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ZJUS-B
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2010, Vol. 11 Issue (7): 506-511    DOI: 10.1631/jzus.B0900365
Biotechnology     
In vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis
Hossein Motamedi, Esmaeil Darabpour, Mahnaz Gholipour, Seyyed Mansour Seyyed Nejad
Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran
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Abstract  Brucellosis, a zoonosis caused by four species of brucella, has a high morbidity. Brucella melitensis is the main causative agent of brucellosis in both human and small ruminants. As an alternative to conventional antibiotics, medicinal plants are valuable resources for new agents against antibiotic-resistant strains. The aim of this study was to investigate the usage of native plants for brucellosis treatment. For this purpose, the anti-brucella activities of ethanolic and methanolic extracts of Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, Plantago ovata, Cordia myxa, and Crocus sativus were assessed. The activity against a resistant Br. melitensis strain was determined by disc diffusion method at various concentrations from 50–400 mg/ml. Antibiotic discs were also used as a control. Among the evaluated herbs, six plant (Salvia sclarea, Oliveria decumbens, Ferulago angulata, Vitex pseudo-negundo, Teucrium polium, and Crocus sativus) showed anti-brucella activity. Oliveria decumbens was chosen as the most effective plant for further studies. A tested isolate exhibited resistance to tetracycline, nafcillin, oxacillin, methicillin, and colistin. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values for Oliveria decumbens against resistant Br. melitensis were the same (5 mg/ml), and for gentamicin they were both 2 mg/ml. Time-kill kinetics for a methanolic extract of Oliveria decumbens was 7 h whereas for an ethanolic extract it was 28 h. Also, Oliveria decumbens extracts showed a synergistic effect in combination with doxycycline and tetracycline. In general, the similar values of MIC and MBC for Oliveria decumbens suggest that these extracts could act as bactericidal agents against Br. melitensis. In addition to Oliveria decumbens, Crocus sativus and Salvia sclarea also had good anti-brucella activity and these should be considered for further study.

Key words： Brucellosis      Antibiotic resistance      Brucella melitensis      Medicinal plant      Oliveria decumbens     
Received: 13 November 2009      Published: 04 July 2010
CLC:  Q939.92  
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Seyyed Mansour Seyyed Nejad
Hossein Motamedi
Esmaeil Darabpour
Mahnaz Gholipour
Cite this article:

Hossein Motamedi, Esmaeil Darabpour, Mahnaz Gholipour, Seyyed Mansour Seyyed Nejad. In vitro assay for the anti-brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2010, 11(7): 506-511.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B0900365     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2010/V11/I7/506

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