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浙江大学学报(工学版)
JOURNAL OF ZHEJIANG UNIVERSITY (ENGINEERING SCIENCE)  2017, Vol. 51 Issue (7): 1317-1323    DOI: 10.3785/j.issn.1008-973X.2017.07.007
Civil Engineering     
Radial distribution shifts of microbial communities in biofilms developed under stagnation condition in water supply pipes
SHENTU Hua-bin1, LIU Jing-qing1, YE-Ping2, LI Hang-jia2, XU Bing2, PENG Hong-xi1, WANG Lei3
1. College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, China;
2. JiaYuan Water Supply and Sewerage Limited Company, Jiaxing 314000, China;
3. Architectural Engineering Institute, Zhejiang University of Technology, Hangzhou 310014, China
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Abstract  

Subject depends on the pilot platform in actual water supply system to conduct research in order to analyze radial distribution shifts of microbial communities and numbers of total bacteria in biofilms developed under stagnation condition in water supply pipes. Scanning electron microscope (SEM) was used to observe biofilms morphology. Biofilm community in different spatial pipe locations was collected and systematically investigated using the high-throughput sequencing of 16S rRNA gene by real-time fluorescence quantitative (RT-PCR) to count total number of bacteria in the biofilm. Results showed that the increase of total bacteria in the upper of pipe was little, but in the middle and bottom was significant. Radial distribution of microbial communities in the high density polyethylene pipe (HDPE) inner wall was uniform and the content changed little under stagnation condition, but as for ductile iron pipe(DCIP), the microbial communities increased sequentially from top to bottom and the change was distinct. The content of microbial community in the middle of pipe was dramatically changing, and the effect of microbial community in the region on pipe corrosion should be concerned.

Received: 09 June 2016      Published: 08 July 2017
CLC:  TU991  
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Cite this article:

SHENTU Hua-bin, LIU Jing-qing, YE-Ping, LI Hang-jia, XU Bing, PENG Hong-xi, WANG Lei. Radial distribution shifts of microbial communities in biofilms developed under stagnation condition in water supply pipes. JOURNAL OF ZHEJIANG UNIVERSITY (ENGINEERING SCIENCE), 2017, 51(7): 1317-1323.

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http://www.zjujournals.com/eng/10.3785/j.issn.1008-973X.2017.07.007     OR     http://www.zjujournals.com/eng/Y2017/V51/I7/1317


滞流前后管道生物膜微生物群落径向分布差异

为了探究供水管道管壁生物膜在滞流前、后的细菌数量和微生物群落结构在空间分布上变化的差异性，依托建设于实际供水管网系统中的管网中试平台开展研究.采用电子扫描电镜（SEM）观察管道生物膜的形态差异，采用实时荧光定量PCR计数生物膜中细菌总数，采用高通量测序分析管道不同空间位置上微生物群落种类和含量.研究结果表明，滞流后细菌总数在两种管道上部增加较少，中部和下部增加较大.微生物群落在高密度聚乙烯管道内壁空间位置上分布较均匀，种群结构变化不大，球墨铸铁管中的种群结构分布变化较大.其中腐蚀菌属从空间位置的上部至下部含量依次增加，滞流后管道中部腐蚀菌落含量变化较大，应重点关注管道中部腐蚀菌落对管道腐蚀的影响.

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