| Biomedicine |
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| Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering |
| Chun-mao Han, Li-ping Zhang, Jin-zhang Sun, Hai-fei Shi, Jie Zhou, Chang-you Gao |
| Department of Burn, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China |
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Abstract To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications.
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Received: 07 December 2009
Published: 04 July 2010
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