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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (12): 952-957    DOI: 10.1631/jzus.B1000073
Biotechnology     
Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation
Hong-yun Liu, Wei-dong Zeng, Ai-ling Cao, Cai-qiao Zhang
Key Laboratory of Animal Epidemic Etiology and Immunological Prevention of the Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China, Xiaoshan Entry-Exit Inspection and Quarantine Bureau, Hangzhou 311208, China
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Abstract  The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) pathways. Ovarian cells were treated with FSH alone or in the presence of forskolin (FRSK), PKA inhibitor (H89), PKC activator (PMA) or inhibitor (H7). The germ cell number was counted from micropictures. The immunocytochemistry of proliferating cell nuclear antigen (PCNA) was applied to identify the proliferating cells. The germ cell labeling index (LI) was determined for cell proliferation. The FSH treatment increased the germ cell number, and this stimulating effect was enhanced by FRSK or PMA, but inhibited by H89 or H7 in a dose-dependent manner. Moreover, the PCNA-LI showed parallel changes with germ cell numbers. This study suggests that FSH may stimulate proliferation of cultured chicken ovarian germ cells by activation of both the PKA and PKC signaling pathways.

Key words： Follicle-stimulating hormone      Protein kinase A      Protein kinase C      Germ cell      Proliferation     
Received: 05 March 2010      Published: 09 December 2010
CLC:  Q25  
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Ai-ling Cao
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Hong-yun Liu, Wei-dong Zeng, Ai-ling Cao, Cai-qiao Zhang. Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(12): 952-957.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1000073     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I12/952

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