of citric acid from corn meal hydrolysate in a bioreactor |
| Wei Hu, Jing Liu, Ji-hong Chen, Shu-yang Wang, Dong Lu, Qing-hua Wu, Wen-jian Li |
| Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Beijing 100049, China; Key laboratory of Microbial Resources Exploition and Application, Lanzhou 730000, China |
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Abstract The properties of the screened mutants for hyper-production of citric acid induced by carbon (12C6+) ion beams and X-ray irradiation were investigated in our current study. Among these mutants, mutant H4002 screened from 12C6+ ion irradiation had a higher yield of citric acid production than the parental strain in a 250-ml shaking flash. These expanded submerged experiments in a bioreactor were also carried out for mutant H4002. The results showed that (177.7–196.0) g/L citric acid was accumulated by H4002 through exploiting corn meal hydrolysate (containing initial 200.0–235.7 g/L sugar) with the productivity of (2.96–3.27) g/(L∙h). This was especially true when the initial sugar concentration was 210 g/L, and the best economical citric acid production reached (187.5±0.7) g/L with a productivity of 3.13 g/(L∙h). It was observed that mutant H4002 can utilize low-cost corn meal as a feedstock to efficiently produce citric acid. These results imply that the H4002 strain has the industrial production potentiality for citric acid and offers strong competition for the citric acid industry.
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Received: 08 May 2014
Published: 04 November 2014
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