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浙江大学学报（农业与生命科学版）
  2011, Vol. 37 Issue (6): 617-623    DOI: 10.3785/j.issn.1008-9209.2011.06.005
Biological sciences & biotechnology     
Screening of a novel bioflocculant‐producing strain of Bacillus sp . and research for its flocculation characteristics
CAO Fang , YU Xiu‐mei , GUO Juan‐li , HE Min , PAN Kang‐cheng
Key Laboratory of Animal Disease & Human Health of Sichuan Province , College of Veterinary Medicine , Sichuan Agricultural University , Y a′an , Sichuan 625014 , China
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Abstract  A novel bioflocculant‐producing Bacillus sp . was isolated from soil by streak plate method , and screened out by an index that the fermentation broth flocculated kaolin suspension . The strain was identified by physiological and biochemical characteristics and １６S rDNA sequence analysis . The effect of the factors such as fermentation broth dosage , Ca２ ＋ concentration and pH on the flocculation were investigated . The results revealed that a novel bioflocculant‐producing bacterium , named MBFF６ , was isolated from soil and identified as Bacillus megaterium ; its flocculating rate could achieve ９５.７％ ; the optimal condition for flocculation was as follows : ermentation broth dosage of ２％ , Ca２ ＋ concentration of ４.５ mmol.L － １ and pH ７ .０The bioflocculant was produced during the bacterial growth and was an extracellular product which was distributed in the culture broth.

Published: 25 November 2011
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YU Xiu‐mei
GUO Juan‐li
HE Min
PAN Kang‐cheng
CAO Fang
Cite this article:

CAO Fang,YU Xiu‐mei,GUO Juan‐li,HE Min,PAN Kang‐cheng. Screening of a novel bioflocculant‐producing strain of Bacillus sp . and research for its flocculation characteristics. , 2011, 37(6): 617-623.

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http://www.zjujournals.com/agr/10.3785/j.issn.1008-9209.2011.06.005     OR     http://www.zjujournals.com/agr/Y2011/V37/I6/617


高絮凝活性芽孢杆菌的筛选及其絮凝特性研究

采用平板分离法从土壤中分离芽孢杆菌,以发酵液对高岭土悬液絮凝效果为指标筛选高絮凝 活性菌株,并通过细菌形态、生理生化特性和１６S rDNA 序列分析进行鉴定;同时考察发酵液加入量、Ca２ ＋ 浓度和pH 对絮凝效果的影响以及微生物絮凝剂在发酵液中的分布.结果表明:从土壤中分离筛选 到１ 株具有高效絮凝活性的芽孢杆菌MBFF６ ,其絮凝率可达９５ .７％ ;根据细菌菌落形态、生理生化特性和１６S rDNA 序列分析,确定该菌株为巨大芽孢杆菌;其最佳絮凝条件为加入的菌液体积分数２％ ,Ca２ ＋ 浓度４.５ mmol.L － １ ,pH ７.０ ;该微生物絮凝剂是细菌在生长过程中产生的胞外代谢产物,存在于发酵液中.
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