Please wait a minute...
浙江大学学报(工学版)
浙江大学学报(工学版)
优秀论文推荐     
低场强脉冲电场杀灭水中大肠杆菌的实验研究
寇艳芹1, 郑超1,2, 徐羽贞1, 黄逸凡1, 刘振1, 闫克平1
1. 浙江大学 生物质化工教育部重点实验室,浙江 杭州 310027；2. 浙江省医疗器械研究所,浙江 杭州 310009
Water disinfection of E. coli by pulsed low electric fields
KOU Yan-qin1, ZHENG Chao1, 2, XU Yu-zhen1, HUANG Yi-fan1, LIU Zhen1, YAN Ke-ping1
1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, China; 2. Zhejiang Institute of Medical Device, Hangzhou 310009, China
 全文: PDF(1342 KB)  
摘要：

采用高频低场强脉冲电场系统对水中的大肠杆菌进行灭菌研究,探索反应器结构、电气参数及水质参数等对脉冲电场灭菌效率和能量效率的影响.圆筒式反应器体积为500 mL,平均脉冲场强为3～12 kV/cm,脉冲频率可以高达20 kHz.最佳高压电极结构为6层均匀分布的直径为40 mm的网电极.脉冲电场的杀菌效率和能量效率随着脉宽和场强的增大而提高,水的电导率影响波形和单脉冲能量的变化,从而影响杀菌效果,当初始细菌密度降低时有利于彻底杀光.脉冲频率对杀菌无明显影响,但高频率能够大幅缩短处理时间.当脉冲电压为6 kV,脉宽为30 μs,水的电导率为2.5 μS/cm,细胞初始密度为103～106 cfu/mL时,在消耗能量<200 J/mL,水温<40 ℃的前提下,脉冲电场处理后细菌密度下降了1、2个对数.当细菌初始密度低于1 250 cfu/mL时,细菌被全部杀光,实现了非热低温灭菌.
关键词： 消毒灭菌;  低场强;  高频;  水处理;  脉冲电场    
Abstract:

Water disinfection of E. coli by high frequency pulsed low electric fields was investigated. The effects of constructional, electrical and aqueous parameters on disinfection efficiency and energy efficiency were evaluated. Experiments were performed with a 500 mL cylindrical reactor. Average electric intensity of 3-12 kV/cm was applied and pulsed frequency was up to 20 000 Hz. Optimized structure of high voltage electrode were six equally distributed mesh electrodes with diameter of 40 mm. Both the disinfection efficiency and energy efficiency were enhanced by increasing the electric strength and pulse duration. Water conductivity could influence the pulsed waveforms and single pulse energy, which changed the disinfection results. Reducing the initial bacterial density made the process more effective and easily realized sterilization. Pulsed frequency did not show any effect on disinfection, but high frequency could greatly reduce treatment time. When pulsed voltage, pulsed width, water conductivity and initial bacteria density were 6 kV, 30 μs, 2.5 μS/cm and 103-106 cfu/mL, respectively, 1-2 logarithmic reduction were obtained with energy consumption less than 200 J/mL and water temperature lower than 40 ℃. If initial bacterial density was less than 1 250 cfu/mL, all the cells could be sterilized, which was a non-thermal processing.
Key words: pulsed electric field    disinfection    water treatment    low field intensity    high frequency
出版日期: 2014-12-08
:  TQ 9  
基金资助:

中央高校科研业务费资助项目(2012XZZX001).
通讯作者: 闫克平,男,教授,博导     E-mail: kyan@zju.edu.cn
作者简介: 寇艳芹（1987—）,女,硕士生,从事生物化工、低温等离子体及生物电工的研究.E-mail: kouyanqin8103@163.com
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　
寇艳芹
徐羽贞
刘振
郑超
黄逸凡
闫克平

引用本文:

寇艳芹, 郑超, 徐羽贞, 黄逸凡, 刘振, 闫克平. 低场强脉冲电场杀灭水中大肠杆菌的实验研究[J]. 浙江大学学报(工学版), 10.3785/j.issn.1008-973X.2014.10.000.

