| Biomedicine |
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| Determination of theophylline concentration in serum by chemiluminescent immunoassay |
| ZHOU Mei-xia, GUAN Cha-ying, CHEN Guang, XIE Xin-you, WU Sheng-hai |
| Department of Laboratory, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China; Department of Laboratory, Hangzhou First Hospital, Hangzhou 310006, China |
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Abstract Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay. Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 μmol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.
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Received: 25 July 2005
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