Design of electrochemical biosensors based on diffusion kinetics
YANG Hao, YANG Xiao-he, CHEN Yu-quan, PAN Min
1. State Specialized Laboratory of Biomedical Sensors, Department of Biomedical Engineering,
Zhejiang University, Hangzhou, 310027, China; 2. Zhejiang Medical Device Institute, Hangzhou, 310027, China
When electrochemical biosensors are applied in fluidflowing small sample testing, the testing result is not stable. It is because of the influence of micro-reactors‘ channel height on electrode doublelayer and diffusion kinetics. So a new design conception is proposed. Through the analysis of hydrokinetics and diffusion kinetics in microchannel, the minimum diffuse layer’s thickness of coplanar electrodes in unstable state diffusion process of immobile liquid was deduced. Based on it, two kinds of glucose oxidase electrodes with different channel height were manufactured and contrasted test was done with glucose oxidase electrodes with no channel. The result indicates that only when microreactors channel height is higher than the minimum diffuse layers thickness of coplanar electrodes in unstable state diffusion process of immobile liquid, unstable state diffusion process is not disturbed by external factors and enzymatic reaction goes normally.
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