KOU Yan-qin, ZHENG Chao, XU Yu-zhen, HUANG Yi-fan, LIU Zhen, YAN Ke-ping. Water disinfection of E. coli by pulsed low electric fields. JOURNAL OF ZHEJIANG UNIVERSITY (ENGINEERING SCIENCE), 10.3785/j.issn.1008-973X.2014.10.000.

链接本文:

http://www.zjujournals.com/xueshu/eng/CN/10.3785/j.issn.1008-973X.2014.10.000    或    http://www.zjujournals.com/xueshu/eng/CN/Y2014/V48/I11/3

［1］ ZHANG Q, BARBOSA-CANOVAS G V, SWANSON B G. Engineering aspects of pulsed electric field pasteurization ［J］. Journal of Food Engineering, 1995, 25(2): 261-281.
［2］ MOSQUEDA-MELGAR J, RAYBAUDI-MASSILIA R M, MARTIN-BELLOSO O. Influence of treatment time and pulse frequency on Salmonella enteritidis, Escherichia coli and Listeria monocytogenes populations inoculated into melon and watermelon juices, treated by pulsed electric field ［J］. International Journal of Food Microbiology, 2007, 117(2): 192-200.
［3］ SAMPEDRO F, RODRIGO D, MARTINEZ A, et al. Review: application of pulsed electric fields in egg and egg derivatives ［J］. Food Science and Technology International, 2006, 12(5): 397-405.
［4］ JAEGER H, SCHULZ A, KARAPETKOV N, et al. Protective effect of milk constituents and sublethal injuries limiting process effectiveness during PEF inactivation of Lb. rhamnosus ［J］. International Journal of Food Microbiology, 2009, 134(1/2): 154-161.
［5］ AGUILO-AGUAYO I, OMS-OLIU G, SOLIVA-FORTUNY R, et al. Flavour retention and related enzyme activities during storage of strawberry juices processed by high-intensity pulsed electric fields or heat ［J］. Food Chemistry, 2009, 116(1): 59-65.
［6］ JIA M, ZHANG Q H, MIN D B. Pulsed electric field processing effects on flavor compounds and microorganisms of orange juice ［J］. Food Chemistry, 1999, 65(4): 445-451.
［7］ HEINZ V, TOEPFL S, KNORR D. Impact of temperature on lethality and energy efficiency of apple juice pasteurization by pulsed electric fields treatment ［J］. Innovative Food Science and Emerging Technologies, 2003, 4(2): 167-175.
［8］ HUANG K, TIAN H, GAI L, et al. A review of kinetic models for inactivating microorganisms and enzymes by pulsed electric field processing ［J］. Journal of Food Engineering, 2012, 111(2): 191-207.
［9］ WOUTERS P C, ALVAREZ I, RASO J. Critical factors determining inactivation kinetic by pulsed electric eld food processing ［J］. Trends in Food Science and Technology, 2001, 12(3/4): 112-121.
