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JOURNAL OF ZHEJIANG UNIVERSITY (ENGINEERING SCIENCE)
    
Influence of doping and molecular modification on cell compatibility of diamond like carbon film
LIU Min1, LIU Ai ping1,2, YU Jian can1, QIAN Guo dong1
1. State Key Laboratory of Silicon Materials, School of Material Science and Engineering, Zhejiang University, Hangzhou 310027, China; 2. Department of Physics, Zhejiang Sci Tech University, Hangzhou 310018, China
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Abstract  

DLC film was functionalized by element doping and molecular modification aiming at the biocompatibility regulation of diamond like carbon (DLC) film for better use in different environment as the substrate material of cell sensor. X ray photoelectron spectroscopy, Raman spectroscopy and static state contact angle measurement were applied to characterize the surface morphology and microstructure of DLC film. The effects of element doping and molecular modification on the cell compatibility of the film were explored. Results show that the doping of B, N, P and Pt elements and the modification of 3 aminobenzeneboronic acid (APBA) do not change the amorphous structure of DLC film, but enhance the ordered structure. The doping of B, N and P elements decreases the contact angle of DLC film, while the codoping of N and Pt elements does not lead to obvious change of  contact angle. The APBA modification makes the contact angle decrease for different DLC films. Moreover, the doping of B, N and P elements promotes the adhesion and proliferation of PC12 cells and inhibits cell apoptosis. However, the incorporation of Pt element presents the opposite effect. The modification of 3 aminophenylboronic acid can obviously promote the cell growth on the doped DLC surface. Therefore, element doping and molecular modification are effective methods for biocompatibility modulation of DLC film.



Published: 15 October 2015
CLC:  TB 321  
  Q 24  
Cite this article:

LIU Min, LIU Ai ping, YU Jian can, QIAN Guo dong. Influence of doping and molecular modification on cell compatibility of diamond like carbon film. JOURNAL OF ZHEJIANG UNIVERSITY (ENGINEERING SCIENCE), 2015, 49(9): 1790-1795.

URL:

http://www.zjujournals.com/eng/10.3785/j.issn.1008 973X.2015.09.024     OR     http://www.zjujournals.com/eng/Y2015/V49/I9/1790


掺杂及分子修饰对类金刚石细胞相容性的影响

为实现类金刚石(DLC)薄膜在不同使用环境下生物相容性的可调性,采用元素掺杂及化学分子修饰对DLC薄膜表面进行改性,借助X射线光电子能谱、拉曼光谱及静态接触角测试表征薄膜的表面形貌和微观结构,探究掺杂及分子修饰对薄膜细胞相容性的影响.结果显示:B、N、P、Pt元素掺杂以及3 氨基苯硼酸(APBA)修饰均没有改变薄膜非晶结构,只是提高薄膜有序程度.B、N、P元素的掺入减小了DLC薄膜的接触角,而N、Pt元素共掺使接触角变化不大.APBA修饰使各种DLC薄膜的接触角均减小.B、N、P元素的掺入促进PC12细胞的黏附与增殖,抑制细胞凋亡,而Pt元素的掺入起相反的作用,APBA修饰对细胞生长有明显促进作用.这说明元素掺杂及分子修饰是调节DLC薄膜细胞相容性的有效方法.

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