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Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology)  2015, Vol. 16 Issue (4): 275-285    DOI: 10.1631/jzus.B1400162
Articles     
Evaluation of yeasts from Tibetan fermented products as agents for biocontrol of blue mold of Nashi pear fruits
Hao Hu, Yang Xu, Huang-ping Lu, Rui Xiao, Xiao-dong Zheng, Ting Yu
Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute for Food Science, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
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Abstract  A total of 20 strains of yeast isolated from Tibetan fermented products were screened for antagonism against blue mold of pear caused by Penicillium expansum. Six isolates that inhibited incidence of postharvest decay by 35% or more were selected for further screening. Among them, the most effective was Rhodotorula mucilaginosa. The results showed that washed cell suspensions of R. mucilaginosa yielded better antagonistic efficacy than unwashed cell-culture mixtures, cell-free culture filtrates, and autoclaved cell cultures. Biocontrol activity improved with increasing concentrations of incubated cells. The best concentration was 1×108 cells/ml, at which the incidence of decay was only 16.7% after 6 d of incubation. The germination of conidia of P. expansum in vitro was significantly inhibited by both washed cell-suspensions and unwashed cell-culture mixtures. Rapid colonization by yeast at different concentrations showed a relationship between yeast-cell concentration and biocontrol activity. Although the titratable acidity of pear fruits increased after treatment, R. mucilaginosa did not affect the total soluble solids or ascorbic acid content. This is the first study to report that the yeast R. mucilaginosa from Tibet Autonomous Region of China may have potential as an antagonist to control the postharvest decay of pear fruits.

Key wordsBiocontrol      Postharvest      Rhodotorula mucilaginosa      Tibetan yeast isolates      Pear     
Received: 10 June 2014      Published: 03 April 2015
CLC:  S476+.9  
Cite this article:

Hao Hu, Yang Xu, Huang-ping Lu, Rui Xiao, Xiao-dong Zheng, Ting Yu. Evaluation of yeasts from Tibetan fermented products as agents for biocontrol of blue mold of Nashi pear fruits. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2015, 16(4): 275-285.

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http://www.zjujournals.com/xueshu/zjus-b/10.1631/jzus.B1400162     OR     http://www.zjujournals.com/xueshu/zjus-b/Y2015/V16/I4/275

[1] Dong-feng Liu, Dong Zhang, Guo-qin Liu, Sayed Hussain, Yuan-wen Teng. Influence of heat stress on leaf ultrastructure, photosynthetic performance, and ascorbate peroxidase gene expression of two pear cultivars (Pyrus pyrifolia)[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2013, 14(12): 1070-1083.
[2] Yan-ni YIN, Lei-yan YAN, Jin-hua JIANG, Zhong-hua MA. Biological control of aflatoxin contamination of crops[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2008, 9(10): 787-792.
[3] SONG Hui-zhi, WANG Jun, LI Yong-hui. Studies on vibration characteristics of a pear using finite element method[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2006, 7(6 ): 11-.
[4] ZHANG Xin, ZHANG Bing-xin, ZHANG Zhen, SHEN Wei-feng, YANG Ching-hong, YU Jing-quan, ZHAO Yu-hua. Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6(8): 770-777.
[5] LI Bin, XIE Guan-lin, SOAD A., COOSEMANS J.. Suppression of Meloidogyne javanica by antagonistic and plant growth-promoting rhizobacteria*[J]. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology), 2005, 6( 6): 9-.