| Plant & Animal Sciences and Biotechnology |
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| Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field |
| ZHANG Xin, ZHANG Bing-xin, ZHANG Zhen, SHEN Wei-feng, YANG Ching-hong, YU Jing-quan, ZHAO Yu-hua |
| Department of Plant Protection, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; Academy of Agriculture Science, Hangzhou 310021, China; Department of Biological Sciences, University of Wisconsin-Milwaukee, WI 53211, USA; Department of Horticulture, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; School of Life Science, Zhejiang University, Hangzhou 310029, China |
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Abstract Fusarium head blight (FHB) caused by Fusarium graminearum is a devastating disease that results in extensive yield losses to wheat and barley. A green fluorescent protein (GFP) expressing plasmid pRP22-GFP was constructed for monitoring the colonization of two biocontrol agents, Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116, on the spikes of barley and their effect on suppression of FHB. Survival and colonization of the Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 strains on spikes of barley were observed by tracking the bacterial transformants with GFP expression. Our field study revealed that plasmid pRP22-GFP was stably maintained in the bacterial strains without selective pressure. The retrieved GFP-tagged strains showed that the bacterial population fluctuation accorded with that of the rain events. Furthermore, both biocontrol strains gave significant protection against FHB on spikes of barley in fields. The greater suppression of barley FHB disease was resulted from the treatment of barley spikes with biocontrol agents before inoculation with F. graminearum.
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Received: 10 March 2005
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