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| Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease |
| An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang, Si-yuan Li, Qiang Fu, Clare E. Hugnes, Bruce Caterson |
| Institute of Endemic Diseases, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China, Key Laboratory of Microelement and Endemic Disease of Ministry of Health, Xi'an Jiaotong University, Xi'an 710061, China, 4?Laboratory of Connective Tissue Biology, School of Biosciences, Cardiff University, Cardiff CF10 3US, UK |
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Abstract Objective: To investigate the effects of mycotoxin moniliformin (MON) on the metabolism of aggrecan and type II collagen in human chondrocytes in vitro and the relationship between MON and Kashin-Beck disease (KBD). Methods: Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without MON toxin. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of aggrecan and type II collagen in the cartilage was determined using immunocytochemical staining. Results: MON toxin inhibited chondrocyte viability in dose-dependent and time-dependent manners. MON reduced aggrecan and type II collagen syntheses in the tissue-engineered cartilage. MON also increased the expression of matrix metalloproteinase-1 (MMP-1), MMP-13, BC4 epitopes, and CD44 in cartilages. However, the expression of 3B3(−) epitopes in cartilages was inhibited by MON. Selenium partially alleviated the damage of aggrecan induced by MON toxin. Conclusion: MON toxin promoted the catabolism of aggrecan and type II collagen in human chondrocytes.
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Received: 12 March 2009
Published: 10 March 2010
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Cite this article:
An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang, Si-yuan Li, Qiang Fu, Clare E. Hugnes, Bruce Caterson. Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(3): 200-208.
URL:
http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0900074 OR http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I3/200
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