微光波对水葫芦水解糖化的促进机理研究

doi:10.3785/j.issn.1008-973X.2009.

2009, Vol. 43

Issue (7): 1337-1343 DOI: 10.3785/j.issn.1008-973X.2009.

能源与地质工程

微光波对水葫芦水解糖化的促进机理研究

程军,俞聪,宋文路,周俊虎,岑可法

（浙江大学能源清洁利用国家重点实验室,浙江杭州 310027）

Promotion mechanisms of microwave and lightwave pretreatments on enzymatic saccharification of water hyacinth

CHENG Jun, YU Cong, SONG Wen-lu, ZHOU Jun-hu, CEN Ke-fa

(State Key Laboratory of Clean Energy Utilization,Zhejiang University, Hangzhou 310027, China)

全文: PDF(1460 KB)

摘要：

利用色质联机和扫描电镜等微观分析手段,研究了微波时间、功率和光波组合等对水葫芦理化性质、还原糖产量和水解副产物的影响规律.水葫芦叶子经过蒸汽加热和NaOH预处理后,酶水解的还原糖产量仅为239 mg（每100 mg底物）；而联合了微光波预处理后,可提高到311 mg（每100 mg底物）,使还原糖产量提高了301%.在微波和光波组合比例为30∶70（总功率700 W）、加热时间1 min时,得到了554%的理论最大还原糖产量.当微波功率过高或时间过长时,在预处理阶段由半纤维素水解生成的大量木糖进一步分解,导致乙酸、丁酮和糠醛等有害小分子副产物增加,对后期酶水解糖化和发酵产酒精造成不利影响.

关键词： 水葫芦; 微光波; 酶水解; 还原糖

Abstract:

The gas chromatography-mass spectrometry (GC-MS) and scanning electron microscope (SEM) were used to study the effects of microwave time, power and combination with lightwave on the hyacinth (eichhornia crassipes) physicochemical properties, reducing sugar yields and hydrolysis byproducts. The reducing sugar yield of only 239 mg/100 mg hyacinth leave in the enzymatic hydrolysis was obtained when it was pretreated with the steam explosion and NaOH solution. The reducing sugar yield was increased by 301% to 311 mg/100 mg hyacinth leave when the microwave and lightwave pretreatments were additionally employed. The 554% of potential maximum sugars was released when the hyacinth leave was pretreated with the power ratio of microwave to lingtwave at 30∶70 (total power is 700 W) for 1 min. When the microwave power was higher and time was longer, more hydrolysis byproducts such as acetic acid, butanone and furfural were produced due to the further decomposition of the xylose derived from the hemicellulose hydrolysis, which was harmful to the following enzymatic saccharification and fermentative ethanol fuel．

Key words: water hyacinth microwave and lightwave enzymatic hydrolysis reducing sugars

出版日期: 2009-08-01

TQ352

基金资助:

浙江省重大科技专项资助项目(2008C13023-3)；浙江省科技计划资助项目(2006C33049)；国家“863”高技术研究发展计划资助项目(2006AA05Z122)；国家自然科学基金资助项目(50406022)；全国优秀博士学位论文作者专项基金资助项目(200437)；霍英东高校青年教师基金资助项目(104021).

作者简介: 程军(1974-),男,山东济宁人,副教授,博士,从事生物质能等可再生能源研究.

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	程军
	俞聪
	宋文路
	等

引用本文:

程军, 俞聪, 宋文路, 等. 微光波对水葫芦水解糖化的促进机理研究[J]. J4, 2009, 43(7): 1337-1343.

CHENG Jun, SHU Cong, SONG Wen-Lu, et al. Promotion mechanisms of microwave and lightwave pretreatments on enzymatic saccharification of water hyacinth. J4, 2009, 43(7): 1337-1343.

链接本文:

http://www.zjujournals.com/xueshu/eng/CN/10.3785/j.issn.1008-973X.2009. 或 http://www.zjujournals.com/xueshu/eng/CN/Y2009/V43/I7/1337

［1］王树荣,郑赟,骆仲泱,等. 生物质组分热裂解动力学研究［J］. 浙江大学学报:工学版,2007, 41(4): 585588．
WANG Shu-rong, ZHENG Yun, LUO Zhong-yang, et al. Kinetic study on pyrolysis of biomass components［J］. Journal of Zhejiang University: Engineering Science, 2007, 41(4): 585588．
［2］ MISHIMA D, TATEDA M, IKE M, et al. Comparative study on chemical pretreatments to accelerate enzymatic hydrolysis of aquatic macrophyte biomass used in water purification processes ［J］. Bioresource Technology, 2006, 97(16): 21662172．
［3］ MISHIMA D, KUNIKI M, SEI K, et al. Ethanol production from candidate energy crops: water hyacinth (eichhornia crassipes) and water lettuce (pistia stratiotes L) ［J］. Bioresource Technology, 2008, 99(7): 24952500．
［4］ ABRAHAM M, KURUP G M. Pretreatment studies of cellulose wastes for optimization of cellulase enzyme activity ［J］. Applied Biochemistry and Biotechnobgy, 1997, 62(2-3): 201211．
［5］ NIGAM J N. Bioconversion of water-hyacinth (eichhornia crassipes) hemicellulose acid hydrolysate to motor fuel ethanol by xylose–fermenting yeast ［J］. Journal of Biotechnology, 2002, 97(2): 107116．
［6］ BALAT M, BALAT H, OZ C. Progress in bioethanol processing ［J］. Progress in Energy and Combustion Science, 2008, 34(5): 551573．
［7］ HU Z H, WEN Z Y. Enhancing enzymatic digestibility of switchgrass by microwave-assisted alkali pretreatment ［J］. Biochemical Engineering Journal, 2008, 38(3): 369378．
［8］ SAHA B C, BISWAS A, COTTA M A. Microwave pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol ［J］. Journal of Biobased Materials and Bioenergy, 2008, 2(3): 210217．
［9］ ZHU S D, WU Y X, YU Z N, et al. Comparison of three microwave/chemical pretreatment processes for enzymatic hydrolysis of rice straw ［J］. Biosystems Engineering, 2006, 93(3): 279283．
［10］ SCHELL D, NGUYEN Q, TUCKER M, et al. Pretreatment of softwood by acid-catalyzed steam explosion followed by alkali extraction ［J］. Applied Biochemistry and Biotechnology, 1998, 70-72(1): 1724．
［11］ YAN L F, LI W, YANG J L, et al. Direct visualization of straw cell walls by AFM ［J］. Macromolecular Bioscience, 2004, 4(2): 112118．

[1]	沈雪亮夏黎明. 共固定化发酵纤维素水解液产乳酸的研究[J]. J4, 2007, 41(5): 823-826+863.
[2]	庄新姝王树荣安宏骆仲泱岑可法. 纤维素低浓度酸水解制取液体燃料的试验研究[J]. J4, 2006, 40(6): 997-1001.

Viewed

Full text

Abstract

Cited

Shared

Discussed