［10］ WAN J, COVENTRY J, SWIERGON P, et al. Advances in innovative processing technologies for microbial inactivation and enhancement of food safety-pulsed electric field and low-temperature plasma ［J］. Trands in Food Science and Technology, 2009, 20(9): 414-424.
［11］ SANCHEZ-MORENO C, ANCOS B, PLAZA L, et al. Nutritional approaches and health-related properties of plant foods processed by high pressure and pulsed electric fields ［J］. Critical Reviews in Food Science and Nutrition, 2009, 49(6): 552-576.
［12］ KEMPKES M A, LIANG R, PETRY J, et al. PEF systems for food and waste streams ［C］∥IEEE International Power Modulators and High Voltage Conference. Kas Vegas: IEEE, 2008: 73-76.
［13］ PURTOLAS, LPEZ N, CONDN S, et al. Pulsed electric fields inactivation of wine spoilage yeast and bacteria \[J\]. International Journal of Food Microbiology, 2009, 130(1): 49-55.
［14］ QIN B L, BARBOSA-CANOVAS G V, SWANSON B G, et al. Inactivating microorganism using a pulsed electric field continuous treatment system ［J］. IEEE Transactions on Industry Applications, 1998, 34(1): 43-49.
［15］ LI Y Q, CHEN Q, LIU X H, et al. Inactivation of soybean lipoxygenase in soymilk by pulsed electric fields ［J］. Food Chemistry, 2008, 109(2): 408-414.
［16］ DAMAR S, BOZOGLU F, HIZAL M, et al. Inactivation and injury of Escherichia coli O157: H7 and Staphylococcus aureus by pulsed electric fields ［J］. World Journal of Microbiology, 2002, 18(1): 16.
[1] 胡小东, 顾临怡, 张范蒙. 应用于数字变量马达的高速开关阀[J]. 浙江大学学报(工学版), 2016, 50(8): 1551-1560.
[2] 王冕, 曲东昌, 陈国柱. 三相脉冲调制变流器驱动电源电磁兼容性能提升[J]. 浙江大学学报(工学版), 2016, 50(4): 657-662.
[3] 杨广伟, 姜珺秋, 王琨, 赵庆良, 李伟. 生物产电人工湿地系统对处理生活污水的效能[J]. 浙江大学学报(工学版), 2015, 49(6): 1186-1193.
[4] 崔雨辰,段浩,张聪,吴筱敏. 高频交流电场的频率对预混稀燃火焰的影响[J]. 浙江大学学报(工学版), 2015, 49(5): 944-949.
[5] 寇艳芹, 郑超, 徐羽贞, 黄逸凡, 刘振, 闫克平. 低场强脉冲电场杀灭水中大肠杆菌的实验研究[J]. 浙江大学学报(工学版), 2015, 49(4): 806-812.
[6] 周慧慧, 叶苗苗, 张土乔, 邵煜. 紫外光降解水中氟比洛芬的动力学与机理[J]. 浙江大学学报(工学版), 2014, 48(8): 1376-1383.
[7] 郑超,徐羽贞,黄逸凡,刘振,闫克平. 脉冲等离子体射流杀灭表面和水中的细菌[J]. 浙江大学学报(工学版), 2014, 48(7): 1329-1335.
[8] 唐凯, 贾佳, 邓靖, 宋亚丽, 李青松. 超声及高锰酸钾-超声协同降解水中17β-雌二醇的影响因素分析[J]. 浙江大学学报(工学版), 2014, 48(6): 1132-1140.
[9] 丁晶,舒欣,赵庆良. 电化学氧化法处理氨氮废水的影响因素[J]. J4, 2013, 47(5): 889-894.
[10] 孟培培,张军明,陈恒林,钱照明. 反激变流器共模干扰的建模与预测[J]. J4, 2012, 46(9): 1702-1707.
[11] 甘智华,吴英哲,袁园,邱利民,张学军,张小斌,徐旭. 120 Hz单级脉管制冷机理论与实验[J]. J4, 2011, 45(11): 2014-2019.
[12] 甘智华,范炳燕,陈杰,等. 35K两级高频脉管制冷研究：Ⅱ.实验验证[J]. J4, 2009, 43(8): 1454-1457.
[13] 甘智华,范炳燕,徐娜娜,等. 35K两级高频脉管制冷研究:Ⅰ.理论分析[J]. J4, 2009, 43(8): 1448-1453.
[14] 蒋刚, 何闻, 郑建毅. 高频振动时效的机理与实验研究[J]. J4, 2009, 43(7): 1269-1272.
[15] 周俊虎 张国平 王智化 魏林生 游卓 岑可法. 高频介质阻挡放电产生臭氧的实验研究[J]. J4, 2008, 42(3): 502-505.