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Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2016, Vol. 17 Issue (11): 864-873    DOI: 10.1631/jzus.B1600151
Articles     
In vitro remineralization of hybrid layers using biomimetic analogs
Hui-ping Lin, Jun Lin, Juan Li, Jing-hong Xu, Christian Mehl
Department of Stomatology, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; HarderMehl Dental Clinic, Volkartstra?e 5, 80634 Munich, Germany
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Abstract  Resin-dentin bond degradation is a major cause of restoration failures. The major aim of the current study was to evaluate the impact of a remineralization medium on collagen matrices of hybrid layers of three different adhesive resins using nanotechnology methods. Coronal dentin surfaces were prepared from freshly extracted premolars and bonded to composite resin using three adhesive resins (FluoroBond II, Xeno-III-Bond, and iBond). From each tooth, two central slabs were selected for the study. The slabs used as controls were immersed in a simulated body fluid (SBF). The experimental slabs were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs (biomineralization medium (BRM)). Eight slabs per group were retrieved after 1, 2, 3, and 4 months, respectively and immersed in Rhodamine B for 24 h. Confocal laser scanning microscopy was used to evaluate the permeability of hybrid layers to Rhodamine B. Data were analyzed by analysis of variance (ANOVA) and Tukey’s honest significant difference (HSD) tests. After four months, all BRM specimens exhibited a significantly smaller fluorescent area than SBF specimens, indicating a remineralization of the hybrid layer (P≤0.05). A clinically applicable biomimetic remineralization delivery system could potentially slow down bond degradation.

Key wordsRemineralization      Dentin      Adhesive resin      Biomimetic analog      Altered collagen      Confocal laser scanning microscopy (CLSM)      Fluorescence     
Received: 05 April 2016      Published: 03 November 2016
CLC:  R783.1  
Cite this article:

Hui-ping Lin, Jun Lin, Juan Li, Jing-hong Xu, Christian Mehl. In vitro remineralization of hybrid layers using biomimetic analogs. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2016, 17(11): 864-873.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1600151     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2016/V17/I11/864

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