| Biological sciences & biotechnology |
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| Comparison of esterase isozymes in two color varieties of Tenebrio molitor L . |
| HUANG Qiong１ ,２ , HU Jie３ , GOU Lin４ |
| 1 .The Provincial Key Laboratory of Forest Protection , Forestry College , Sichuan A gricultural University , Y a′ an , Sichuan 625014 , China ; 2 . Forestry Ecological Engineering in the Upper Reaches of Y angtze River Key Laboratory of Sichuan Province , Forestry College ,Sichuan A gricultural University , Y a′ an , Sichuan 625014 , China ; 3 . College of Economics and Management , Sichuan A gricultural University , Chengdu 611130 , China ; 4 . Department of Biology ,College of Li fe Science , Sichuan A gricultural University , Y a′an , Sichuan 625014 , China |
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Abstract In order to provide some references for studying the genetic variation and selecting varieties of Tenebrio molitor , the esterase isozyme zymograms and activity at different developmental stages of the yellow‐ and black‐color varieties of T . molitor were compared using vertical polyacrylamide gel electrophoresis and spectrophotometry . The results showed that １５ enzyme bands with different mobility were found at all , which were distributed in five zones . The larvae and adults of the black‐color variety
showed one more distinctive band compared with those of the yellow larvae and adults , respectively . Moreover , band numbers , migration rate of enzyme bands and strength of enzymatic activity at differentdevelopmental stages of both color varieties varied greatly . Compared with other developmental stage ,band numbers of esterase isozyme were the least ( only four bands) and enzymatic activity was the weakest at egg stage , and two of four bands were found only at this stage . The enzymatic activities of carboxylesterase ( CarE ) , acetylcholine esterase ( AchE ) , acid phosphatase ( ACP ) and alkaline phosphatase (AKP) in black‐color variety were always higher than those in yellow‐color variety at all stages from egg to adult . While in the same variety , The enzymatic activity of CarE , AchE , ACP and AKP varied in the order of egg stage ＜ adult stage ＜ pupal stage ＜ larval stage . The results above build up some underlying data for further studying the insecticide resistance and variety selection of the
mealworm beetles .
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Published: 25 November 2011
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2 种色型黄粉虫酯酶同工酶的比较
采用不连续聚丙烯酰胺凝胶垂直板电泳并结合分光光度法,对黄、黑２ 种色型黄粉虫( Tenebrio molitor L .)各发育期的酯酶同工酶酶谱及酶活性进行比较研究.结果表明:在２ 种色型黄粉虫的各发育期,共发现迁移率不同的酯酶同工酶酶带１５ 条,这些酶带分布于５ 个不同的泳动区;黑色型的幼虫和成虫分别比黄色型的幼虫和成虫各多１ 条特征性酶带;同一色型黄粉虫各发育期酯酶同工酶的酶带数、酶活性强弱及迁移率也变化较大,卵期的酶带数最少(４ 条) 、最弱,其中２ 条为卵期的特有性酶带.酶活性检测进一步表明:从卵至成虫期,黑色型黄粉虫的羧酸酯酶(CarE) 、乙酰胆碱酯酶( AchE) 和磷酸酯酶(ACP 和AKP)活性均比黄色型黄粉虫高;同一色型黄粉虫各发育阶段的CarE 、AchE 及ACP 和AKP活性也呈现明显的规律性变化,即:卵期＜ 成虫期＜ 蛹期＜ 幼虫期.上述结果为黄粉虫的抗药性研究及品种选育积累了一定的基础数据.
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